Functional role of polyhydroxy compounds on protein structure and thermal stability studied by circular dichroism spectroscopy

Ortbauer, Martina; Popp, Marianne

doi:10.1016/j.plaphy.2008.02.002

Cited by 22 publications

(24 citation statements)

References 34 publications

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“…Far-UV CD spectrum of the native GA (pH 7.0, 25°C) has also been included in Figure 5 for comparison. As can be seen from the figure, far-UV CD spectrum of the TD state of GA showed ~48% loss in the MRE 222 nm value compared to the native GA (Tables 1 and 4), along with a shift in the CD spectral signals, suggesting denaturation of GA at high temperature as observed with other proteins [30, 36, 40]. Interestingly, addition of GLC or TRE to the TD state of GA markedly increased the MRE 222 nm value by ~110% and ~91%, respectively (Table 4), showing significant reversal in the CD spectral characteristics close to the native GA.…”

Section: Resultsmentioning

confidence: 54%

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Zaroog

Kadir

Tayyab

2013

The Scientific World Journal

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Different spectral probes were employed to study the stabilizing effect of various polyols, such as, ethylene glycol (EG), glycerol (GLY), glucose (GLC) and trehalose (TRE) on the native (N), the acid-denatured (AD) and the thermal-denatured (TD) states of Aspergillus niger glucoamylase (GA). Polyols induced both secondary and tertiary structural changes in the AD state of enzyme as reflected from altered circular dichroism (CD), tryptophan (Trp), and 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence characteristics. Thermodynamic analysis of the thermal denaturation curve of native GA suggested significant increase in enzyme stability in the presence of GLC, TRE, and GLY (in decreasing order) while EG destabilized it. Furthermore, CD and fluorescence characteristics of the TD state at 71°C in the presence of polyols showed greater effectiveness of both GLC and TRE in inducing native-like secondary and tertiary structures compared to GLY and EG.

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Section: Resultsmentioning

confidence: 54%

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Zaroog

Kadir

Tayyab

2013

The Scientific World Journal

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“…Some polyols are thought to possess protective properties towards protein structures, membranes and liposomes (Ortbauer and Popp 2008) through several modes of function. As discussed above, concentrations of many polyols do not significantly fluctuate in the short term, leading to suggestions that this improves their ability function in stabilising cellular structures (e.g.…”

Section: Polyol Chemistry and Cellular Functionsmentioning

confidence: 99%

Polyols as biomarkers and bioindicators for 21st century plant breeding

Merchant

Richter

2011

Functional Plant Biol.

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Abstract. Characterising changes in the plant metabolome is central to understanding adaptive responses to environmental change. New and improved quantitative and qualitative technologies have enabled the characterisation of plant metabolism at unprecedented scales and precision. New frontiers have therefore emerged for improving our understanding of the adaptability of plant metabolic networks. However, despite these advances, outcomes for 'in field' plant management remain largely based on subsets of plant metabolism due to broader scale network complexity. The synthesis and occurrence of polyols offer considerable promise as bioindicators of plant health and biomarkers for use as selective traits for plant improvement. Polyols are polyohydroxy compounds that may be either open chain (acyclic) alditols or cyclic compounds (cyclohexan-hexols), usually termed cyclitols or inositols. Here we highlight the functions of polyols in stress acclimation or amelioration and as sinks for carbon and indicate their potential for the development of integrated measures of plant function using new technologies in 21st century plant breeding.

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“…Such reports support the hypothesis that the biosynthesis of this cyclitol is distributed in the chromophyte line as proposed by Fujii et al 1995 and Bangiophyceae (Rhodophyta). Conduritol (1,4 ⁄ 2,3 cyclohexanetetrol), another cyclitol accumulated during saline stress in higher plants, seems to be species-specific even in the plant kingdom (Ortbauer and Popp 2008). Because 1,4 ⁄ 2,5 cyclohexanetetrol is present in a broader group of algal species, maybe it would be a better osmoprotectant than conduritol.…”

Section: Discussionmentioning

confidence: 99%

NITZSCHIA OVALIS(BACILLARIOPHYCEAE) MONO LAKE STRAIN ACCUMULATES 1,4/2,5 CYCLOHEXANETETROL IN RESPONSE TO INCREASED SALINITY¹

Garza-Sánchez

Chapman

Cooper

2009

Journal of Phycology

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The growth of microalgae in hypersaline conditions requires that cells accumulate osmoprotectants. In many instances, these are polyols. We isolated the diatom Nitzschia ovalis H. J. Arn. from the saline and alkaline water body Mono Lake (CA, USA). This isolate can grow in salinities ranging from 5 to 120 parts per thousand (ppt) of salt but normally at 90 ppt salinity. In this report, we identified the major polyol osmoprotectant as 1,4/2,5 cyclohexanetetrol by electron ionization-mass spectrometry (EI-MS), (1) H, (13) C nuclear magnetic resonance spectroscopy (NMR), and infrared (IR) and showed an increase in cellular concentration in response to rising salinity. This increase in the cyclitol concentration was evaluated by gas chromatography of the derived tetraacetylated cyclohexanetetrol obtaining an average of 0.7 fmol · cell(-1) at 5 ppt and rising to 22.5 fmol · cell(-1) at 120 ppt. The 1,4/2,5 cyclohexanetetrol was also detected in the red alga Porphyridium purpureum. Analysis of the free amino acid content in N. ovalis cultures exposed to changes in salinity showed that proline and lysine also accumulate with increased salinity, but the cellular concentration of these amino acids is about 10-fold lower than the concentration of 1,4/2,5 cyclohexanetetrol. The comparison of amino acid concentration per cell with cyclitol suggests that this polyol is important in compensating the cellular osmotic pressure due to increased salinity, but other physiological functions could also be considered.

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Functional role of polyhydroxy compounds on protein structure and thermal stability studied by circular dichroism spectroscopy

Cited by 22 publications

References 34 publications

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Polyols as biomarkers and bioindicators for 21st century plant breeding

NITZSCHIA OVALIS(BACILLARIOPHYCEAE) MONO LAKE STRAIN ACCUMULATES 1,4/2,5 CYCLOHEXANETETROL IN RESPONSE TO INCREASED SALINITY¹

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Functional role of polyhydroxy compounds on protein structure and thermal stability studied by circular dichroism spectroscopy

Cited by 22 publications

References 34 publications

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase

Polyols as biomarkers and bioindicators for 21st century plant breeding

NITZSCHIA OVALIS(BACILLARIOPHYCEAE) MONO LAKE STRAIN ACCUMULATES 1,4/2,5 CYCLOHEXANETETROL IN RESPONSE TO INCREASED SALINITY1

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NITZSCHIA OVALIS(BACILLARIOPHYCEAE) MONO LAKE STRAIN ACCUMULATES 1,4/2,5 CYCLOHEXANETETROL IN RESPONSE TO INCREASED SALINITY¹