Functional poly(ε-caprolactone)/chitosan dressings with nitric oxide-releasing property improve wound healing

Zhou, Xin; He, Wei; Zhang, Jimin; Li, Xuemei; Wu, Yifan; Wei, Yongzhen; Ji, Shenglu; Kong, Deling; Zhao, Qiang

doi:10.1016/j.actbio.2017.03.011

Cited by 130 publications

(69 citation statements)

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“…We fabricated cardiac patches consisting of a biodegradable polymer ‘PCL’ and an ECM component ‘collagen type 1’ and incorporated SP and IGF-1C peptides to simultaneously enhance endogenous stem cell mobilization and survival, respectively. The processing parameters of PCL fibers have been optimized to fabricate electrospun membranes to facilitate cellularization and remodeling, which has been documented elsewhere [ 33 ]. The morphology of cardiac patches was assessed by SEM, which revealed the presence of uniform, continuous and smooth fibers ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…IGF-1C peptide could support the cardiac cell survival and retention, attenuate fibrosis, and facilitate the recovery of heart function [ 27 ]. IGF-1 could provide an immediate strong pro-survival signal to rescue the remaining functional myocardium and reduce cell apoptosis and loss after the initial ischemic event [ 9 , 28 , 33 ]. Meanwhile, SP can mediate processes required for infarct repair, such as angiogenesis induction, more favorable ECM remodeling, and stem cell recruitment.…”

Section: Discussionmentioning

confidence: 99%

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In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

Shafiq

Zhang

Zhu

et al. 2018

Regenerative Biomaterials

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Cardiovascular diseases cause huge socio-economic burden worldwide. Although a mammalian myocardium has its own limited healing capability, scaffold materials capable of releasing stem cell recruiting/engrafting factors may facilitate the regeneration of the infarcted myocardium. The aim of this research was to develop cardiac patches capable of simultaneously eluting substance P (SP) and insulin-like growth factor-1C (IGF-1C) peptide. Polycaprolactone/collagen type 1-based patches with or without SP and IGF-1C peptide were fabricated by co-electrospinning, which exhibited nanofibrous morphology. SP and IGF-1C/SP patches recruited significantly higher numbers of bone marrow-mesenchymal stem cells than that of the negative control and patch-only groups in vitro. The developed patches were transplanted in an infarcted myocardium for up to 14 days. Mice underwent left anterior descending artery ligation and received one of the following treatments: (i) sham, (ii) saline, (iii) patch-only, (iv) IGF-1C patch, (v) SP patch and (vi) IGF-1C/SP patch. SP and IGF-1C/SP patch-treated groups exhibited better heart function and attenuated adverse cardiac remodeling than that of the saline, patch-only and individual peptide containing cardiac patches. SP patch and IGF-1C/SP patch-treated groups also showed higher numbers of CD31-positive vessels and isolectin B4-positive capillaries than that of other groups. IGF-1C/SP-treated group also showed thicker left ventricular wall in comparison to the saline and patch-only groups. Moreover, IGF-1C/SP patches recruited significantly higher numbers of CD29-positive cells and showed less numbers of Tunel-positive cells compared with the other groups. These data suggest that SP and IGF-1C peptides may act synergistically for in situ tissue repair.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

Shafiq

Zhang

Zhu

et al. 2018

Regenerative Biomaterials

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“…The incorporation of GSNO into the PL/CS hydrogel decreased the amount of total NO released by 20%, at the same period of time.It should be noted that for therapeutic purposes, a sustained and localized release of NO is desirable.Figure 4shows that the incorporation of GSNO into PL/CS hydrogel promotes a sustained NO release, in concentrations suitable for biomedical applications, for at least 24 h, at physiological and skin temperatures. In addition, the hydrogel allows an increase in residence time of the NO donor directly on the target site of application, making this formation good candidate for topical NO delivery system[1,7,[67][68][69].…”

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confidence: 99%

Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications

Pelegrino

Lima

Nascimento

et al. 2018

Preprint

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Nitric oxide (NO) is involved in physiological processes, including vasodilatation, wound healing and antibacterial activities. As NO is a free radical, designing drugs to generate therapeutic amounts of NO in controlled spatial and time manners is still a challenge. In this study, the NO donor S-nitrosoglutathione (GSNO) was incorporated into the thermoresponsive Pluronic F-127 (PL) - chitosan (CS) hydrogel, with an easy and economically feasible methodology. CS is a polysaccharide with known antimicrobial properties. Scanning electron microscopy, rheology and differential scanning calorimetry techniques were used for hydrogel characterization. The results demonstrated that the hydrogel has a smooth surface, thermoresponsive behavior and good mechanical stability. The kinetics of NO release and GSNO diffusion from GSNO-containing PL/CS hydrogel demonstrated a sustained NO/GSNO release, in concentrations suitable for biomedical applications. The GSNO-PL/CS hydrogel demonstrated a concentration-dependent toxicity to Vero cells, and antimicrobial activity to Pseudomonas aeruginosa (minimum inhibitory concentration and minimum bactericidal concentration values of 0.5 µg·mL-1 of hydrogel, which corresponds to 1 mmol·L-1 of GSNO). Interestingly, the concentration range in which the NO-releasing hydrogel demonstrated an antibacterial effect was not found to be toxic to the Vero mammalian cell. Thus, the GSNO-PL/CS hydrogel is a suitable biomaterial for topical NO delivery applications.

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“…8 Full-thickness skin substitutes are composed of both epidermal and dermal layers. Either autogenic or allogenic cells are used to generate the bioengineered construct.…”

Section: Introductionmentioning

confidence: 99%

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

Kumar¹

2017

ATROA

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Tissue engineered skin substitutes are used in chronic, partial or full thickness wounds, and serve to augment the growth and repair of the skin at the wound site, by acting as a temporary or permanent skin replacement. In this study Polycaprolactone a biodegradable polymer was electrospun in a binary solvent system to form nonwoven mats with porous fibers (PPCL). These mats were hydrolysed with alkali to make them more hydrophilic and promote cell adhesion. The electrospun mat served as a scaffold for culture of human keratinocyte (HaCaT) and rabbit hair follicle dermal fibroblast cells (HFDF) cells. F-actin staining and SEM analysis showed that the cells exhibited their characteristic morphology, with good adhesion and spreading. Live dead staining confirmed the viability of the cells on the PPCL mat. A bioengineered construct of HaCaT and HFDF cells cultured on either side of the PPCL mat was generated in vitro. Light microscopy of Toluidine blue stained cryo sections of the construct reveal that the HaCaT cells formed intact multilayers, while the fibroblasts showed a more distributed growth pattern with migration into the mat, hence emulating the epidermis and dermis of the skin. The results were substantiated in the SEM micrographs and fluorescent images of DiI and DiO labeled cocultures. The PPCL mat cocultured with HaCaT and HFDF cells thus finds potential as a self organized tissue engineered skin substitute..

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Functional poly(ε-caprolactone)/chitosan dressings with nitric oxide-releasing property improve wound healing

Cited by 130 publications

References 50 publications

In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

In situ cardiac regeneration by using neuropeptide substance P and IGF-1C peptide eluting heart patches

Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

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