Functional role of antibody against "core" glycolipid of Enterobacteriaceae.

Enhanced survival in Pseudomonas aeruginosa septicemia associated with high levels of circulating antibody to Escherichia coli endotoxin core.

“…Antibodies to O-specific side chains in the variable outer region of lipopolysaccharides are species-and typespecific, with their protective activity derived from their ability to promote the phagocytosis and killing of infecting organisms by host phagocytes (3). In contrast, antibodies directed toward the lipid A-containing inner core region are broadly cross-reactive among a wide variety of Gram-negative bacteria (4-7) and are thought to act by neutralizing the biological activities of endotoxin (8)(9)(10)(11).…”

Escherichia coli antibodies in opsonisation and protection against infection

“…However, in other studies (Wolberg and DeWitt, 1969) antisomatic 0 antibodies, raised against the 0-antigenic polysaccharide side chains of the lipopolysaccharide were opsonic and protective for a strain containing K antigen. In addition, several investigators have reported that antisera raised in horses and rabbits against rough mutants of Enterobacteriaceae (strains with lipopolysaccharides lacking 0-antigenic side chains) and containing antibodies against the core-glycolipid antigen, could passively transfer broad-spectrum protection against parenteral challenge with smooth strains (with 0-antigenic side chains) of Enterobacteriaceae (McCabe, 1972;Ziegler et al, 1973;Young, Stevens and Ingram, 1975). These antibodies appear to act primarily as anti-endotoxic antibodies rather than as opsonins .…”

Escherichia Coli K Antigen in Relation to Serum-Induced Lysis and Phagocytosis

“…LPS contains the 0 antigen linked to lipid A through a common core structure (Luderitz, Staub and Westphal, 1966). Antibodies against antigenic determinants in the core region have been reported to be protective against the sequelae of bacteremia due to gram-negative bacilli (McCabe, 1972;Young, Stevens and Ingram, 1975). These antibodies seem to act primarily as antitoxins rather than as opsonins (Braude, Douglas and Davis, 1973).…”