“…However, we detected little enrichment of our class switch recombination signature in non-cycling DZ GC B cells (Figure 3L), and although there was an enrichment of class switch recombination genes in cycling B cell populations, this likely reflects their involvement in cell cycle-linked DNA recombination and repair. Our analysis also highlights many other genes previously linked with class switch recombination (Figure 3M), including those capable of binding to switch region sequences within the IgH locus ( NME2, NCL, DDX21 ), interacting with the class switch recombination machinery ( NPM1, SERBP1 ) or regulating AICDA /AICDA transcript/protein stability ( mir155HG, HSP90AB1 ) (Borggrefe et al, 1998, Hanakahi et al, 1997, Mondal et al, 2016, Orthwein et al, 2010, Shinozaki et al, 2006, McRae et al, 2017, Zheng et al, 2019). Notably, the microRNA gene miR155HG , formerly called BIC (B-cell Integration Cluster), was up-regulated in preGC B cells and has been shown to be essential for B cells to form GCs and undergo class switch recombination in mice (Thai et al, 2007, Vigorito et al, 2007).…”