Functional products fortified with probiotic LAB isolated from Egyptian dairy sources showed hypolipidemic effects in Albino rats

Abstract

Biological food industry has increased economic importance of new Lactic Acid Bacteria (LAB) with functional aspects such as health promoting and enhanced sensorial traits. The objectives of the present study were the isolation and genotypic characterization (16S rRNA) of new LAB isolates from Egyptian Laban Rayeb, Zabady and mothers’ breast milk as potential probiotics. Strains were in vitro and in vivo evaluated for their safety and probiotic health promoting traits in Albino rats then applied into two types of functional dairy products. Three strains Streptococcus thermophilus MH422542, Enterococcus faecium MH422543 and Lactococcus lactis subsp lactis MH422545, were selected from a pool of 82 strains. St. thermophilus showed proteolytic activity and production of Exopolysaccharides (EPS). When evaluated in animal models; the strains showed hypolipidemic effects especially E. faecium that reduced atherogenic indices up to 88%. The strains modulated the gut microbiota by reducing Staphylococcus sp. and coliforms with no adverse effects either on blood parameters, antioxidant enzymes, cancer markers or hepatocytes histological examination. Only E. faecium MH422543 showed an immune response by increased white blood cells (WBC) while lymph and platelets values were not affected. The probiotic strains approved their convenience as starter/ adjunct cultures and enhanced organoleptic properties of the fermented dairy products.

Effect of probiotics on hematological parameters of male and female Wistar rats

“…Since hematocrit has a direct link with RBCs level, probiotic combinations affected both genders differently. These results were in accordance with Negi et al (2022), and Darwish et al (2022) .…”

“…The lowest levels were seen in 0 day in both genders. These results were in good agreement with Ou et al (2022) , and Darwish et al (2022) .…”

“…However, all values were in the normal range, suggesting no kidney, liver, or any other organ damage. These results were according to Liao et al (2022) , and Darwish et al (2022) .…”

“…However, the difference in MCHC in males and females could be because of a change in the activity of either gender ( Rashid et al, 2016 ). These results were according to Darwish et al, 2022 , Sujaya et al, 2022 , and Ou et al (2022) .…”

The role of gut microbiota in intestinal disease: from an oxidative stress perspective

“…Several studies have been conducted to investigate the potential impact of probiotics on the management of gastrointestinal disorders, including IBS, IBD, necrotizing enterocolitis, and diarrhea ( Goldenberg et al, 2017 ; Gracie et al, 2019 ; Mei et al, 2022 ; Nabavi-Rad et al, 2022 ). The utility of probiotics extends beyond their nutritional value, as they play a crucial role in enhancing and fortifying the existing gut microbiota ( Darwish et al, 2022 ). Indeed, it is possible for them to modify the composition of the indigenous gut microbiota, resulting in a subsequent modification of the gut lumen that promotes a more advantageous anti-inflammatory environment ( Suez et al, 2018 ).…”