Functional significance of cumulus expansion in the mouse: Roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass

Chen, L.; Russell, Paul T.; Larsen, William J.

doi:10.1002/mrd.1080340114

Cited by 269 publications

(182 citation statements)

References 26 publications

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“…1). They include crucial genes reported to be essential for ECM formation and oocyte competence like TNFAIP6, EREG, sprouty homolog 2 (SPRY2), PGR, and hyaluronan synthase 2 (HAS2; Chen et al 1993, Fulop et al 2003, Ochsner et al 2003a, Cillo et al 2007.…”

Section: Cellular and Molecular Function Analysismentioning

confidence: 99%

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Assidi¹,

Dieleman²,

Sirard³

2010

Reproduction

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Cumulus cells (CCs) are essential for oocytes to reach full development competency and become fertilized. Many major functional properties of CCs are triggered by gonadotropins and governed by the oocyte. Consequently, cumulus may reflect oocyte quality and is often used for oocyte selection. The most visible function of CCs is their ability for rapid extracellular matrix expansion after the LH surge. Although unexplained, LH induces the final maturation and improves oocyte quality. To study the LH signaling and gene expression cascade patterns close to the germinal vesicle breakdown, bovine CCs collected at 2 h before and 6 h after the LH surge were hybridized to a custom-made microarray to better understand the LH genomic action and find differentially expressed genes associated with the LH-induced oocyte final maturation. Functional genomic analysis of the 141 overexpressed and 161 underexpressed clones was performed according to their molecular functions, gene networks, and cell compartments. Following real-time PCR validation of our gene lists, some interesting pathways associated with the LH genomic action on CCs and their possible roles in oocyte final maturation, ovulation, and fertilization are discussed. A list of early potential markers of oocyte competency in vivo and in vitro is thereafter suggested. These early biomarkers are a preamble to understand the LH molecular pathways that trigger the final oocyte competence acquisition process in bovine.

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Section: Cellular and Molecular Function Analysismentioning

confidence: 99%

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Assidi¹,

Dieleman²,

Sirard³

2010

Reproduction

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“…During our IVF experiments, we noted that Ptger2 Ϫ/Ϫ COCs were more sticky and more resistant to hyaluronidase treatment compared with WT COCs. Hyaluronan is a major component of the cumulus ECM, and production of hyaluronan induces cumulus expansion (22). Sperm use their own hyaluronidase to penetrate the cumulus ECM layer.…”

Section: Integrin-dependent Hyaluronidase Resistance Causes Fertilizamentioning

confidence: 99%

Timely interaction between prostaglandin and chemokine signaling is a prerequisite for successful fertilization

Tamba¹,

Yodoi²,

Segi-Nishida

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Timely interaction between the egg and sperm is required for successful fertilization; however, little is known about the signaling therein. Prostaglandin (PG) E receptor EP2-deficient (Ptger2 ؊/؊ ) female mice exhibit a severe fertilization defect. We investigated the molecular events leading to this failure. We found increased gene expression for chemokines, such as Ccl2, Ccl7, and Ccl9, in Ptger2 ؊/؊ cumulus cells (the somatic cells surrounding the egg) compared with wild-type cells. Furthermore, under physiological conditions, cumulus-derived chemokine signaling was found to have a dual action; CCL7 facilitates sperm migration to the cumulus-egg complex and integrin-mediated cumulus extracellular matrix (ECM) assembly to protect eggs. However, in the absence of PGE 2-EP2 signaling, chronic CCL7 signaling results in excessive integrin engagement to the ECM, making the cumulus ECM resistant to sperm hyaluronidase, thereby preventing sperm penetration. Our findings indicate that PGE 2-EP2 signaling negatively regulates the autocrine action of chemokines and prevents excessive cumulus ECM assembly. This interaction between PG and chemokine signaling is required for successful fertilization.O vulation and fertilization are key processes in mammalian female reproduction, which are strictly regulated by pituitary gonadotropins, follicle-stimulating hormone, and luteinizing hormone. These hormones induce various preovulatory processes, including follicular development, oocyte maturation, and cumulus expansion, and trigger ovulation (1). Ovulated eggs move to the oviduct; subsequently the timely interaction between an egg and sperm leads to successful fertilization (2). Whereas various molecules are known to regulate preovulatory processes locally in the follicle under the control of gonadotropin (3), little is known about the regulation of postovulatory processes, including fertilization in the oviduct, which gonadotropin does not control.One potential candidate for the local mediators regulating fertilization within the oviduct is chemokines. Chemokines constitute a family of structurally related, inducible peptides involved in various immune responses and inflammation, acting as chemoattractants and activators of specific types of leukocytes (4). Approximately 40 chemokines, grouped into distinct families and secreted by various cells, are currently known (5). It was recently reported that in humans, CCL5 (also called RANTES) is present in the follicular fluid and stimulates the in vitro migration of sperm, which express CCR1, a receptor for CCL5 (6). Based on these findings, it has been proposed that chemokines may participate in sperm guidance within the genital tract (7). The physiological significance of such chemokine signaling within the oviduct remains elusive, however.The cumulus oophorus is composed of a group of closely associated granulosa cells that surround the oocyte in the antral follicle and are collectively called cumulus cells (8). In response to a surge in luteinizing hormone, the cumulus cells ...

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“…Furthermore, the HABP2 gene is another candidate gene for NBA and plays a role in the integrity of mouse cumulus extracellular matrix (Chen et al . 1993). …”

mentioning

confidence: 99%

Genome‐wide association study for reproductive traits in a Large White pig population

et al. 2018

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SummaryUsing the PorcineSNP80 BeadChip, we performed a genome‐wide association study for seven reproductive traits, including total number born, number born alive, litter birth weight, average birth weight, gestation length, age at first service and age at first farrowing, in a population of 1207 Large White pigs. In total, we detected 12 genome‐wide significant and 41 suggestive significant SNPs associated with six reproductive traits. The proportion of phenotypic variance explained by all significant SNPs for each trait ranged from 4.46% (number born alive) to 11.49% (gestation length). Among them, 29 significant SNPs were located within known QTL regions for swine reproductive traits, such as corpus luteum number, stillborn number and litter size, of which one QTL region associated with litter size contained the ALGA0098819 SNP for total number born. Subsequently, we found that 376 functional genes contained or were near these significant SNPs. Of these, 14 genes—BHLHA15, OCM2, IL1B2, GCK, SMAD2, HABP2, PAQR5, GRB10, PRELID2, DMKN, GPI, GPIHBP1, ADCY2 and ACVR2B—were considered important candidates for swine reproductive traits based on their critical roles in embryonic development, energy metabolism and growth development. Our findings contribute to the understanding of the genetic mechanisms for reproductive traits and could have a positive effect on pig breeding programs.

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Functional significance of cumulus expansion in the mouse: Roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass

Cited by 269 publications

References 26 publications

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Cumulus cell gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence

Timely interaction between prostaglandin and chemokine signaling is a prerequisite for successful fertilization

Genome‐wide association study for reproductive traits in a Large White pig population

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