Functional Restoration of ACD‐Blood by Pyridoxal 5′‐Phosphate

Abstract

Successful application of pyridoxal 5'-phosphate (PLP) to restore the oxygen transport function of ACD-stored blood is described. PLP is readily incorporated into ACD-erythrocytes by both carrier-mediated transport (in which ATP may participate) and passive diffusion. Plasma proteins (up to 2.5%) and inorganic phosphate (up to 40 mM) do not affect the incorporation of PLP, though more than 25 mM inorganic phosphate is necessary for the maintenance of ATP levels. Increasing the PLP concentration and/or decreasing the packed cell volume in the medium, increases the incorporation of PLP. Incubation of erythrocytes with PLP at pH 7.0 is most suitable for incorporation of PLP and the maintenance of ATP levels. PLP incorporated into erythrocytes restores the oxygen transport function of the ACD-erythrocytes, though decreased haem--haem interaction is observed. A procedure for the clinical application of PLP-loaded erythrocytes is suggested.

Electrostatic Repulsion among Red Blood Cells: Observed by Macromolecule-Induced Cell Aggregation and Flow Behavior of the Cells in Microtubes

“…2),7), 8) As generally recognized, the RBC aggregation is accelerated by the increase of high molecular weight substances in plasma, such as fibrinogen, immunoglobulins, pathological proteins, infused dextrans and so on. 7),9), 10) The aggregation is also affected by morphological and biochemical characteristics of RBCs. It is well known that sialic acid residue in RBC membrane glycoproteins (i.e., the surface negative charge of the cells) provides an electrostatic repulsive force among RBCs, 11) and that the removal of sialic acid from the RBC surface accelerates RBC aggregation by macromolecules.…”

A lattice Boltzmann method for a two-phase flow containing solid bodies with viscoelastic membranes

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