Functional SNP in microRNA‐491‐5p binding site of MMP9 3′‐UTR affects cancer susceptibility

Pirooz, Homeira Javadi; Jafari, Niloofar; Rastegari, Mozhdeh; Fathi‐Roudsari, Mehrnoosh; Tasharrofi, Nooshin; Shokri, Gelareh; Tamadon, Mona; Sazegar, Hossein; Kouhkan, Fatemeh

doi:10.1002/jcb.26471

Cited by 34 publications

(25 citation statements)

References 43 publications

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“…MMP-9, a member of the MMP family, has been previously demonstrated to mediate trophoblast invasion and migration (32)(33)(34); for example, Rasstrigina et al (35) reported that the decreased expression levels of MMP-9 in the placenta contributed to the reduced invasiveness of trophoblasts. Notably, a previous study also observed that miR-491-5p suppressed the invasive ability of gastric, breast and lung cancer cells through targeting MMP-9 (36). Nonetheless, whether MMP-9 was implicated in the inhibitory effects of miR-491-5p on trophoblastic invasion remained relatively unknown.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑491‑5p inhibits trophoblast cell migration and invasion through targeting matrix metalloproteinase‑9 in preeclampsia

Liu

Zhou

et al. 2020

Mol Med Rep

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Insufficient invasion of trophoblasts is correlated with the development of preeclampsia (PE). MicroRNA (miR)-491-5p has been reported to be implicated in human cancer cell invasion; however, whether miR-491-5p is involved in the development of PE remains largely unclear. The aim of the present study was to investigate the role of miR-491-5p in trophoblastic invasion in vitro and to determine its underlying mechanism of action. The expression levels of miR-491-5p were validated using reverse transcription-quantitative PCR. The effects of miR-491-5p on trophoblast cell invasion were evaluated in vitro. Then, the association between miR-491-5p and its downstream target was investigated in both cell lines and clinical specimens. miR-491-5p expression levels were observed to be significantly increased in the placental tissues from patients with PE. The invasive capacity of HTR-8/SVneo trophoblast cells was suppressed following the upregulation of miR-491-5p and increased following the inhibition of miR-491-5p. Matrix metalloproteinase-9 (MMP-9), a well-known regulator of trophoblast cell invasion, was discovered to be a direct target of miR-491-5p in HTR-8/SVneo trophoblast cells. Moreover, miR-491-5p expression levels were found to be inversely correlated with MMP-9 expression levels in placental tissues from patients with PE. The overexpression of MMP-9 partly attenuated the inhibitory effects of miR-491-5p on HTR-8/SVneo trophoblast cells invasion. Collectively, these findings suggested that the aberrant expression of miR-491-5p may contribute to PE through suppressing trophoblast invasion, thus highlighting the novel roles of miR-491-5p in the molecular pathogenesis of PE. The present study also showed that the miR-491-5p/MMP-9 axis may be an effective biomarker or a viable drug target for therapeutic intervention in PE.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‑491‑5p inhibits trophoblast cell migration and invasion through targeting matrix metalloproteinase‑9 in preeclampsia

Liu

Zhou

et al. 2020

Mol Med Rep

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“…And also found the miRNAs expression changes in gastric cancer [11][12][13] . Recently discovered miRNA miR-491, is downregulated in several types of cancer, including breast cancer, oral squamous cell carcinoma, hepatocellular carcinoma, pancreatic, glioblastoma, ovarian cancer and hepatocellular carcinoma [13][14][15] . miR-491-5p has been shown to be a mature form of miR-491 that induce apoptosis and inhibit the proliferation of pancreatic, ovarian, breast cancer and colorectal cells, as well as the migration and invasion of glioma and breast, oral squamous cell.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-491-5p inhibit gastric cancer development by targeting EMT, cell adhesion genes and IFITM2

Wei¹,

Zhang

et al. 2020

Preprint

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Background: Gastric cancer (GC) is one of the deadliest cancers in China. And, it can be regulated by MicroRNAs (miRNAs) generally. miR-491-5p function as a tumor suppressor in different types of cancer, but we still don’t know the role of miR-491-5p in gastric cancer. Methods: Functional experiments including CCK-8 assay and transwell assay were performed. Furthermore, the underlying mechanism was explored through qRT-PCR and western blot assay. In addition, the function of miR-491-5p was also identified in vivo.Results: In this study, we found that high level of miR-491-5p caused a weak cell proliferation, migration and invasion abilities. In order to explore the role of miR-491-5p in vivo, we set a xenograft mouse model, and also found that high level of miR-491-5p suppressed tumor growth. Moreover, we found that miR-491-5p regulate the tumor development thought regulate the expression of EMT(Epithelial-mesenchymal transition), cell adhesion genes and IFITM2. Conclusions: These data show that miR-491-5p function as a tumor suppressor in GC both in vitro and in vivo.

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“…SNPs in miRNA binding sites have been associated with cancer risk and outcomes. Alterations in these sites could modify the binding sites or change the binding affinity, leading to escape from miRNA regulation [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

The association between rs16917496 T/C polymorphism ofSET8gene and cancer risk in Asian populations: a meta-analysis

et al. 2018

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Epidemiological studies have demonstrated close associations between SET8 rs16917496 T/C polymorphism and cancer risk, but the results of published studies were not consistent. We therefore performed this meta-analysis to explore the associations between rs16917496 T/C polymorphism and cancer risk. Five online databases were searched. Odds ratios (ORs) with a 95% confidence interval (CI) were calculated to assess the association between rs16917496 T/C polymorphism and cancer risk. In addition, heterogeneity, accumulative, sensitivity analysis, and publication bias were conducted to check the statistical power. Overall, 13 publications involving 5878 subjects were identified according to included criteria. No significant cancer risk was observed in genetic model of SET8 rs16917496 T/C polymorphism in Asian populations (C vs. T: OR = 1.04, 95%CI = 0.88–1.23, P = 0.63%; TC vs. TT: OR = 1.17, 95%CI = 0.96–1.24, P = 0.11%; CC vs. TT: OR = 0.90, 95%CI = 0.60–1.37, P = 0.63; TC+CC vs. TT: OR = 1.11, 95%CI = 0.90–1.38, P = 0.33; CC vs. TT+TC: OR = 0.92, 95%CI = 0.65–1.30, P = 0.63). Furthermore, similar associations were found in the subgroup analysis of race diversity, control design, genotyping methods, and different cancer types. In summary, our meta-analysis indicated that the SET8 rs16917496 T/C polymorphism may not play a critical role in cancer development in Asian populations.

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Functional SNP in microRNA‐491‐5p binding site of MMP9 3′‐UTR affects cancer susceptibility

Cited by 34 publications

References 43 publications

MicroRNA‑491‑5p inhibits trophoblast cell migration and invasion through targeting matrix metalloproteinase‑9 in preeclampsia

MicroRNA‑491‑5p inhibits trophoblast cell migration and invasion through targeting matrix metalloproteinase‑9 in preeclampsia

MicroRNA-491-5p inhibit gastric cancer development by targeting EMT, cell adhesion genes and IFITM2

The association between rs16917496 T/C polymorphism ofSET8gene and cancer risk in Asian populations: a meta-analysis

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