Functional roles of miR-625-5p and miR-874-3p in the progression of castration resistant prostate cancer

Aktan, Çağdaş; Çal, Çağ; Kaymaz, Burçin Tezcanlı; Günel, Nur Selvi; Kıpçak, Sezgi; Özel, Buket; Gündüz, Cumhur; Küçükaslan, Ali Şahin; Jafari, Duygu Aygunes; Kosova, Buket

doi:10.1016/j.lfs.2022.120603

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…Through Starbase, an online target gene prediction website, we preliminarily screened the downstream potential target genes of LINC00958, among which miR-625-5p attracted our attention. Located on human chromosome 14q23.3, this gene was first found to have an aberrant expression in the whole genome of cervical cancer and was subsequently confirmed to be of important potential significance in urogenital tumors such as prostate cancer and endometrial cancer [6][7][8]. Although the direct relationship between miR-625-5p and BC has not been verified yet, some studies have found that miR-625-5p can form a feedback channel with Runx1t1/TCF4 and promote BC progression under the action of RBM24 [9], which also preliminarily reveals the possible close relationship between miR-625-5p and the occurrence and development of BC.…”

Section: Introductionmentioning

confidence: 99%

LINC00958 Inhibits Autophagy of Bladder Cancer Cells via Sponge Adsorption of miR-625-5p to Promote Tumor Angiogenesis and Oxidative Stress

Xiao

Wang

Cheng

et al. 2022

Oxidative Medicine and Cellular Longevity

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Objective. This study further explored LINC00958’s role in promoting tumor angiogenesis (AG) and oxidative stress (OS) development by inhibiting BC cell autophagy through sponge adsorption of miR-625-5p. Methods. BC patients and healthy controls who visited our hospital between June 2017 and February 2019 were selected as the research group (RG) and the control group (CG), respectively, with a total of 133 study subjects. Peripheral blood LINC00958 and miR-625-5p in both cohorts of participants were detected. Additionally, human bladder transitional cell carcinoma cells (T24 and J82) and human normal urothelial cells (SV-HUC-1) were purchased. Alterations in cell biological behavior were observed after transfecting miR-625-5p-mimics, miR-625-5p-inhibition, and miR-625-5p-NC sequences into these cells, respectively. Besides, ELISA was performed to quantify inflammatory factors (IFs), AG indicators, and OS indexes in cells. Subsequently, a double luciferase reporter (DLR) assay was performed to verify the targeting relationship between LINC00958 and miR-625-5p. Finally, BALB/c-nu nude mice were purchased, and T24 cells transfected with silenced LINC00958 and miR-625-5p expression sequences were used to establish subcutaneous tumors to observe tumor growth and pathological changes. Results. RG exhibited higher LINC00958 and lower miR-625-5p than CG. LINC00958 and miR-625-5p were strongly linked to myometrial invasion (MI), lymph node metastasis (LNM), distant metastasis (DM), and histology in BC patients, and the increase of LINC00958 and the decrease of miR-625-5p predicted an increased risk of prognostic death in such patients. After miR-625-5p inhibition, the capacity of BC cells to proliferate, invade, and migrate enhanced and the AG, inflammatory response, and OS injury increased, while the apoptosis rate and autophagy ability decreased. The DLR assay revealed inhibited LINC00958WT fluorescence activity by miR-625-5p-mimics, while the biological behavior of BC cells cotransfected with sh-LINC00958 and miR-625-5p-inhibition had no difference with the functions of sh-control and miR-625-5p-NC cotransfected cells. Finally, the nude mouse tumorigenesis experiment showed that the tumor mass, volume, and histopathological features of the sh-LINC00958 group were decreased compared with the sh-control group, while those of the miR-625-5p-inhibition group were increased versus miR-625-5p-NC. Conclusions. In BC, LINC00958 is highly expressed while miR-625-5p is underexpressed. LINC00958 can inhibit cell autophagy to enhance cell activity; promote OS, inflammation, and AG; and regulate tumor immunity by targeting miR-625-5p, thus participating in the development of BC.

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Section: Introductionmentioning

confidence: 99%

LINC00958 Inhibits Autophagy of Bladder Cancer Cells via Sponge Adsorption of miR-625-5p to Promote Tumor Angiogenesis and Oxidative Stress

Xiao

Wang

Cheng

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Çağdaş Aktan et al evaluated miR-625-5p and miR-874-3p upregulation in ADPC to CRPC transition in LNCaP-104R2 and LNCaP-Abl cell lines. Suppression of miR-625-5p or miR-874-3p resulted in decreased proliferation of CRPC cells [48]. Jani Silva et al designed a study to evaluate the prognostic potential of nine miRNAs in the liquid biopsies (plasma) of mCRPC patients treated with second-generation androgen receptor axis-targeted (ARAT) agents, abiraterone acetate (AbA) and enzalutamide (ENZ).…”

Section: Micrornasmentioning

confidence: 99%

Research Progress on the Mechanism of Androgen Receptor Signaling Pathway in Castration-Resistant Prostate Cancer

Cui,

2023

ann urol oncol

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Prostate cancer (Pca) remains the most common malignancy worldwide in men, and the second leading cause of mortality only to lung cancer. Besides surgery, androgen deprivation therapy (ADT) is a major treatment for Pca. However, ADT leads to the inevitable progression of castration-resistant Pca (CRPC). The transition from hormone-dependent Pca (ADPC) to CRPC has been shown to involve reactivation of the androgen receptor (AR) signaling pathway. The evidence become strong that Pca develop adaptive mechanisms for maintaining AR signaling to allow for survival and further evolution. This article mainly reviews the research progress of the mechanism(s) of AR signaling in CRPC and provides scientific basis and new ideas for the diagnosis and treatment of this phenotype.

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Clinical significance of miR-625-5p in patients with sepsis-induced acute kidney injury based on bioinformatics analysis

Gong,

Chen,

et al. 2024

Int Urol Nephrol

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Functional roles of miR-625-5p and miR-874-3p in the progression of castration resistant prostate cancer

Cited by 4 publications

References 32 publications

LINC00958 Inhibits Autophagy of Bladder Cancer Cells via Sponge Adsorption of miR-625-5p to Promote Tumor Angiogenesis and Oxidative Stress

LINC00958 Inhibits Autophagy of Bladder Cancer Cells via Sponge Adsorption of miR-625-5p to Promote Tumor Angiogenesis and Oxidative Stress

Research Progress on the Mechanism of Androgen Receptor Signaling Pathway in Castration-Resistant Prostate Cancer

Clinical significance of miR-625-5p in patients with sepsis-induced acute kidney injury based on bioinformatics analysis

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