Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein

Tada, Yuichi; Kawano, Ryuichi; Komatsubara, Shiho; Nishimura, Hideki; Katsuhara, Maki; Ozaki, Soichi; Terashima, Shin; Yano, Kentarô; Endo, Chikara; Sato, Muneo; Okamoto, Mami; Sawada, Yuji; Hirai, Masami Yokota; Kurusu, Takamitsu

doi:10.1016/j.plantsci.2018.10.019

Cited by 20 publications

(11 citation statements)

References 54 publications

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“…Os04g0107600 and Os09g0543400 encode arginine decarboxylase 2 and ornithine decarboxylase, respectively, and are able to modulate the amount of polyamine [40]. Glutathione, carotenoids, anthocyanins, and flavonoids are important antioxidants and might help to eliminate the excessive active oxygen that is produced under abiotic stresses [41][42][43][44][45]. Unsaturated fatty acids, fatty acids, and proline act as important osmoprotectants in response to drought stress [41,43,44,46].…”

Section: Transcriptome Analysis Of the Transgenic Lines And Wt Under Osmotic Stressmentioning

confidence: 99%

Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

Wang

et al. 2021

IJMS

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Gibberellin 2-oxidase (GA2ox) plays an important role in the GA catabolic pathway and the molecular function of the OsGA2ox genes in plant abiotic stress tolerance remains largely unknown. In this study, we functionally characterized the rice gibberellin 2-oxidase 8 (OsGA2ox8) gene. The OsGA2ox8 protein was localized in the nucleus, cell membrane, and cytoplasm, and was induced in response to various abiotic stresses and phytohormones. The overexpression of OsGA2ox8 significantly enhanced the osmotic stress tolerance of transgenic rice plants by increasing the number of osmotic regulators and antioxidants. OsGA2ox8 was differentially expressed in the shoots and roots to cope with osmotic stress. The plants overexpressing OsGA2ox8 showed reduced lengths of shoots and roots at the seedling stage, but no difference in plant height at the heading stage was observed, which may be due to the interaction of OsGA2ox8 and OsGA20ox1, implying a complex feedback regulation between GA biosynthesis and metabolism in rice. Importantly, OsGA2ox8 was able to indirectly regulate several genes associated with the anthocyanin and flavonoid biosynthetic pathway and the jasmonic acid (JA) and abscisic acid (ABA) biosynthetic pathway, and overexpression of OsGA2ox8 activated JA signal transduction by inhibiting the expression of jasmonate ZIM domain-containing proteins. These results provide a basis for a future understanding of the networks and respective phenotypic effects associated with OsGA2ox8.

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Section: Transcriptome Analysis Of the Transgenic Lines And Wt Under Osmotic Stressmentioning

confidence: 99%

Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

Wang

et al. 2021

IJMS

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“…Light treatment could prevent the decrease in ascorbic acid and maintain the content of soluble sugars in Chinese kale during storage (Noichinda et al., 2007). Pretreatment with hot water or UV‐C reduces the rapid weight loss and delays the yellowing of Chinese kale (Tada et al., 2019). These postharvest treatments tend to involve friendly methods or techniques for extending the shelf‐life of vegetables.…”

Section: Introductionmentioning

confidence: 99%

High oxygen atmospheric packaging treatment regulates the postharvest changes of Chinese kale (Brassica oleracea var. alboglabra) during storage

Wang

Huang

et al. 2021

Journal of Food Science

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Chinese kale is one of the most popular vegetables in southern China and Asia, but it has a short shelf-life. The effect of high oxygen atmospheric packaging (HOAP) treatment on the respiration rate as well as chlorophyll content and the expression of their metabolism-related genes of the soluble proteins in Chinese kale during storage were assessed. The results showed that Chinese kale subjected to HOAP treatment showed stimulated respiration rate and regulated expression of chlorophyll metabolism-related genes, such as BrChlases, BrPPH (pheophytin pheophorbide hydrolase), BrPAO (pheidea oxygenase gene), BrRCCR (red chlorophyll catabolite reductase), and BrSAG12 (senescenceassociated gene), compared to the Chinese kale in the control. The activities of chlorophyll enzymes, that is, Chlase and Mg-dechelatase, were also influenced by HOAP treatment during storage. Furthermore, the total content of soluble proteins was stimulated to accumulate, and the intensity of protein bands, detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling, increased in HOAP-treated samples. Based on the current results, as well as the results of our previous study regarding HOAP treatment of other vegetables, we speculate that HOAP may function by regulating the respiration rate and the accumulation of functional proteins, especially chlorophyll catabolic and antioxidant enzymes, to maintain the freshness of Chinese kale during storage. Practical Application: HOAP treatment could be a potential method for delaying quality changes and extending the shelf-life of Chinese kale after harvest.

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“…Transgenic Arabidopsis plants expressing S. virginicus cDNAs have revealed upregulation of polyol and downregulation of glucosinolate and indole acetic acid biosynthesis/metabolic pathways. In addition, in these transgenic plants, the increase in 3-aminopropanoic acid, citramalic acid, and isocitric acid concentrations proved to be associated with enhanced salt tolerance (Tada et al 2019). On the other hand, the tolerable NaCl concentration and osmotic adjustment reportedly depends on the S. virginicus genotype.…”

Section: Psammophyte Speciesmentioning

confidence: 98%

“…S. virginicus can accumulate Na + , Cl À , and K + while maintaining a high K + /Na + ratio under strong salinity. In addition, in some genotypes, proline accumulation has been found in the shoots (Tada et al 2019). According to lipid content, this plant could be a potential source of biofuel, producing abundant biomass in saltaffected land (Patel et al 2019).…”

Section: Psammophyte Speciesmentioning

confidence: 99%

Ecophysiology and Uses of Halophytes in Diverse Habitats

Bueno

Cordovilla

2020

Handbook of Halophytes

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Climate change is decreasing global crop production and altering the balance of ecosystems worldwide, making it urgent to find alternative species that are not only resistant to drought and environmental salinity but also capable of developing in a great diversity of habitats in order to ease pressure on cropping systems and restore lands degraded by salt. Halophytes constitute a group of plants that can fulfill these demands, thanks their morphological, anatomical, physiological, biochemical, and genetic adaptations that enable them to survive in a wide variety of habitats (wetlands, deserts, or tropical and temperate zones). In this chapter, we summarize recently discovered ecophysiological mechanisms in a total of 19 halophytes divided according their respective habitats: xerohalophytes, psammophytes, and hydrohalophytes. We highlight adaptations that enable these plants to survive under stress conditions using many strategies, including phenotypic plasticity, salt excretion (salt glands, trichomes), saline dilution (succulent leaves), growth modulation (increased root/shoot ratio), stomatic and CO 2 resistance, tight transpiration control, water-use efficiency, Na + compartmentalization in the vacuole (through the Na + /H + antiporter of tonoplast), osmolyte

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Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein

Cited by 20 publications

References 54 publications

Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

High oxygen atmospheric packaging treatment regulates the postharvest changes of Chinese kale (Brassica oleracea var. alboglabra) during storage

Ecophysiology and Uses of Halophytes in Diverse Habitats

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