Functional role of miRNA-34A in smooth muscle cell functions and neointima formation

Yang, Feng; Chen, Q.; Wen, G.; Zhang, C.; Luong, Le Anh; Zhu, Jiang; Zhang, L.; Xiao, Qingzhong

doi:10.1016/j.atherosclerosis.2015.10.060

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The VSMC phenotype is influenced by signals from the local environment, including miRNAs. To date, several studies have demonstrated that miRNAs are pleiotropic modulators of VSMC differentiation [18][19][20][21]. The molecular mechanisms underlying the regulation of VSMC differentiation by these miRNAs are diverse, but mainly involve the direct or indirect regulation of target gene expression.…”

Section: Discussionmentioning

confidence: 99%

The Interaction between 30b-5p miRNA and MBNL1 mRNA is Involved in Vascular Smooth Muscle Cell Differentiation in Patients with Coronary Atherosclerosis

Woo

Liu

Lin

et al. 2019

IJMS

View full text Add to dashboard Cite

Vascular smooth muscle cells (VSMCs) in the arterial wall have diverse functions. In pathological states, the interplay between transcripts and microRNAs (miRNAs) leads to phenotypic changes. Understanding the regulatory role of miRNAs and their target genes may reveal how VSMCs modulate the pathogenesis of coronary artery disease. Laser capture microdissection was performed on aortic wall tissues obtained from coronary artery bypass graft patients with and without recent acute myocardial infarction (MI). The mSMRT-qPCR miRNA assay platform (MiRXES, Singapore) was used to profile miRNA. The miRNA data were co-analyzed with significant mRNA transcripts. TargetScan 7.1 was applied to evaluate miRNA–mRNA interactions. The miRNA profiles of 29 patients (16 MI and 13 non-MI) were evaluated. Thirteen VSMC-related miRNAs were differentially expressed between the MI and non-MI groups. Analysis revealed seven miRNA-targeted mRNAs related to muscular tissue differentiation and proliferation. TargetScan revealed that among the VSMC-related transcripts, MBNL1 had a recognition site that matched the hsa-miR-30b-5p target seed sequence. In addition to predicted analysis, our experiment in vitro with human VSMC culture confirmed that hsa-miR-30b-5p negatively correlated with MBNL1. Our data showed that overexpression of hsa-miR-30b-5p led to downregulation of MBNL1 in VSMCs. This process influences VSMC proliferation and might be involved in VSMC differentiation.

show abstract

Section: Discussionmentioning

confidence: 99%