Functional promoters created by the insertion of transposable element IS1

Prentki, Pierre; Teter, Bruce; Chandler, Michaël; Galas, David J.

doi:10.1016/0022-2836(86)90134-8

Cited by 78 publications

(53 citation statements)

References 41 publications

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“…A few notably proliferous transposases have been copied into multiple locations, particularly in fer1 (e.g., seven identical copies of one novel transposase). As sources of strain-level diversity, together with the effects IS elements can elicit on gene expression and genome topography (15)(16)(17)(18)(19)(20), heterogeneous IS dynamics may alter the adaptive fitness of population members population over short time scales.…”

Section: Resultsmentioning

confidence: 99%

Genome dynamics in a natural archaeal population

Allen¹,

Tyson²,

Whitaker³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

118

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Evolutionary processes that give rise to, and limit, diversification within strain populations can be deduced from the form and distribution of genomic heterogeneity. The extent of genomic change that distinguishes the acidophilic archaeon Ferroplasma acidarmanus fer1 from an environmental population of the same species from the same site, fer1(env), was determined by comparing the 1.94-megabase (Mb) genome sequence of the isolate with that reconstructed from 8 Mb of environmental sequence data. The fer1(env) composite sequence sampled Ϸ92% of the isolate genome. Environmental sequence data were also analyzed to reveal genomic heterogeneity within the coexisting, coevolving fer1(env) population. Analyses revealed that transposase movement and the insertion and loss of blocks of novel genes of probable phage origin occur rapidly enough to give rise to heterogeneity in gene content within the local population. Because the environmental DNA was derived from many closely related individuals, it was possible to quantify gene sequence variability within the population. All but a few gene variants show evidence of strong purifying selection. Based on the small number of distinct sequence types and their distribution, we infer that the population is undergoing frequent genetic recombination, resulting in a mosaic genome pool that is shaped by selection. The larger genetic potential of the population relative to individuals within it and the combinatorial process that results in many closely related genome types may provide the basis for adaptation to environmental fluctuations.

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Section: Resultsmentioning

confidence: 99%

Genome dynamics in a natural archaeal population

Allen¹,

Tyson²,

Whitaker³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

118

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“…ISAba2, a novel insertion sequence, and IS18 provided hybrid promoters enhancing bla OXA-58 expression. Such hybrid promoters generated upon transposition have been already observed, for instance, with IS1 and IS2 (14,23) and also with IS18. Indeed, similar to our observations, IS18 identified previously also from an A. baumannii isolate activated a silent aminoglycoside resistance gene by providing a Ϫ35 promoter sequence of an hybrid promoter (24).…”

Section: Discussionmentioning

confidence: 67%

Acinetobacter baumannii

2015

Reviews in Medical Microbiology

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Genetic structures surrounding the carbapenem-hydrolyzing oxacillinase gene bla OXA-58 were characterized in a series of OXA-58-positive Acinetobacter baumannii strains isolated from different countries. We showed that in most of the cases, acquisitions of the bla OXA-58 -containing overall structure, including insertion sequence elements, may be likely the results of recombination events. In type strain A. baumannii MAD, the genetic structure surrounding the bla OXA-58 gene was bracketed by two 27-bp repeated sequences. The isolation of a clonally related OXA-58-negative A. baumannii isolate that possessed the same plasmid backbone as A. baumannii MAD but lacked this genetic structure indicated that the mechanism of acquisition could be reversible. Parts of the structure identified in A. baumannii MAD were conserved in other bla OXA-58 -positive isolates from various European countries. Primer extension experiments showed that bla OXA-58 expression was related to promoter sequences brought by different insertion sequence elements, such as an ISAba3-like element, ISAba1, ISAba2, and IS18. This work identified novel structures at the origin of acquisition and expression of a carbapenem-hydrolyzing ␤-lactamase identified in non-clonally related A. baumannii isolates.

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“…Insertion of IS1 into the promoter P3 of blaT-1 in plasmid pBR322 has been reported (Prentki et al, 1986). The insertion was at the same position as that of the ISIlike element upstream in blaT-6 and resulted in the formation of an active hybrid promoter consisting of the -10 region from P3 and a -35 TTGGCA region provided by the end of ISI.…”

Section: Discussionmentioning

confidence: 98%

“…3). Transposition of ISI results in the duplication of 9 bp of the target (Prentki et al, 1986). Since a 9 bp sequence is repeated directly at the ends of the ISI-like element, it is likely that the presence of the 116 bp DNA fragment is the result of a transposition event.…”

Section: Discussionmentioning

confidence: 99%

“…An explanation for this difference could be that the -35 region of the 'ISI-hybrid promoter' has a poorer match to the canonical -35 consensus than that in P6. Alternatively, features of the regions upstream from the -35 sequences in ISI and in the ISI-like element might be important for promoter recognition by the RNA polymerase (Moran et al, 1982;Prentki et al, 1986). The ISI-like element was detected by PCR, using two oligonucleotides specific for the ends of the insert (Fig.…”

Section: Discussionmentioning

confidence: 99%

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An IS1-like element is responsible for high-level synthesis of extended-spectrum -lactamase TEM-6 in Enterobacteriaceae

Goussard

Sougakoff

Mabilat

et al. 1991

Journal of General Microbiology

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Resistance of Escherichiu coli strain HB251 to the newer p-lactam antibiotics, in particular ceftazidime and aztreonam, results from production of the extended-spectrum p-lactamase TEM-6. The corresponding structural gene, bfuT-6, and its promoter region were amplified by the polymerase chain reaction. Analysis of the sequence of the amplification product showed that bluT-6 differed by two nucleotide substitutions from bluT-1, the gene encoding TEM-1 penicillinase in plasmid pBR322. The mutations led to the substitution of a lysine for a glutamic acid at position 102 and of a histidine for an arginine at position 162 of the unprocessed TEM-1 protein. The presence of a 116 bp DNA insert upstream from bluT-6 resulted in the creation of hybrid promoter P6 in which the -10 region was that of TEM-1 promoter P3 whereas the -35 canonical sequence TTGACA was provided by the right end of the insert. P6 was found to be 10 times more active than P3 and to confer higher levels of antibiotic resistance upon the host. Analysis of the sequence of the insert indicated that the 116 bp fragment is related to insertion sequence IS1 but differs from it by three internal deletions that removed regions encoding the transposase. The distribution of the ISl-like element in clinical isolates of Enterobucferiaceae was studied by the polymerase chain reaction and by DNA-DNA hybridization. The element appeared to be widespread and was detected in strains producing TEM-6 or other TEM variants.

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Functional promoters created by the insertion of transposable element IS1

Cited by 78 publications

References 41 publications

Genome dynamics in a natural archaeal population

Genome dynamics in a natural archaeal population

Acinetobacter baumannii

An IS1-like element is responsible for high-level synthesis of extended-spectrum -lactamase TEM-6 in Enterobacteriaceae

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