Functional Repurposing Revealed by Comparing S. pombe and S. cerevisiae Genetic Interactions

Frost, Adam; Elgort, Marc G.; Brandman, Onn; Ives, C. W.; Collins, Sean R.; Miller-Vedam, Lakshmi E.; Weibezahn, Jimena; Hein, Marco Y.; Poser, Ina; Mann, Matthias; Hyman, Anthony A.; Weissman, Jonathan S.

doi:10.1016/j.cell.2012.04.028

Cited by 146 publications

(181 citation statements)

References 73 publications

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“…Consistent with their localization, the STRIPAK complex from these two species has been reported to play roles in mitosis (9,12,20). Our failure to detect localization of the yeast Far complex components and even the Far9 truncation mutant without its tail-anchor domain to the spindle pole body is consistent with the hypothesis of Frost et al (9) that the function of the STRIPAK complex may have been "repurposed" and the S. cerevisiae STRIPAK complex has lost its function in mitosis.…”

Section: Discussionsupporting

confidence: 90%

“…Human SLMAP and the S. pombe STRIPAK complex also localize to the centrosome and the spindle pole body, respectively (9,12,20). Consistent with their localization, the STRIPAK complex from these two species has been reported to play roles in mitosis (9,12,20).…”

Section: Discussionmentioning

confidence: 63%

“…This arrangement likely facilitates the regulation of the activity of protein kinases by PP2A (3,8). Both Striatin 3 and STRIP1 localize to the Golgi, and depletion of either results in similar defects suggesting they perform similar functions in regulation of Golgi morphology and mitosis (3,9). The SLMAP gene has several splice variants, encoding tail-anchored membrane proteins that associate with the sarcolemmal membrane in muscle cells, the endoplasmic reticulum and the mitochondrial membrane in nonmuscle cells, and the centrosome and the outer nuclear envelope (9 -13).…”

mentioning

confidence: 99%

“…The SLMAP gene has several splice variants, encoding tail-anchored membrane proteins that associate with the sarcolemmal membrane in muscle cells, the endoplasmic reticulum and the mitochondrial membrane in nonmuscle cells, and the centrosome and the outer nuclear envelope (9 -13). SLMAP is required for myoblast fusion, centrosome function, and structural arrangement of the excitation-contraction coupling apparatus in cardiomyocytes (9,12,14,15). Much remains to be determined about the role of the STRIPAK components, the mechanism of their regulation, and the identity of their substrates.…”

mentioning

confidence: 99%

“…In the ascomycete Sordaria macrospora, the STRIPAK complex is required for sexual development and vegetative hyphal fusion (18,19). In the fission yeast Schizosaccharomyces pombe, components of the STRIPAK complex localize to the mitotic spindle pole body in early mitosis and are required for the establishment of asymmetry of the septation initiation network, a conserved signaling pathway that is required for cytokinesis and mitotic transitions (9,20).…”

mentioning

confidence: 99%

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Tiered Assembly of the Yeast Far3-7-8-9-10-11 Complex at the Endoplasmic Reticulum

Pracheil

Liu

2013

Journal of Biological Chemistry

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Background: The Far3-7-8-9-10-11 complex, part of the yeast striatin-interacting phosphatase and kinase (STRIPAK) complex, mediates target of rapamycin complex 2 (TORC2) signaling. Results: The Far3-7-8-9-10-11 complex follows tiered assembly at the endoplasmic reticulum (ER). Conclusion: ER localization of Far9 is required for optimal function in TORC2 signaling. Significance: Our study provides insights into the organization of the yeast STRIPAK complex.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 63%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Tiered Assembly of the Yeast Far3-7-8-9-10-11 Complex at the Endoplasmic Reticulum

Pracheil

Liu

2013

Journal of Biological Chemistry

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Analysis and Annotation of Whole‐Genome or Whole‐Exome Sequencing–Derived Variants for Clinical Diagnosis

Worthey¹

2013

CP Human Genetics

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Over the last several years, next-generation sequencing (NGS) has transformed genomic research through substantial advances in technology and reduction in the cost of sequencing, and also in the systems required for analysis of these large volumes of data. This technology is now being used as a standard molecular diagnostic test under particular circumstances in some clinical settings. The advances in sequencing have come so rapidly that the major bottleneck in identification of causal variants is no longer the sequencing but rather the analysis and interpretation. Interpretation of genetic findings in a clinical setting is scarcely a new challenge, but the task is increasingly complex in clinical genome-wide sequencing given the dramatic increase in dataset size and complexity. This increase requires the development of novel or repositioned analysis tools, methodologies, and processes. This unit provides an overview of these items. Specific challenges related to implementation in a clinical setting are discussed.

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Analysis and Annotation of Whole‐Genome or Whole‐Exome Sequencing Derived Variants for Clinical Diagnosis

Worthey

2017

CP Human Genetics

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Over the last 10 years, next-generation sequencing (NGS) has transformed genomic research through substantial advances in technology and reduction in the cost of sequencing, and also in the systems required for analysis of these large volumes of data. This technology is now being used as a standard molecular diagnostic test in some clinical settings. The advances in sequencing have come so rapidly that the major bottleneck in identification of causal variants is no longer the sequencing or analysis (given access to appropriate tools), but rather clinical interpretation. Interpretation of genetic findings in a complex and ever changing clinical setting is scarcely a new challenge, but the task is increasingly complex in clinical genome-wide sequencing given the dramatic increase in dataset size and complexity. This increase requires application of appropriate interpretation tools, as well as development and application of appropriate methodologies and standard procedures. This unit provides an overview of these items. Specific challenges related to implementation of genome-wide sequencing in a clinical setting are discussed. © 2017 by John Wiley & Sons, Inc.

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Functional Repurposing Revealed by Comparing S. pombe and S. cerevisiae Genetic Interactions

Cited by 146 publications

References 73 publications

Tiered Assembly of the Yeast Far3-7-8-9-10-11 Complex at the Endoplasmic Reticulum

Tiered Assembly of the Yeast Far3-7-8-9-10-11 Complex at the Endoplasmic Reticulum

Analysis and Annotation of Whole‐Genome or Whole‐Exome Sequencing–Derived Variants for Clinical Diagnosis

Analysis and Annotation of Whole‐Genome or Whole‐Exome Sequencing Derived Variants for Clinical Diagnosis

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