Functional protection of pentoxifylline against spinal cord ischemia/reperfusion injury in rabbits: necrosis and apoptosis effects

Zhu, Danjie; Xia, Bing; Bi, Qing; Zhang, Shuijun; Qiu, Bin-Song; Chen, Zhao

doi:10.1097/00029330-200812010-00016

Cited by 6 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In accordance with our results, others have reported that PTX may induce protection against ischemia injury in the spinal cord in rabbits by preventing both necrosis and apoptosis. The study also reported a significant decrease in serum TNF-α and spinal cord caspase-3 activity in the presence of PTX [52]. Thus, confirming a protective mechanism of the drug.…”

Section: Discussionsupporting

confidence: 64%

Pentoxifylline suppressed LPS-induced inflammatory and apoptotic signaling in neuronal cells

Muchhala¹,

Benzeroual

2012

ABB

View full text Add to dashboard Cite

Several studies have reported a relation between increased pro-inflammatory mediators such as TNF-α and apoptosis in neurodegenerative diseases such as Alzheimer’s disease (AD). It is known that lipopoly-saccharide (LPS) treatment induces neuroinflammation and memory deterioration, and it has been reported that LPS induces apoptosis mostly through the production of TNF-α. Pentoxifylline (PTX), is a vascular protective agent and a potent TNF-alpha inhibitor. However, the molecular neuroprotective mechanisms of PTX against LPS-induced neurotoxicity have not been well studied. In this study, we investigated the direct protective effect of PTX against LPS-induced toxicity in Rat pheochromocytoma (PC12) cell line. The results showed that a pretreatment with PTX prior exposure to LPS signifycantly decreased LPS-induced cell death. Mechanisms study showed that PTX has the potential to inhibit pro-inflammatory and pro-apoptotic pathways via the suppression of TNF-α and a caspase-dependent pathway in neuronal PC12 cells. This is the first study to report the anti-inflammatory and anti-apoptotic effects of PTX via inhibition of TNF-α and a caspase-dependent pathway in neuronal PC12 cells. Altogether, these observations indicate that PTX is capable of promoting neuroprotective effects, meanwhile also present some insights into the potential signaling pathways that are involved. Thus, these findings support the potential for PTX to be investigated as a potential agent for the treatment of neurodegenerative diseases such as AD

show abstract

Section: Discussionsupporting

confidence: 64%

Pentoxifylline suppressed LPS-induced inflammatory and apoptotic signaling in neuronal cells

Muchhala¹,

Benzeroual

2012

ABB

View full text Add to dashboard Cite

show abstract

“…Since TNF- α is an important contributor to spinal cord IRI which might induce necrosis and apoptosis of cells, its inhibitor might exhibit a protective role in spinal cord injury following ischemia [32]. In a rabbit model of spinal cord IRI (45 minutes cross-clamping of the infrarenal aorta), a significant decrease in both necrotic and apoptotic neurons was observed in the Pentoxifylline-treated groups compared with the IRI group ( P < 0.05) [32]. …”

Section: Treatments For Inflammation In Spinal Cord Irimentioning

confidence: 99%

Development and Treatments of Inflammatory Cells and Cytokines in Spinal Cord Ischemia-Reperfusion Injury

Zhu

Fujino

et al. 2013

Mediators of Inflammation

View full text Add to dashboard Cite

During aortic surgery, interruption of spinal cord blood flow might cause spinal cord ischemia-reperfusion injury (IRI). The incidence of spinal cord IRI after aortic surgery is up to 28%, and patients with spinal cord IRI might suffer from postoperative paraplegia or paraparesis. Spinal cord IRI includes two phases. The immediate spinal cord injury is related to acute ischemia. And the delayed spinal cord injury involves both ischemic cellular death and reperfusion injury. Inflammation is a subsequent event of spinal cord ischemia and possibly a major contributor to spinal cord IRI. However, the development of inflammatory mediators is incompletely demonstrated. And treatments available for inflammation in spinal cord IRI are insufficient. Improved understanding about spinal cord IRI and the development of inflammatory cells and cytokines in this process will provide novel therapeutic strategies for spinal cord IRI. Inflammatory cytokines (e.g., TNF-α and IL-1) may play an important role in spinal cord IRI. For treatment of several intractable autoimmune diseases (e.g., rheumatoid arthritis), where inflammatory cytokines are involved in disease progression, anti-inflammatory cytokine antagonist is now available. Hence, there is great potential of anti-inflammatory cytokine antagonist for therapeutic use of spinal cord IRI. We here review the mediators and several possibilities of treatment in spinal cord IRI.

show abstract

“…An earlier study [ 25 ] indicated that TNF-alpha and glutamate could act synergistically to induce neuronal cell death. Recently, many anti-inflammatory properties of PTX have been described, including the decrease of inflammatory mediator production and the prevention of neuronal apoptosis, and decreased caspase-3 activity [ 26 ]. The study data indicate that the number of apoptotic bodies significantly decreased with PTX treatment.…”

Section: Discussionmentioning

confidence: 99%

Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Movassaghi

Koohpar

Hashemi

et al. 2019

GMJ

View full text Add to dashboard Cite

Background: 3,4-Methylenedioxymethamphetamine is psychoactive and hallucinogenic and has been shown to produce neurotoxicity both in animals and in humans. Recently, vasodilator drugs such as pentoxifylline (PTX) have been introduced as an alternative with neuroprotective effects. There is no study about the protective effect of PTX on hippocampal apoptosis due to high-dose administration of 3,4-Methylenedioxymethamphetamine (MDMA), so in this study, the protective effect of PTX on the hippocampus of male Wistar rats following high-dose of the drug has been investigated. Materials and Methods: Twenty-four male Wistar rats weighing 250-300 g were randomly divided into four groups: control, sham (MDMA injection), experimental (MDMA+PTX injection), and vehicle (MDMA+saline) groups. Two weeks later, the brains were removed and prepared for TUNEL and western blot techniques. Concomitantly the hippocampus was removed to study the change in Bcl-2 and BAX mRNA expression with quantitative real-time polymerase chain reaction. Results: Data showed that the number of apoptotic bodies significantly decreased in the experimental group compared to the other groups, except for in control. Also, further investigation revealed that BAX reduced considerably, while Bcl-2 mRNA expression increased dramatically after PTX treatment. Conclusions: Our results suggest that PTX may be a neuroprotective agent, and its neuroprotective potential may contribute to reducing the severity of lesions in the hippocampus following a high dose administration of MDMA. [GMJ.2019;8:e963]

show abstract

Functional protection of pentoxifylline against spinal cord ischemia/reperfusion injury in rabbits: necrosis and apoptosis effects

Cited by 6 publications

References 25 publications

Pentoxifylline suppressed LPS-induced inflammatory and apoptotic signaling in neuronal cells

Pentoxifylline suppressed LPS-induced inflammatory and apoptotic signaling in neuronal cells

Development and Treatments of Inflammatory Cells and Cytokines in Spinal Cord Ischemia-Reperfusion Injury

Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Contact Info

Product

Resources

About