Functional potato bioactive peptide intensifies Nrf2-dependent antioxidant defense against renal damage in hypertensive rats

Tsai, Bruce Chi‐Kang; Hsieh, Dennis Jine Yuan; Lin, Wei‐Ting; Tamilselvi, Shanmugam; Day, Cecilia Hsuan; Ho, Tsung Jung; Chang, Ruey Lin; Viswanadha, Vijaya Padma; Kuo, Chia‐Hua; Huang, Chih‐Yang

doi:10.1016/j.foodres.2019.108862

Cited by 56 publications

(35 citation statements)

References 41 publications

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“…The main mechanisms involved in the protective effects of these antioxidants include the reduction of oxidative stress and induction of antioxidant defenses. Therefore, our results suggest that the antioxidant peptides identified in our study, as well as other antioxidant peptides reported elsewhere [ 34 , 40 , 41 ], show better antioxidant activity through the reinforcement of antioxidant defense and the reduction of deleterious ROS production. At the same time, malonaldehyde (MDA), which is considered to be an important bio-marker for oxidative stress, was characterized with a certain degree of decline ( Figure 5 C).…”

Section: Resultssupporting

confidence: 80%

Antioxidant Peptides from Sepia esculenta Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in Caenorhabditis elegans

Shen

et al. 2020

Marine Drugs

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The hydrolysate of golden cuttlefish (Sepia esculenta) was prepared by using papain, and then, it was further separated by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The peptide components of the active fraction were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then two novel peptides, SeP2 (DVEDLEAGLAK, 1159.27 Da) and SeP5 (EITSLAPSTM, 1049.22 Da), were obtained and displayed significant alleviation effects on oxidative stress in Caenorhabditis elegans. Studies indicated that S. esculenta antioxidant peptides (SePs) increase superoxide dismutase (SOD) activity but reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levelsin oxidation-damaged nematodes. Using transgenic CF1553 nematodes, the sod-3p::GFP expression in the worms treated with SePs was significantly higher than that of the control nematodes. Real-time PCR also demonstrated that the expression of stress-related genes such as sod-3 is up-regulated by SePs. Furthermore, studies showed that SePs could obviously decrease fat accumulation as well as reduce the elevated ROS and MDA levels in high-fat nematodes. Taken together, these results indicated that SePs are capable of the activation of antioxidant defense and the inhibition of free radicals and lipid peroxidation, play important roles in attenuating oxidative stress and fat accumulation in C. elegans, and might have the potential to be used in nutraceutical and functional foods.

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Section: Resultssupporting

confidence: 80%

Antioxidant Peptides from Sepia esculenta Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in Caenorhabditis elegans

Shen

et al. 2020

Marine Drugs

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“…This explained a decline in ACE inhibitory activity of these hydrolysates after pancreatic digestion. Previous research indicated that peptides exert cellular antioxidant activity through either acting as direct scavengers or activating cellular signaling such as the nuclear factor erythroid 2−related factor 2 pathway, demonstrating that antioxidant activity of peptides depends both on their amino acid compositions and sequences [ 20 , 46 , 65 , 67 , 68 ]. A higher resistance to the gastrointestinal digestion of SPH-T than those of SPH-26L and SPH-P might be responsible for its more retained antioxidant activity after the gastrointestinal digestion ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

“…Later, mitigation of vascular inflammation and restoration of endothelium-dependent vasorelaxation mediated by upregulation of ACE2-Ang (1–7)-MasR axis was found to be the major mechanism [ 18 ]. Studies have also reported the importance of other antihypertensive-related activities (other than ACE inhibition) in blood pressure control such as antioxidant and anti-inflammatory activities [ 19 , 20 , 21 ]. These findings prompted us to use other biomarkers, such as ACE2 upregulation, anti-oxidation and anti-inflammation, in addition to ACE inhibition, to study the antihypertensive activity of a hydrolysate or peptide.…”

Section: Introductionmentioning

confidence: 99%

Spent Hen Protein Hydrolysate with Good Gastrointestinal Stability and Permeability in Caco-2 Cells Shows Antihypertensive Activity in SHR

