Functional star polymers as reagents for efficient nucleic acids delivery into HT-1080 cells

Fus-Kujawa, Agnieszka; Teper, Paulina; Botor, Malwina; Klarzyńska, Katarzyna; Sieroń, Łukasz; Verbelen, Bram; Smet, Mario; Sieroń, Aleksander; Mendrek, Barbara; Kowalczuk, Agnieszka

doi:10.1080/00914037.2020.1716227

Cited by 17 publications

(26 citation statements)

References 38 publications

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“…In turn, reporter genes are the perfect tool for transfection results analysis and their expression can be easily monitored. Usage of reporter genes such green fluorescent protein (GFP), luciferase and β-galactosidase has been demonstrated to be very useful (Mülhardt and Beese, 2007;Smale, 2008;Masser et al, 2016;Mendrek et al, 2018;Fus-Kujawa et al, 2021). Moreover, these reporter genes may be also used for standardization of transfection for its efficiency based on expression levels of their products.…”

Section: Transfection Analysesmentioning

confidence: 99%

“…Nucleic acid is taken by endocytosis and pinocytosis that allows to keep membrane architecture intact. Whole procedure may be performed in the presence of serum in cell medium that is an obstacle in different transfection methods ( Santori et al, 2006 ; Fus-Kujawa et al, 2021 ).…”

Section: Chemical Transfectionmentioning

confidence: 99%

“…The choice of the optimal transfection method depends on factors including the type and origin of cells and the form of introduced nucleic acids ( Mirska et al, 2005 ; Fus-Kujawa et al, 2021 ). There are various strategies for introducing nucleic acids into cells that utilize variety of biological, chemical, and physical methods.…”

Section: Introductionmentioning

confidence: 99%

“…The chemical transfection methods are techniques that catalyze DNA cross-membrane transport through the use of Ca 2+ phosphate, polycations or dendrimers. The physical transfection approaches include microinjection, optical transfection, biolistic transfection and electroporation ( O’Brien and Lummis, 2011 ; Elsner and Bohne, 2017 ; Fus-Kujawa et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

Fus-Kujawa

Prus

Bajdak-Rusinek

et al. 2021

Front. Bioeng. Biotechnol.

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Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to DNA, the origin, type and the passage of transfected cells, and the presence or absence of serum in the cell culture. So far a number of transfection methods that use viruses, non-viral particles or physical factors as the nucleic acids carriers have been developed. Among non-viral carriers, the cationic polymers are proposed as the most attractive ones due to the possibility of their chemical structure modification, low toxicity and immunogenicity. In this review the delivery systems as well as physical, biological and chemical methods used for eukaryotic cells transfection are described and discussed.

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Section: Transfection Analysesmentioning

confidence: 99%

Section: Chemical Transfectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

Fus-Kujawa

Prus

Bajdak-Rusinek

et al. 2021

Front. Bioeng. Biotechnol.

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“…HT-1080 cells originate from human fibrosarcoma, a tumor from fibrous connective tissue. They are often used as models in transfection studies performed with various gene delivery systems [42][43][44]. The viability of the HT-1080 cells was assessed by an Alamar Blue reduction test assay.…”

Section: Comparison Of Iprox/mox/ei Copolymers With Other Polymers Usmentioning

confidence: 99%

Selective Partial Hydrolysis of 2-isopropyl-2-oxazoline Copolymers towards Decreasing the Ability to Crystallize

et al. 2020

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Poly(2-isopropyl-2-oxazoline) (PiPrOx) is readily prone to crystallization both in solid and from solutions. This feature is detrimental for certain applications. Here, we examine whether the presence of unsubstituted ethyleneimine (EI) units, a gradient distributed within a polymer chain composed of 2-isopropyl-2-oxazoline (iPrOx) and 2-methyl-2-oxazoline (MOx) units, decreases the ability to crystallize the copolymer and affects thermal properties compared to the homopolymer of iPrOx. We assumed that the separation of stiff iPrOx units by the more flexible EI will affect the spatial arrangements of the ordered chains, slightly plasticize and, as a result, decrease their ability to crystallize. The selective hydrolysis of gradient iPrOx and 2-methyl-2-oxazoline (MOx) copolymers, carried out under mild conditions, led to iPrOx/MOx/EI copolymers. To the best of our knowledge, the selective hydrolysis of these copolymers has never been carried out before. Their thermal properties and crystallization abilities, both in a solid state and from an aqueous solution, were analyzed. Based on the analysis of polymer charge and cytotoxicity studies, the potential use of the copolymers obtained was indicated in some biological systems.

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Poly(oligoethylene glycol methacrylate) Star‐Shaped Copolymers with Hydroxypropyl Methacrylate Cores

Sentoukas

Foryś

Marcinkowski

et al. 2022

Macro Chemistry & Physics

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Star-shaped copolymers with crosslinked hyperbranched cores, varying in both number and length of the arms, are prepared by the reversible addition-fragmentation chain transfer (RAFT) polymerization technique. Star synthesis is conducted via a simple, "core-first" method. Hydroxypropyl methacrylate monomer is used for the formation of the hyperbranched/crosslinked core, with ethylene glycol dimethacrylate used as a bifunctional monomer in the role of the cross-linker. Two different cross-linker/chain transfer agent ratios are chosen to manipulate the number of the arms in the final star nanostructure. Oligoethylene glycol methacrylate (OEGMA 500 ) is used as a biocompatible and hydrophilic component for the creation and extension of the arms. Arm length is controlled by the amount of OEGMA monomer used in the second polymerization step. Molecular characterization of the four synthesized stars is achieved via size exclusion chromatography and nuclear magnetic resonance, which provides information regarding the molar mass, dispersity, and composition of each star-shaped copolymer. Dynamic light scattering (DLS) studies of copolymers in N,N-dimethyl formamide solutions provide sizes of individual copolymer molecules in the size range of 8-20 nm. Aqueous dispersions of copolymers are investigated by DLS, cryogenic transmission electron microscopy, and atomic force microscopy, which show the formation of spherical aggregates with diameters of 60-350 nm, dependent on the arm number and length of star copolymers. The obtained sizes and size distributions, along with the hydroxyl-group-bearing cores, indicate that the star copolymer can be used as potential nanocarriers for drugs or bioimaging agents.

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Functional star polymers as reagents for efficient nucleic acids delivery into HT-1080 cells

Abstract: Fig. S1. Cryogenic transmission electron microscopy images obtained from PBS solutions of a) pDNA, b) STAR1.

Cited by 17 publications

References 38 publications

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

Selective Partial Hydrolysis of 2-isopropyl-2-oxazoline Copolymers towards Decreasing the Ability to Crystallize

Poly(oligoethylene glycol methacrylate) Star‐Shaped Copolymers with Hydroxypropyl Methacrylate Cores

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