Functional properties and expression quantitative trait loci for phosphate transporter GmPT1 in soybean

Abstract

Phosphate (Pi) remobilization within a plant is critical for plant survival under Pi-limiting conditions. In this paper, a soybean Pi transporter gene, GmPT1, was characterized. A marked induction of GmPT1 transcript was observed in young leaves, mature leaves and lateral roots during longterm Pi starvation. Transgenic tobacco plants containing the GmPT1 gene were obtained using an Agrobacteriummediated transformation system. Compared with wild-type plants, transgenic plants showed significant increases in phosphorus-use efficiency (PUE), photosystem II (PSII) function, total dry weight and seed weight under Pi-deficient conditions. GmPT1 expression levels and PUE were determined in a soybean recombinant inbred line population during a pot experiment that was conducted to measure chlorophyll fluorescence parameters, photosynthetic rate (PN) and seed yield. Correlation analysis revealed that GmPT1 expression levels had significantly positive correlations with seed yield, PUE, PN and the quantum yield of PSII primary photochemistry (ΦPSII). Expression quantitative trait loci (eQTL) mapping for GmPT1 revealed two eQTLs, one of which coincided with both the physical location of GmPT1 and a QTL associated with seed yield. These results suggest that GmPT1 plays a role in Pi remobilization, and it may be possible to improve soybean seed yields under Pi-limiting conditions by modulating GmPT1 expression levels.

Genome-wide Analysis of Phosphoenolpyruvate Carboxylase Gene Family and Their Response to Abiotic Stresses in Soybean

“…The chamber had a relative humidity of 70%. The seedlings were then transferred to plastic boxes (1 L) filled with half-strength Hoagland nutrient solution for hydroponic culture64 and incubated in the same growth chamber. The nutrient solution was changed every 3 days.…”

Genetic architecture of biofortification traits in soybean (Glycine max L. Merr.) revealed through association analysis and linkage mapping

“…The first member of the ZIP family of metal transporters identified in soybean, GmZIP1, which is localized in the peribacteroid, is highly selective for Zn, as determined by the functional complementation of zrt1zrt2 in yeast (Moreau et al 2002). In addition, Song et al (2014) introduced GmPT1 into tobacco and discovered a significant increase in shoot P content of transgenic plants compared with wild-type plants. GmACP1 overexpression in soybean roots could lead to transgenic hairy roots secrete more acid phosphatase to catalyze the hydrolysis of q-nitrophenyl phosphate and release inorganic phosphate for soybean absorption (Zhang et al 2014a).…”

Arabidopsis RING E3 ubiquitin ligase AtATL80 is negatively involved in phosphate mobilization and cold stress response in sufficient phosphate growth conditions

“…1DeE). These AtATL80 expression profiles are reminiscent of those of Pi remobilization transporter genes, such as PHT1;5, PHT2;1, and GmPT1 [9,21,26]. Overexpression of AtATL80 caused P accumulation in shoot tissues, lower biomass, and reduced PUE under high Pi (1 mM) conditions compared to wild-type plants (Fig.…”

“…Total P concentration was quantified using a modified version of the U.S. Environmental Protection Agency method 365.2, as recorded by Song et al [21]. Total P content was measured using a spectrophotometer (model DU800; Beckman Coulter, Brea, CA, USA) at 650 nm, and expressed as total P/mg tissue dry weight.…”

“…These results indicate that loss and overexpression of AtATL80 resulted in the decrease and increase, respectively, of P content in shoot tissues under high Pi conditions. P utilization efficiency (PUE) is defined as the amount of biomass produced per unit of total P accumulated in the shoots [21]. When compared with the PUE (2.9 ± 0.1 Â 10 À2 g DW/mg P) of the wild-type plants, that of the atatl80 mutant increased by approximately 17% (3.4 ± 0.2 Â 10 À2 g DW/mg P), whereas the PUE of 35S:AtATL80-sGFP transgenic plants decreased by approximately 35% (1.9 ± 0.1 Â 10 À2 g DW/mg P) to 28% (2.1 ± 0.1 Â 10 À2 g DW/mg P) (Fig.…”