Functional Regulation of Pre-B-cell Leukemia Homeobox Interacting Protein 1 (PBXIP1/HPIP) in Erythroid Differentiation

Manavathi, Bramanandam; Lo, Dennis; Bugide, Suresh; Dey, Oindrilla; Imren, Suzan; Weiss, Mitchell J.; Humphries, R. Keith

doi:10.1074/jbc.m111.289843

Cited by 37 publications

(26 citation statements)

References 46 publications

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“…These results indicate that HPIP expression plays an important role in EOC development and is significantly associated with an independent poor prognostic factor. Our results are consistent with the previous findings on the roles of HPIP in tumor progression in various cancers 17–19. All these findings suggest an important tumor biological role of HPIP in tumorigenesis and malignant transformation.…”

Section: Discussionsupporting

confidence: 93%

Expression of HPIP in epithelial ovarian carcinoma: a clinicopathological study

Wang

Meng

Liu

et al. 2016

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ObjectivesHematopoietic pre-B-cell leukemia transcription factor (PBX)-interacting protein (HPIP) plays an important role in cancer invasion and metastasis. The aim of this study is to investigate the expression of HPIP in epithelial ovarian cancer (EOC).Patients and methodsImmunohistochemical method was performed using 42 normal ovarian specimens and 145 specimens with EOC. The correlations of HPIP expression with the clinicopathological factors and prognosis of EOC patients were evaluated. Statistical analyses were performed using the chi-square test, multivariate Cox proportional hazard, and Kaplan–Meier method.ResultsHPIP expression in EOC was higher than that in normal tissues (P<0.001). HPIP expression was significantly associated with histological grade, International Federation of Gynecology and Obstetrics stage, and lymphatic metastasis of EOC (P<0.05). Patients with high HPIP expression had poorer overall survival and disease-free survival (P<0.001) compared with patients with low HPIP expression. Multivariate Cox analysis demonstrated that HPIP was an independent factor for overall survival and disease-free survival (P<0.05).ConclusionHPIP may be a valuable biomarker for predicting the prognosis of EOC patients and may serve as a potential target for cancer therapy.

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Section: Discussionsupporting

confidence: 93%

Expression of HPIP in epithelial ovarian carcinoma: a clinicopathological study

Wang

Meng

Liu

et al. 2016

OTT

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“…To determine if O-GlcNAcylation is involved in erythroid differentiation, we treated GATA-1-ER cells with E2 to trigger erythroid differentiation. Expression of GATA-1-ER increased as previously reported (Lamonica et al 2011;Manavathi et al 2012); Gata2 transcription was repressed by GATA-1, resulting in the decrease of GATA-2 protein level (Bresnick et al 2010). Expression of Friend of GATA-1 (FOG-1) also increased as previously reported (Welch et al 2004) (Figure 1a).…”

Section: O-glcnac Levels Decrease During Erythropoiesissupporting

confidence: 85%

O-GlcNAc Homeostasis Controls Cell Fate Decisions During Hematopoiesis

Zhang

Parker

Graw

et al. 2018

Preprint

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The addition of O-GlcNAc (a single β-D-N-acetylglucosamine sugar at serine and threonine residues) by O-GlcNAc transferase (OGT) and removal by O-GlcNAcase (OGA) maintains homeostatic levels of O-GlcNAc. We investigated the role of O-GlcNAc homeostasis in hematopoiesis utilizing G1E-ER4 cells carrying a GATA-1 transcription factor fused to the estrogen receptor (GATA-1ER) that undergo erythropoiesis following the addition of β-estradiol (E2) and myeloid leukemia cells that differentiate into neutrophils in the presence of all-trans retinoic acid. During G1E-ER4 differentiation, a decrease in overall O-GlcNAc levels and an increase in GATA-1 interactions with OGT and OGA were observed. Transcriptome analysis on G1E-ER4 cells differentiated in the presence of Thiamet-G (TMG), an OGA inhibitor, identified expression changes in 433 GATA-1 target genes. Chromatin immunoprecipitation demonstrated that the occupancy of GATA-1, OGT, and OGA at Laptm5 gene GATA site was decreased with TMG. Myeloid leukemia cells showed a decline in O-GlcNAc levels during differentiation and TMG reduced the expression of genes involved in differentiation. Sustained treatment with TMG in G1E-ER4 cells prior to differentiation caused a reduction of hemoglobin positive cells during differentiation. Our results show that alterations in O-GlcNAc homeostasis disrupt transcriptional programs causing differentiation errors suggesting a vital role of O-GlcNAcylation in control of cell fate.

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“…As expected, based on cohesin interactions with Mediator and with CTCF (Kagey et al, 2010; Xiao et al, 2011), MED1 and cohesin loading factor NIPBL can immunoprecipitate CTCF and cohesin component SMC1 (Figure 3A). Similarly, antibodies to GATA1 immunoprecipitate CTCF and MED1 (Stumpf et al, 2006; Manavathi et al, 2012) and SMC1, possibly indirectly (Xiao et al, 2011). Consistent with GATA1 principally functioning as part of the LDB1 complex (Li et al, 2013), LDB1 antibodies also immunoprecipitate CTCF and SMC1, although not MED1.…”

Section: Resultsmentioning

confidence: 99%

The LDB1 Complex Co-opts CTCF for Erythroid Lineage-Specific Long-Range Enhancer Interactions

et al. 2017

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SUMMARY Lineage-specific transcription factors are critical for long-range enhancer interactions but direct or indirect contributions of architectural proteins such as CTCF to enhancer function remain less clear. The LDB1 complex mediates enhancer-gene interactions at the β-globin locus through LDB1 self-interaction. We find that a LDB1-bound enhancer upstream of carbonic anhydrase 2 (Car2) activates its expression by interacting directly with CTCF at the gene promoter. Both LDB1 and CTCF are required for enhancer-Car2 looping and the domain of LDB1 contacted by CTCF is necessary to rescue Car2 transcription in LDB1 deficient cells. Genome wide studies and CRISPR/Cas9 genome editing indicate that LDB1-CTCF enhancer looping underlies activation of a substantial fraction of erythroid genes. Our results provide a mechanism by which long-range interactions of architectural protein CTCF can be tailored to achieve a tissue-restricted pattern of chromatin loops and gene expression.

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Functional Regulation of Pre-B-cell Leukemia Homeobox Interacting Protein 1 (PBXIP1/HPIP) in Erythroid Differentiation

Cited by 37 publications

References 46 publications

Expression of HPIP in epithelial ovarian carcinoma: a clinicopathological study

Expression of HPIP in epithelial ovarian carcinoma: a clinicopathological study

O-GlcNAc Homeostasis Controls Cell Fate Decisions During Hematopoiesis

The LDB1 Complex Co-opts CTCF for Erythroid Lineage-Specific Long-Range Enhancer Interactions

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