Functional screen for microRNAs of Nilaparvata lugens reveals that targeting of glutamine synthase by miR-4868b regulates fecundity

Fu, Xian; Li, Tengchao; Chen, Jie; Dong, Yi; Qiu, Jinbo; Kang, Kui Dong; Zhang, Wenqing

doi:10.1016/j.jinsphys.2015.11.003

Cited by 23 publications

(22 citation statements)

References 65 publications

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“…For instance, in Aedes aegypti , the w Melpop-CLA strain of Wolbachia can alter aae-miR-2940 to increase metalloprotease gene expression for self-maintenance in mosquito hosts [30]. In insects, many lines of evidence revealed that miRNAs could operate in ovarian development [36–40]. Here, we reported for the first time that Wolbachia could modulate miRNAs of host ovaries.…”

Section: Discussionmentioning

confidence: 82%

How do Wolbachia modify the Drosophila ovary? New evidences support the “titration-restitution” model for the mechanisms of Wolbachia-induced CI

He¹,

Zheng²,

Yu³

et al. 2019

BMC Genomics

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Background Cytoplasmic incompatibility (CI) is the most common phenotype induced by endosymbiont Wolbachia and results in embryonic lethality when Wolbachia -modified sperm fertilize eggs without Wolbachia . However, eggs carrying the same strain of Wolbachia can rescue this embryonic death, thus producing viable Wolbachia -infected offspring. Hence Wolbachia can be transmitted mainly by hosts’ eggs. One of the models explaining CI is “titration-restitution”, which hypothesized that Wolbachia titrated-out some factors from the sperm and the Wolbachia in the egg would restitute the factors after fertilization. However, how infected eggs rescue CI and how hosts’ eggs ensure the proliferation and transmission of Wolbachia are not well understood. Results By RNA-seq analyses, we first compared the transcription profiles of Drosophila melanogaster adult ovaries with and without the w Mel Wolbachia and identified 149 differentially expressed genes (DEGs), of which 116 genes were upregulated and 33 were downregulated by Wolbachia infection. To confirm the results obtained from RNA-seq and to screen genes potentially associated with reproduction, 15 DEGs were selected for quantitative RT-PCR (qRT-PCR). Thirteen genes showed the same changing trend as RNA-seq analyses. To test whether these genes are associated with CI, we also detected their expression levels in testes. Nine of them exhibited different changing trends in testes from those in ovaries. To investigate how these DEGs were regulated, sRNA sequencing was performed and identified seven microRNAs (miRNAs) that were all upregulated in fly ovaries by Wolbachia infection. Matching of miRNA and mRNA data showed that these seven miRNAs regulated 15 DEGs. Wolbachia -responsive genes in fly ovaries were involved in biological processes including metabolism, transportation, oxidation-reduction, immunity, and development. Conclusions Comparisons of mRNA and miRNA data from fly ovaries revealed 149 mRNAs and seven miRNAs that exhibit significant changes in expression due to Wolbachia infection. Notably, most of the DEGs showed variation in opposite directions in ovaries versus testes in the presence of Wolbachia , which generally supports the “titration-restitution” model for CI. Furthermore, genes related to metabolism were upregulated, which may benefit maximum proliferation and transmission of Wolbachia . This provides new insights into the molecular mechanisms of Wolbachia -induced CI and ...

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Section: Discussionmentioning

confidence: 82%

How do Wolbachia modify the Drosophila ovary? New evidences support the “titration-restitution” model for the mechanisms of Wolbachia-induced CI

He¹,

Zheng²,

Yu³

et al. 2019

BMC Genomics

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“…Glutamine affords nitrogen sources for the biosynthesis of amino acids, pyrimidines and purines. Glutamine synthase (GS) catalyses the conversation of glutamate to glutamine (Gln) with Gln and GS playing some important roles in the fecundity of insects (Fu et al ., ; Zhai et al ., , ). In this study, the expression of the GS gene followed the same trend as the TPS gene (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants

Liu

Hao

et al. 2017

Plant Biotechnology Journal

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SummaryBrown planthopper (Nilaparvata lugens Stål, BPH) causes huge economic losses in rice‐growing regions, and new strategies for combating BPH are required. To understand how BPHs respond towards BPH‐resistant plants, we systematically analysed the metabolic differences between BPHs feeding on the resistant and susceptible plants using NMR and GC‐FID/MS. We also measured the expression of some related genes involving glycolysis and biosyntheses of trehalose, amino acids, chitin and fatty acids using real‐time PCR. BPH metabonome was dominated by more than 60 metabolites including fatty acids, amino acids, carbohydrates, nucleosides/nucleotides and TCA cycle intermediates. After initial 12 h, BPHs feeding on the resistant plants had lower levels of amino acids, glucose, fatty acids and TCA cycle intermediates than on the susceptible ones. The levels of these metabolites recovered after 24 h feeding. This accompanied with increased level in trehalose, choline metabolites and nucleosides/nucleotides compared with BPH feeding on the susceptible plants. Decreased levels of BPH metabolites at the early feeding probably resulted from less BPH uptakes of sap from resistant plants and recovery of BPH metabolites at the later stage probably resulted from their adaptation to the adverse environment with their increased hopping frequency to ingest more sap together with contributions from yeast‐like symbionts in BPHs. Throughout 96 h, BPH feeding on the resistant plants showed significant up‐regulation of chitin synthase catalysing biosynthesis of chitin for insect exoskeleton, peritrophic membrane lining gut and tracheae. These findings provided useful metabolic information for understanding the BPH–rice interactions and perhaps for developing new BPH‐combating strategies.

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“…The total proteins used for the detection were isolated as previously described . For Western blotting, the crude insect total protein (40 µg) sample was separated on SDS‐PAGE gel (8%) and transferred to PVDF membranes (0.45 µm; Millipore, Billerica, MA).…”

Section: Methodsmentioning

confidence: 99%

MicroRNA and dsRNA targeting chitin synthase A reveal a great potential for pest management of the hemipteran insectNilaparvata lugens

Chen

Fan

et al. 2017

Pest. Manag. Sci.

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Functional screen for microRNAs of Nilaparvata lugens reveals that targeting of glutamine synthase by miR-4868b regulates fecundity

Cited by 23 publications

References 65 publications

How do Wolbachia modify the Drosophila ovary? New evidences support the “titration-restitution” model for the mechanisms of Wolbachia-induced CI

How do Wolbachia modify the Drosophila ovary? New evidences support the “titration-restitution” model for the mechanisms of Wolbachia-induced CI

Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants

MicroRNA and dsRNA targeting chitin synthase A reveal a great potential for pest management of the hemipteran insectNilaparvata lugens

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