Fan

Liao

et al. 2020

Foods

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Spent hens are a major byproduct of the egg industry but are rich in muscle proteins that can be enzymatically transformed into bioactive peptides. The present study aimed to develop a spent hen muscle protein hydrolysate (SPH) with antihypertensive activity. Spent hen muscle proteins were hydrolyzed by nine enzymes, either individually or in combination; 18 SPHs were assessed initially for their in vitro angiotensin-converting enzyme (ACE) inhibitory activity, and three SPHs, prepared by Protex 26L (SPH-26L), pepsin (SPH-P), and thermoase (SPH-T), showed promising activity and peptide yield. These three hydrolysates were further assessed for their angiotensin-converting enzyme 2 (ACE2) upregulating, antioxidant, and anti-inflammatory activities; only SPH-T upregulated ACE2 expression, while all three SPHs showed antioxidant and anti-inflammatory activities. During simulated gastrointestinal digestion, ACE2 upregulating, ACE inhibitory and antioxidant activities of SPH-T were not affected, but those of SPH-26L and SPH-P were reduced. ACE inhibitory activity of gastrointestinal-digested SPH-T was not affected after the permeability study in Caco-2 cells, while ACE2 upregulating, antioxidant and anti-inflammatory activities were improved; nine novel peptides with five–eight amino acid residues were identified from the Caco-2 permeate. Among these three hydrolysates, only SPH-T reduced blood pressure significantly when given orally at a daily dose of 1000 mg/kg body weight to spontaneously hypertensive rats. SPH-T can be developed into a promising functional food ingredient against hypertension, contributing to a more sustainable utilization for spent hens while generating extra revenue for the egg industry.

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“…Genetic models of hypertension, such as spontaneously hypertensive rats (SHRs), are reported to have increased ROS production in their vasculature, while supplementation with antioxidant agents, such as α-tocopherol and ascorbic acid, can attenuate oxidative stress, improve vascular function, and prevent the development of hypertension [16][17][18]. Likewise, food-derived antioxidant peptides have shown abilities in ameliorating excessive oxidative stress in various cell and animal models of hypertension [19][20][21][22][23]. Several spent hen-derived peptides, including IWHH, IWH, and IW, have been reported to reduce the basal oxidative stress in ECs [24].…”

Section: Introductionmentioning

confidence: 99%

Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells

Fan

Bhullar

2021

Antioxidants

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Spent hens are egg-laying hens reaching the end of their egg-laying cycles, being a major byproduct of the egg industry. Recent studies have been focusing on finding new value-added uses for spent hens. We have previously identified four bioactive peptides from spent hen muscle proteins, including three angiotensin-converting enzyme (ACE) inhibitory (ACEi) peptides (VRP, LKY, and VRY), and one ACE2 upregulating (ACE2u) peptide (VVHPKESF (V-F)). In the current study, we further assessed their antioxidant and cytoprotective activities in two vascular cell lines—vascular smooth muscle A7r5 cells (VSMCs) and endothelial EA.hy926 cells (ECs)—upon stimulation by tumor necrosis factor alpha (TNFα) and angiotensin (Ang) II, respectively. The results from our study revealed that all four peptides attenuated oxidative stress in both cells. None of the investigated peptides altered the expression of TNFα receptors in ECs; however, VRY and V-F downregulated Ang II type 1 receptor (AT1R), while V-F upregulated the Mas receptor (MasR) in VSMCs. Further, we found that the antioxidant effects of VRP, LKY, and VRY were likely through acting as direct radical scavengers, while that of V-F was at least partially ascribed to increased endogenous antioxidant enzymes (GPx4 and SOD2) in both cells. Besides, as an ACE2u peptide, V-F exerted antioxidant effect in a MasR-dependent manner, indicating a possible involvement of the upregulated ACE2-MasR axis underlying its antioxidant action. The antioxidant activities of VRP, LKY, VRY, and V-F in vascular cells indicated their multifunctional properties, in addition to their ACEi or ACE2u activity, which supports their potential use as functional food ingredients against hypertension.

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Functional potato bioactive peptide intensifies Nrf2-dependent antioxidant defense against renal damage in hypertensive rats

Cited by 56 publications

References 41 publications

Antioxidant Peptides from Sepia esculenta Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in Caenorhabditis elegans

Antioxidant Peptides from Sepia esculenta Hydrolyzate Attenuate Oxidative Stress and Fat Accumulation in Caenorhabditis elegans

Spent Hen Protein Hydrolysate with Good Gastrointestinal Stability and Permeability in Caco-2 Cells Shows Antihypertensive Activity in SHR

Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells

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