Functional reconstruction of corneal endothelium using nanotopography for tissue-engineering applications

Teo, Benjamin Kim Kiat; Goh, Julian Park Nam; Ng, Zheng Jie; Koo, Stephanie; Yim, Evelyn K.F.

doi:10.1016/j.actbio.2012.04.020

Cited by 39 publications

(37 citation statements)

References 48 publications

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“…Identifying the growth conditions that support the growth of cells with a transcriptome profile similar to evHCEnC will provide researchers with a more accurate model of in vivo HCEnC and a potential source of endothelial cells for management of endothelial dysfunction. Optimization of HCEnC culturing techniques should take into consideration several anatomic and physiologic features of in vivo HCEnC (60), such as: 1) adherence to a complex milieu of extracellular matrix proteins (13,18,22,38,40,51,57); 2) contact with physiological proteins and other factors present in aqueous humor (25,33,36,42,53); 3) exposure to appropriate biomechanical forces (35,40,57); and 4) maintenance of a confluent semipermeable layer of cells with strong apicobasal polarity. Replication of each of these features in a single culturing method would be challenging, but would represent a significant advance in the development of cultured HCEnC that closely resemble in vivo HCEnC.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement Therapy

Frausto

Aldave

2016

Cell Transplant

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The corneal endothelium plays a primary role in maintaining corneal homeostasis and clarity, and must be surgically replaced with allogenic donor corneal endothelium in the event of visually significant dysfunction. However, a worldwide shortage of donor corneal tissue has led to a search for alternative sources of transplantable tissue. Cultured human corneal endothelial cells (HCEnC) have been shown to restore corneal clarity in experimental models of corneal endothelial dysfunction in animal models, but characterization of cultured HCEnC remains incomplete. To this end, we utilized next-generation RNA sequencing technology to compare the transcriptomic profile of ex vivo human corneal endothelial cells (evHCEnC) with that of primary HCEnC (pHCEnC) and HCEnC lines, and to determine the utility of cultured and immortalized corneal endothelial cells as models of in vivo corneal endothelium. Multidimensional analyses of the transcriptome datasets demonstrated that primary HCEnC have a closer relationship to evHCEnC than do immortalized HCEnC. Subsequent analyses showed that the majority of the genes specifically expressed in HCEnC (not expressed in ex vivo corneal epithelium or fibroblasts) demonstrated a marked variability of expression in cultured cells compared with evHCEnC. In addition, genes associated with either corneal endothelial cell function or corneal endothelial dystrophies were investigated. Significant differences in gene expression and protein levels were observed in the cultured cells compared with evHCEnC for each of the genes tested except for AGBL1 and LOXHD1, which were not detected by RNA-seq or qPCR. Our transcriptomic analysis suggests that at a molecular level pHCEnC most closely resemble evHCEnC and thus represent the most viable cell culture based therapeutic option for managing corneal endothelial cell dysfunction. Our findings also suggest that investigators should perform an assessment of the entire transcriptome of cultured HCEnC prior to determination of their potential clinical utility for the management of corneal endothelial cell failure.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement Therapy

Frausto

Aldave

2016

Cell Transplant

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“…They observed that the nano pillars provide better support than the micro wells/pillars and enhance corneal endothelial regeneration by increasing the Na + /K + ATPase activity and upregulating messenger ribonucleic acid. 100 In another study, superparamagnetic nanoparticles with tropomyosin-related kinase B agonist antibodies have been used to activate the tropomyosin-related kinase B-dependent signaling pathway which, in turn, promotes neurite growth. These findings hold potential for using functionalized superparamagnetic nanoparticles to treat injury in the CNS.…”

Section: Ocular Regenerationmentioning

confidence: 99%

Regenerative nanomedicine: current perspectives and future directions

Chaudhury¹,

Kumar²,

Kandasamy³

et al. 2014

IJN

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Nanotechnology has considerably accelerated the growth of regenerative medicine in recent years. Application of nanotechnology in regenerative medicine has revolutionized the designing of grafts and scaffolds which has resulted in new grafts/scaffold systems having significantly enhanced cellular and tissue regenerative properties. Since the cell–cell and cell-matrix interaction in biological systems takes place at the nanoscale level, the application of nanotechnology gives an edge in modifying the cellular function and/or matrix function in a more desired way to mimic the native tissue/organ. In this review, we focus on the nanotechnology-based recent advances and trends in regenerative medicine and discussed under individual organ systems including bone, cartilage, nerve, skin, teeth, myocardium, liver and eye. Recent studies that are related to the design of various types of nanostructured scaffolds and incorporation of nanomaterials into the matrices are reported. We have also documented reports where these materials and matrices have been compared for their better biocompatibility and efficacy in supporting the damaged tissue. In addition to the recent developments, future directions and possible challenges in translating the findings from bench to bedside are outlined.

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“…Fig. 5(a) shows an optical image of fixed B4G12 cells with an average area of about 600 mm 2 [21], while Fig. 5(b) shows a phase contrast image of a pair of adjacent B4G12 cells which appears flat and tightly bound to the cover slip.…”

Section: Resultsmentioning

confidence: 98%

Imaging of soft material with carbon nanotube tip using near-field scanning microwave microscopy

Wu¹,

Sun²,

Feng³

et al. 2015

Ultramicroscopy

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Functional reconstruction of corneal endothelium using nanotopography for tissue-engineering applications

Cited by 39 publications

References 48 publications

Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement Therapy

Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement Therapy

Regenerative nanomedicine: current perspectives and future directions

Imaging of soft material with carbon nanotube tip using near-field scanning microwave microscopy

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