Functional Outcome of Bone Marrow Stem Cells (CD45+/CD34−) After Cell Therapy in Chronic Spinal Cord Injury in Wistar Rats

Carvalho, Katherine Athayde Teixeira de; Vialle, Emiliano; Moreira, Guilherme Henrique Gonçalves; Cunha, Ricardo; Simeoni, Rossana Baggio; Francisco, Júlio César; Guarita-Souza, Luiz César; Oliveira, Leise Kelli de; Zocche, Larissa; Olandoski, Márcia

doi:10.1016/j.transproceed.2008.02.054

Cited by 18 publications

(9 citation statements)

References 3 publications

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“…Central nervous system injuries, such as SCI, results primarily from trauma, and can lead to loss of function and paralysis [17,18]. Unfortunately, SCI has no proper treatment.…”

Section: Discussionmentioning

confidence: 99%

Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation after Spinal Cord Injury in Rats

Alkarim¹,

Ramadan²,

Abdel-Hamid³

et al. 2018

Preprint

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Background: In spinal cord injury, radical treatment is still a persistent hope for patients and clinicians. Our study aimed to determine the different histological changes in central, cranial and caudal sites of compressed spinal cord as a result of neuroectodermal stem cells (NESCs) transplantation in rats. Material and methods: For extraction of NESCs, future brains were extracted from mice embryos (10-days old) and cultured.  Eighty, male rats were divided randomly into control, sham (20 rats each); while 40 rats were subjected to compressed spinal cord injury (CSCI). Seven days after spinal cord injury, rats were subdivided into 2 groups (20 rats each); an untreated and treated with NESCs injected cranial and caudal to the site of the spinal cord injury. Rats were sacrificed 4 weeks after transplantations of NESCs and specimens from the spinal cord at the central, cranial and caudal to site of spinal cord injury were proceeded to be stained with haematoxylin & eosin, osmic acid and Immunohistochemistry of glial fibrillary acidic protein (GFAP). Results: Sections of CSCI revealed areas of hemorrhages, necrosis and cavitation limited by reactive astrocytosis, with upregulation of GFAP expression. Evidence of remyelination and mitigation of histopathological features, reactive astrocytosis in CSCI sections were more pronounced in cranial than in caudal region. Conclusions: NESCs transplantation ameliorated the pathological changes, promoted remyelination.

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“…Central nervous system injuries, such as SCI, results primarily from trauma, and can lead to loss of function and paralysis [17,18]. Unfortunately, SCI has no proper treatment.…”

Section: Discussionmentioning

confidence: 99%

Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation after Spinal Cord Injury in Rats

Alkarim¹,

Ramadan²,

Abdel-Hamid³

et al. 2018

Preprint

View full text Add to dashboard Cite

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“…It has been reported that the G-CSF-mobilized MNC fraction is capable of neovascular regeneration in the ischemic brain (26). As for the spinal cord, recent reports have indicated that the G-CSF-mobilized MNC fraction can promote angiogenesis and has therapeutic effects in SCI (5), indicating that other cell populations than CD34-positive cells can promote neurological recovery.…”

Section: Discussionmentioning

confidence: 99%

Transplanted Peripheral Blood Stem Cells Mobilized by Granulocyte Colony-Stimulating Factor Promoted Hindlimb Functional Recovery after Spinal Cord Injury in Mice

et al. 2016

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Granulocyte colony-stimulating factor (G-CSF) mobilizes peripheral blood stem cells (PBSCs) derived from bone marrow. We hypothesized that intraspinal transplantation of PBSCs mobilized by G-CSF could promote functional recovery after spinal cord injury. Spinal cords of adult nonobese diabetes/severe immunodeficiency mice were injured using an Infinite Horizon impactor (60 kdyn). One week after the injury, 3.0 μl of G-CSF-mobilized human mononuclear cells (MNCs; 0.5 × 10 5 /μl), G-CSF-mobilized human CD34-positive PBSCs (CD34; 0.5 × 10 5 /μl), or normal saline was injected to the lesion epicenter. We performed immunohistochemistry. Locomotor recovery was assessed by Basso Mouse Scale. The number of transplanted human cells decreased according to the time course. The CD31-positive area was significantly larger in the MNC and CD34 groups compared with the vehicle group. The number of serotonin-positive fibers was significantly larger in the MNC and CD34 groups than in the vehicle group. Immunohistochemistry revealed that the number of apoptotic oligodendrocytes was significantly smaller in cell-transplanted groups, and the areas of demyelination in the MNC-and CD34-transplanted mice were smaller than that in the vehicle group, indicating that cell transplantation suppressed oligodendrocyte apoptosis and demyelination. Both the MNC and CD34 groups showed significantly better hindlimb functional recovery compared with the vehicle group. There was no significant difference between the two types of transplanted cells. Intraspinal transplantation of G-CSF-mobilized MNCs or CD34-positive cells promoted angiogenesis, serotonergic fiber regeneration/ sparing, and preservation of myelin, resulting in improved hindlimb function after spinal cord injury in comparison with vehicle-treated control mice. Transplantation of G-CSF-mobilized PBSCs has advantages for treatment of spinal cord injury in the ethical and immunological viewpoints, although further exploration is needed to move forward to clinical application.

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“…Spinal cord injury has become one of the main causes of morbidity and mortality. Current treatment methods including surgery, medication and physiotherapy have limited efficacy[1314151617181920212223242526272829]. In recent years, mesenchymal stem cell transplantation for the treatment of neurological diseases has shown considerable therapeutic potential[303132].…”

Section: Discussionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury

Zhang

Zhao

et al. 2014

Neural Regen Res

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We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model group. After 4 weeks, behavioral performance was significantly enhanced in the model group. After 8 weeks, the number of horseradish peroxidase-labeled nerve fibers was higher in the Nogo-66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and significantly higher than in the model group. The newly formed nerve fibers and myelinated nerve fibers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.

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Functional Outcome of Bone Marrow Stem Cells (CD45+/CD34−) After Cell Therapy in Chronic Spinal Cord Injury in Wistar Rats

Cited by 18 publications

References 3 publications

Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation after Spinal Cord Injury in Rats

Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation after Spinal Cord Injury in Rats

Transplanted Peripheral Blood Stem Cells Mobilized by Granulocyte Colony-Stimulating Factor Promoted Hindlimb Functional Recovery after Spinal Cord Injury in Mice

Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury

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