Functional state of cardiomyocyte mitochondria in malignant process in presence of comorbid pathology in experiment

Frantsiyants, Elena Mikhaylovna; Cheryarina, Natalia D.; Surikova, Ekaterina I.; Шихлярова, А. И.; Бандовкина, В. А.; Nemashkalova, Ludmila A.; Kaplieva, Irina V.; Trepitaki, Lidia K.; Качесова, П. С.; Котиева, И. М.; Morozova, M.I.; Pogorelova, Yulia A.

doi:10.37748/2686-9039-2021-2-3-2

Cited by 19 publications

(17 citation statements)

References 24 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase in the content of MDA was also demonstrated in the mitochondrial fraction of the heart in the female rats with DM by a factor of 1.9 and in those with the growth of Guerin's carcinoma against the background of DM by a factor of 1.7 [19,20].…”

Section: Resultsmentioning

confidence: 99%

Influence of diabetes mellitus on free radical processes in the heart in rats with Guerin’s carcinoma and characteristics of malignant lesions depending on the gender of animals

Frantsiyants¹,

Goroshinskaya²,

Kaplieva³

et al. 2022

View full text Add to dashboard Cite

To study the intensity of lipid peroxidation (LPO) and the activity of the main antioxidant protection enzyme: superoxide dismutase (SOD) in heart tissues and tumors in rats of both genders with Guerin’s carcinoma (GC) and the tumor growth against the background of diabetes mellitus (DM). Materials and methods. Our research work was carried out in 80 outbread albino male and female rats, divided into 4 groups, with 10 animals of each gender in a group. The animals of two groups, namely, an intact animal group and a group of rats treated with alloxan DM (with a 5-fold increase in glucose levels) were subcutaneously transplanted with the Guerin’s carcinoma (GC) strain cells, and at the same time we used one group of the intact rats and another group of the animals with DM as the references. The content of malondialdehyde (MDA), diene conjugates (DC), and the SOD activity in the heart and the tumor tissues were determined by conventional spectrophotometric methods. Results. The most pronounced changes were found in the heart in the female rats with isolated GC and GC growing against the background of DM: a more than threefold increase in MDA, with a significant increase in DC and a multiple increase in the SOD activity as compared with the intact animals. In the GC tissue, the dependence of the severity of the increase in the MDA content on the size of the tumor was traced: the maximum increase in both parameters was observed in males with GC tumor growing against the background of DM. In the males, the volume of the subcutaneous tumor nodes was 1.8 times greater than that in the reference group and in the females with combined pathology, while in the females with combined pathology the volume of their tumors was 1.3 times less than that in the reference group, although the area of tumor lesions in them was maximum due to extensive metastasizing. Conclusion. DM has changed the specifics of oncogenesis depending on the gender of the animals. The identified gender differences in the redox status of the heart and the tumor in rats with combined pathology contribute to specifics of oncogenesis in males and females and determines their life expectancy.

show abstract

Section: Resultsmentioning

confidence: 99%

Influence of diabetes mellitus on free radical processes in the heart in rats with Guerin’s carcinoma and characteristics of malignant lesions depending on the gender of animals

Frantsiyants¹,

Goroshinskaya²,

Kaplieva³

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It becomes obvious that at the final stage of melanoma development, the genetically determined inhibition of the progression of the malignant tumor, which forms multiple metastases in the lungs, accompanied by hemorrhages, involution of organs, is also canceled, resulting in accelerated death of the animals. It is accompanied by changes in different indicators and parameters at different hierarchical levels in an organism [19].…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of cancellation by pain of genetically determined inhibition of a malignant tumor growth in experiment

Frantsiyants¹,

Shikhlyarova²,

Котиева³

et al. 2022

View full text Add to dashboard Cite

Aims are to study the nature of the processes of carcinogenesis of experimental B16/F10 melanoma in uPA gene knockout mice modified by chronic neurogenic pain and investigate some electrophysiological mechanisms of melanoma development. Materials and methods. We used 48 C57BL/6-PlautmI. IBug-ThisPlau6FDhu/GFDhu mice of both genders with urokinase gene knockout and 102 C57BL/6 mice of both genders with the normal genotype. Chronic neurogenic pain (CNP) was produced due to bilateral ligation of the sciatic nerve. Against the above background, all animals were transplanted with B16/F10 melanoma. To study the mechanism of CNP, studies of the intracellular electrophysiological activity of neurons of the central nervous system of the snail Helix pomatia in the body in vivo were carried out. CNP was reproduced by dosed pressing of four main nerves with Fresnel hairs that with time turned into increasing pain. Membrane potential (MP), action potential (AP) and firing rate (FR) parameters of intracellular bio-potentials of the command neuron RPaG3, continuously recorded using an ultrathin glass microelectrode for 4-5 days, were analyzed. Results. It was detected that an activation of cancerogenesis during the modification of the progression of experimental B16/ F10 melanoma in C57BL/6-PlautmI.IBug-ThisPlau6FDhu/GFDhu mice with uPA gene knockout using CNP is accompanied by a 2-fold acceleration in the time of tumor production, stimulation of the growth of the primary tumor nodes from 1.05±0.08 cm3 to 9.50±0.98 cm3 (p<0.001) and multiple metastasizing to the lungs, a reduction in the life span from 34.67±0.67 to 21.33±2.19 days (p<0.001) <0.05) in the genetically modified mice, by changing some gender-specific characteristics of the progression of the malignant process. The neuropathic nature of pain resulting from command neuron compression or ligation of the sciatic nerves is essentially identical to the implementation of genetic programs responsible for the control of life and death, reproducing events in carcinogenesis with the progression of a malignant tumor. Conclusion. The initiation and chronization of pain at the local level of the nervous system can lead to generalization of the pain syndrome and contribute to the cancellation of genetically predetermined programs of carcinogenesis.

