Functional polymer affinity matrix for purifying hexahistidine-tagged recombinant protein

Zeng, Qingbing; Xu, Jiacheng; Fu, Ruowen; Ye, Qiaozhen

doi:10.1016/s0021-9673(01)00863-9

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Cited by 6 publications

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“…In IMAC, the combination of protein, metal ion, chelator ligand, and support material is very important. Choosing the support material is important because of protein retention caused by the support material [18][19][20][21] . For this reason, a variety of support materials have been prepared and studied for protein purification in the last decades [22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

Bentonite-acrylamide-histidine-Cu(II) Microcomposite for Cytochrome c Adsorption

Akkaya

2012

Polymers and Polymer Composites

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Bentonite-acrylamide-histidine with an average particle diameter of 200-1000 μm was produced by bulk polymerization of bentonite, acrylamide, bisacrylamide, AIBN and histidine. Bentonite-acrylamide-histidine-Cu(II) microcomposite was prepared by loading copper ions. The microcomposite was characterized by scanning electron microscopy (SEM), specific surface area and elemental analysis. Cytochrome c adsorption studies were performed, for the first time, by using the new metal chelated affinity support (bentonite-acrylamide-histidine-Cu(II)) microcomposite in batchwise experiments.

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