Functional regulatory roles of microRNAs in atherosclerosis

Gao, Ya; Peng, Juan; Zhong, Ren; He, Ni-Ya; Li, Qing; Zhao, Xueshan; Wang, Meimei; Wen, Hongyan; Tang, Zhi‐Han; Jiang, Zhi‐Sheng; Wang, Guixue; Liu, Lu‐Shan

doi:10.1016/j.cca.2016.06.044

Cited by 42 publications

(26 citation statements)

References 80 publications

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“…5, 6 The regulation of microRNAs (miRs) in gene therapy has attracted more and more attention in recent years, and it may provide a new target for SCI treatment. 7 …”

Section: Introductionmentioning

confidence: 99%

Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats

et al. 2017

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Our study aims to explore the effects of lentivirus-mediated microRNA-124 (miR-124) gene-modified bone marrow mesenchymal stem cell (BMSC) transplantation on the repair of spinal cord injury (SCI) in rats. BMSCs were isolated from the bone marrow of rats. The target gene miR-124 was identified using a luciferase-reporter gene assay. Seventy-two rats were selected for construction of the SCI model, and the rats were randomly divided into the blank group, sham group, SCI group, negative control (NC) group, overexpressed miR-124 group and si-PDXK group. The mRNA expression of miR-124 and the mRNA and protein expression of pyridoxal kinase (PDXK) were detected by quantitative real-time polymerase chain reaction and western blotting. The locomotor capacity of the rats was evaluated using the Basso, Beattie and Bresnahan (BBB) scale. Brdu, neuron-specific enolase (NSE), neurofilament (NF) and microtubule-associated protein 2 (MAP2) were detected using immunohistochemistry. The expression levels of thyrotropin-releasing hormone (TRH), prostacyclin (PGI2) and gangliosides (GM) were measured using an enzyme-linked immunosorbent assay. PDXK was identified as the target gene of miR-124. The overexpressed miR-124 group exhibited higher miR-124 expression than the SCI, NC and si-PDXK groups. Compared with the SCI and NC groups, the PDXK expression was downregulated in the overexpressed miR-124 and si-PDXK groups, and the BBB scores were significantly increased 7, 21 and 35 days after transplantation. The double-labeled positive cell densities (Brdu+NSE/NF/MAP2) and the expression levels of TRH, PGI2 and GM in the overexpressed miR-124 group were significantly higher than those in the NC and SCI groups. These results indicated that miR-124 targeted PDXK to accelerate the differentiation of BMSCs into neurocytes and promote SCI repair.

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“…5, 6 The regulation of microRNAs (miRs) in gene therapy has attracted more and more attention in recent years, and it may provide a new target for SCI treatment. 7 …”

Section: Introductionmentioning

confidence: 99%

Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats

et al. 2017

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“…By the experimental model, Colombo PC has proved that venous congestion led to endothelial cell activation and releases of inflammatory mediators particular in atherosclerosis [27-29, 31, 32]. Mounting evidence has proved that microRNAs control the senescence and dysfunction of endothelial cells, proliferation and migration of vascular smooth muscle cells, and macrophage-drive cytokine production [33]. Among these endothelial microRNAs, the functional roles of miR-126 and miR-143/145 have been well documented [27].…”

Section: Discussionmentioning

confidence: 99%

Expression Changes of Long Noncoding RNA in the Process of Endothelial Cell Activation

Zhou

Ding

Chen

et al. 2017

Cell Physiol Biochem

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Background: Endothelial cells have been shown to be in response to a variety of local and systemic stimuli, and are able to transition between quiescent and activated states. Endothelial cell activation is critical for the pathogenesis of various cardiovascular diseases. However, the expression changes of long non-coding RNAs (lncRNAs) are still unknown in the process of endothelial cell activation. Thus, this study was aimed to investigate expression changes of lncRNA before and after endothelial cell activation. Materials and Methods: In an experimental model of peripheral venous congestion, endothelial cells were activated and analyzed with Affymetrix HG-U133 plus2.0 microarray. We analyzed these microarray data and reannotated the microarray probes for lncRNA. Results: According to the definition of absolute fold change>2 and p value <0.05, 27 differentially expressed lncRNAs were identified and only 1 lncRNA transcript, ENST00000509256 was down-regualted. Co-expression network of lncRNA and mRNA were constructed to predict function of the dysregulated lncRNA. Gene set enrichment analyses suggested that these ENST00000509256 was associated with many important functions, such as cell-cell signaling and regulation of cell differentiation. Conclusion: Many lncRNAs are dysregulated upon endothelial cell activation and further experiments are needed to identify the potential biological functions of these lncRNAs.

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“…MiRNAs, or microRNAs, are endogenous, non‐coding RNAs that are involved in the regulation of post‐transcriptional gene expression in vivo, thereby regulating a variety of cellular characteristics, such as cell proliferation, cell migration, cell differentiation, and apoptosis (Barwari et al, ; Gao et al, ; Orlicka‐Plocka et al, ). Abnormal miRNA expression has been shown to be associated with cardiovascular disease (Fan et al, ).…”

Section: Introductionmentioning

confidence: 99%

The role of microRNAs in the involvement of vascular smooth muscle cells in the development of atherosclerosis

Tang

2019

Cell Biology International

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MicroRNAs (miRNAs) are a class of nonprotein‐encoding RNAs of ~22 nucleotides in length that bind to or complement each other with a target gene messenger RNA (mRNA) to promote mRNA degradation or inhibit translation of the target mRNA. The protein required [such as Toll‐like receptor (TLR) proteins] is controlled at an optimal level. By affecting protein translation, miRNAs have become powerful regulators of biological processes, including development, differentiation, cell proliferation, and apoptosis. MiRNAs are involved in the regulation of proliferation, migration, and apoptosis of vascular smooth muscle cells (VSMCs), thereby affecting the formation of atherosclerosis (AS). In recent years, the role and mechanism of miRNAs involved in AS development in VSMCs have been studied extensively. In the current study, the results and progress in miRNA research are reviewed.

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Functional regulatory roles of microRNAs in atherosclerosis

Cited by 42 publications

References 80 publications

Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats

Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats

Expression Changes of Long Noncoding RNA in the Process of Endothelial Cell Activation

The role of microRNAs in the involvement of vascular smooth muscle cells in the development of atherosclerosis

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