Functional Organization of hsp70 Cluster in Camel (Camelus dromedarius) and Other Mammals

Гарбуз, Д. Г.; Astakhova, L. N.; Zatsepina, Olga G.; Arkhipova, Irina R.; Nudler, Evgeny; Evgen’ev, Michael B.

doi:10.1371/journal.pone.0027205

Cited by 24 publications

(20 citation statements)

References 26 publications

(49 reference statements)

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“…In Brown Swiss cattle, increased HSP72 or HSPA1A mRNA levels in peripheral blood mononuclear cells due to heat stress have also been reported by Lacetera et al (2006). Our results were also supported by earlier studies in camel showing that temperature elevation increases the level of constitutively expressed HSP70 in camel (Garbuz et al 2011;Ulmasov et al 1993).…”

Section: Discussionsupporting

confidence: 91%

Seasonal variation in expression pattern of genes under HSP70

Banerjee

Upadhyay

Chaudhary

et al. 2014

Cell Stress and Chaperones

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Heat shock protein 70 (HSP70) is one of the most abundant and best characterized heat shock protein family that consists of highly conserved stress proteins, expressed in response to stress, and plays crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and to quantify their expression pattern in heat-and cold-adapted Indian goats (Capra hircus ) with respect to different seasons. Five HSP70 gene homologues to HSPA8, HSPA6, HSPA1A, HSPA1L, and HSPA2 were identified by gene-specific primers. The cDNA sequences showed high similarity to other mammals, and proteins have an estimated molecular weight of around 70 kDa. The expression of HSP70 genes was observed during summer and winter. During summer, the higher expression of HSPA8, HSPA6, and HSPA1A was observed, whereas the expression levels of HSPA1L and HSPA2 were found to be lower. It was also observed that the expression of HSPA1A and HSPA8 was higher during winter in both heat-and cold-adapted goats but downregulates in case of other HSPs. Therefore, both heat and cold stress induced the overexpression of HSP70 genes. An interesting finding that emerged from the study is the higher expression of HSP70 genes in cold-adapted goats during summer and in heat-adapted goats during winter. Altogether, the results indicate that the expression pattern of HSP70 genes is species-and breed-specific, most likely due to variations in thermal tolerance and adaptation to different climatic conditions.

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Section: Discussionsupporting

confidence: 91%

Seasonal variation in expression pattern of genes under HSP70

Banerjee

Upadhyay

Chaudhary

et al. 2014

Cell Stress and Chaperones

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“…In Hydra oligactis , the transcription of HSP70 can be induced by high temperature, but rapid degradation of HSP70 mRNA at elevated temperature negates this increase in transcription 93 . In other cases, hints about changes in the regulation of the canonical HSPs can be found in the arrangement of heat shock elements in their promoter regions 94, 95 or from the (re)arrangement of HSP genes within the genome that suggest that they are differentially responsive to stimuli 96 .…”

Section: Evolution Of the Proteostasis Networkmentioning

confidence: 99%

Diversity in the origins of proteostasis networks — a driver for protein function in evolution

Powers

Balch

2013

Nat Rev Mol Cell Biol

210

198

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Although a protein’s primary sequence largely determines its function, proteins can adopt different folding states in response to changes in the environment, some of which may be deleterious to the organism. All organisms, including Bacteria, Archaea and Eukarya, have evolved a protein homeostasis network, or proteostasis network, that consists of chaperones and folding factors, degradation components, signalling pathways and specialized compartmentalized modules that manage protein folding in response to environmental stimuli and variation. Surveying the origins of proteostasis networks reveals that they have co-evolved with the proteome to regulate the physiological state of the cell, reflecting the unique stresses that different cells or organisms experience, and that they have a key role in driving evolution by closely managing the link between the phenotype and the genotype.

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“…It was also shown that the total protein synthesis in camel cells is significantly more resistant to heat than that of human cells [ 18 ]. Recently, inducible and constitutive camel HSP70 genes have been sequenced in our laboratory and compared with orthologous genes from other mammalian species [ 19 ]. Herein, we compare the transcriptional activity of the camel and human HSP70 gene promoters by exploring a luciferase reporter in an in vitro assay in two different human cell lines.…”

Section: Introductionmentioning

confidence: 99%

Activity of Heat Shock Genes’ Promoters in Thermally Contrasting Animal Species

et al. 2015

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Heat shock gene promoters represent a highly conserved and universal system for the rapid induction of transcription after various stressful stimuli. We chose pairs of mammalian and insect species that significantly differ in their thermoresistance and constitutive levels of Hsp70 to compare hsp promoter strength under normal conditions and after heat shock (HS). The first pair includes the HSPA1 gene promoter of camel (Camelus dromedarius) and humans. It was demonstrated that the camel HSPA1A and HSPA1L promoters function normally in vitro in human cell cultures and exceed the strength of orthologous human promoters under basal conditions. We used the same in vitro assay for Drosophila melanogaster Schneider-2 (S2) cells to compare the activity of the hsp70 and hsp83 promoters of the second species pair represented by Diptera, i.e., Stratiomys singularior and D. melanogaster, which dramatically differ in thermoresistance and the pattern of Hsp70 accumulation. Promoter strength was also monitored in vivo in D. melanogaster strains transformed with constructs containing the S. singularior hsp70 ORF driven either by its own promoter or an orthologous promoter from the D. melanogaster hsp70Aa gene. Analysis revealed low S. singularior hsp70 promoter activity in vitro and in vivo under basal conditions and after HS in comparison with the endogenous promoter in D. melanogaster cells, which correlates with the absence of canonical GAGA elements in the promoters of the former species. Indeed, the insertion of GAGA elements into the S. singularior hsp70 regulatory region resulted in a dramatic increase in promoter activity in vitro but only modestly enhanced the promoter strength in the larvae of the transformed strains. In contrast with hsp70 promoters, hsp83 promoters from both of the studied Diptera species demonstrated high conservation and universality.

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Functional Organization of hsp70 Cluster in Camel (Camelus dromedarius) and Other Mammals

Cited by 24 publications

References 26 publications

Seasonal variation in expression pattern of genes under HSP70

Seasonal variation in expression pattern of genes under HSP70

Diversity in the origins of proteostasis networks — a driver for protein function in evolution

Activity of Heat Shock Genes’ Promoters in Thermally Contrasting Animal Species

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