show abstract

“…As to the scale of modern medical problems, such as the growth of oncological, cardiovascular diseases, the pandemic of coronovirus infection, there is a significant increase in the incidence of diabetes mellitus (DM), which is one of the most topical pathologies that entails profound metabolic shifts at different levels of a self-organization organism: from the molecular to the systemic one. The clinical course of DM is accompanied by the emergence of many risk factors: the development of nephropathy and retinopathy, the determination of multiple vascular complications, up to the coronary, carotid, and peripheral vessels [8,9]. The main pathological changes in DM affect the biochemical environment of the body and are characterized by dysregulation of energy metabolism (glucose, amino acids and fatty acids), insulin resistance, rapid development of oxidative stress, and inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…The main pathological changes in DM affect the biochemical environment of the body and are characterized by dysregulation of energy metabolism (glucose, amino acids and fatty acids), insulin resistance, rapid development of oxidative stress, and inflammation. This leads to various complications and entails functional and morphological damage to target organs, such as the heart, the kidneys, the liver, the eyes, the reproductive and other organs [8,9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between comorbid pathology and tumor progression. Morphological portrayal of internal organs in modeling the growth of Guerin’s carcinoma under diabetic conditions

Shikhlyarova¹,

Frantsiyants²,

Kaplieva³

et al. 2022

View full text Add to dashboard Cite

Topicality. An increase in the incidence of malignant tumors progressing against the background of various comorbid pathologies determines the need to study the mutual influence of pathological processes using experimental modeling. Such models, for example, can reproduce the tumor growth modified by the comorbid condition of diabetes mellitus, the incidence rate of which is now increasing exponentially. In this case, a clear demonstration of changes in the structure of organs outside the zone of the primary tumor node, using data on the direct growth of the tumor and the content of diabetes markers therein and in the perifocal zone, can serve as an evidence-based argument for the implementation of the mechanism of modified tumor progression. The aim of our research work is to assess the state of the histological structure of some internal organs (the kidneys, the ovaries, the peritoneum) when modeling the main pathological process in animals, the growth of Guerin’s carcinoma, against the background of a comorbid state of experimental diabetes. Materials and methods. We used 32 outbred male and female rats weighing 180-220 g to reproduce the model of experimental diabetes by a single intraperitoneal injection of alloxan at a dosage of 150 mg/kg of body weight. One week after the production of persistent hyperglycemia in the range of 25.4±1.2 mmol/l, the animals were transplanted with Guerin’s carcinoma subcutaneously in the region of the right shoulder blade. Upon expiration of 2 weeks, the animals were decapitated, and the harvested organs were prepared according to the practice stages of morphological preparation for staining sections with hematoxylin-eosin, followed by morphological examination of the structure with the use of the Leica DM LS2 microscope with the Olympus optical. C-5050 Zoom video camera and the Morfotest software. Photographing was carried out with magnification x10, x40, x100. Results. Our study of the morphological portrayal of the ovary, the kidney, the visceral and parietal peritoneum bears witness to the identity of the changes, consisting in a total metastatic lesion and abnormal transformation of the normal structure of all the studied organs only in the female rats, when modeling the comorbid state of diabetes mellitus. At the same time, the aggressive nature of the tumor progression was manifested in the blood filling of the vessels and hemorrhage, followed by the release of tumor cells, their settlement, the enhanced proliferation, the formation of strands and compaction of cell aggregations throughout the volume of the organ. Some gender specific features of the tumor progression were noted, which were found in the female rats along the path of active metastasizing in case of small primary tumors, and in the male rats along the path of stimulating the growth of the primary focus without metastasizing. It was revealed that these differences are associated with different degrees of saturation of the tumor and perifocal zone with glucose, and they are determined by the state of the insulin/insulin-like growth factor (IGF) axis. Conclusion. Morphological examination of the organs affected by metastatic Guerin’s carcinoma in the female rats with diabetes mellitus makes it possible to detect not only the synergy of both pathological processes, but also a powerful pro-oncogenic effect of the comorbid state of diabetes in the implementation of the tumor growth program.

show abstract

Functional state of cardiomyocyte mitochondria in malignant process in presence of comorbid pathology in experiment

Cited by 19 publications

References 24 publications

Influence of diabetes mellitus on free radical processes in the heart in rats with Guerin’s carcinoma and characteristics of malignant lesions depending on the gender of animals

Influence of diabetes mellitus on free radical processes in the heart in rats with Guerin’s carcinoma and characteristics of malignant lesions depending on the gender of animals

Mechanisms of cancellation by pain of genetically determined inhibition of a malignant tumor growth in experiment

Relationship between comorbid pathology and tumor progression. Morphological portrayal of internal organs in modeling the growth of Guerin’s carcinoma under diabetic conditions

Contact Info

Product

Resources

About