Functional nucleic acids as modular components against SARS-CoV-2: From diagnosis to therapeutics

Zhang, Wenxian; Liu, Na; Zhang, Jing Jing

doi:10.1016/j.bios.2021.113944

Cited by 21 publications

(17 citation statements)

References 84 publications

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“… 60 , 61 Since early 2020, the relevant FNAs mentioned above have become ideal candidates for diagnosis and treatments against SARS-CoV-2 infection. Since there have already been several review articles surveying FNA-based virus detection, 7 , 8 , 9 we only focus on FNA-based therapeutics against virus infection in this review, because a review on this topic is lacking.…”

Section: An Overview Of Sars-cov-2: From Its Components and Symptoms ...mentioning

confidence: 99%

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Functional nucleic acids as potent therapeutics against SARS-CoV-2 infection

Chen¹,

Liu²,

Liu³

2023

Cell Reports Physical Science

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Section: An Overview Of Sars-cov-2: From Its Components and Symptoms ...mentioning

confidence: 99%

“…Although several review articles with emphasis on the diagnosis of SARS-CoV-2 via FNAs have been published, 7 , 8 , 9 a comprehensive review on FNAs toward SARS-CoV-2 treatment is apparently lacking. To bridge such a gap, we survey the recent development of FNAs as potent therapeutics against SARS-CoV-2 infection in this review.…”

Section: Introductionmentioning

confidence: 99%

Functional nucleic acids as potent therapeutics against SARS-CoV-2 infection

Chen¹,

Liu²,

Liu³

2023

Cell Reports Physical Science

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“…There are urgent needs and tremendous opportunities for the development of alternative diagnostic techniques, especially for POCT methods [156]. To date, a considerate number of aptamers have been selected for diagnostic and therapeutic purposes toward COVID-19, which are ideal candidates to be functionalized as recognition moieties in corporation with versatile POCT techniques [157].…”

Section: Covid-19mentioning

confidence: 99%

Aptasensors for the detection of infectious pathogens: design strategies and point-of-care testing

2022

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The epidemic of infectious diseases caused by contagious pathogens is a life-threatening hazard to the entire human population worldwide. A timely and accurate diagnosis is the critical link in the fight against infectious diseases. Aptamer-based biosensors, the so-called aptasensors, employ nucleic acid aptamers as bio-receptors for the recognition of target pathogens of interest. This review focuses on the design strategies as well as state-of-the-art technologies of aptasensor-based diagnostics for infectious pathogens (mainly bacteria and viruses), covering the utilization of three major signal transducers, the employment of aptamers as recognition moieties, the construction of versatile biosensing platforms (mostly micro and nanomaterial-based), innovated reporting mechanisms, and signal enhancement approaches. Advanced point-of-care testing (POCT) for infectious disease diagnostics are also discussed highlighting some representative ready-to-use devices to address the urgent needs of currently prevalent coronavirus disease 2019 (COVID-19). Pressing issues in aptamer-based technology and some future perspectives of aptasensors are provided for the implementation of aptasensor-based diagnostics into practical application.

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“…Consequently, quick and precise identification and monitoring of coronavirus infection are crucial for preventing disease transmission and ultimately saving lives [4][5][6][7]. Generally, there are two diagnostic methods for SARS-CoV-2, serological and viral nucleic acid tests [8][9][10][11]. Serological testing detects the presence of antibodies or the antigenic viral proteins produced by an (infected) individual because of exposure to the virus.…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant

Chen

et al. 2022

Nano-Micro Lett.

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Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now, which is time-consuming and requires expensive instrumentation, and the confirmation of variants relies on further sequencing techniques. Herein, we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid, highly sensitivity and specificity for the detection of SARS-CoV-2 variant. To enhance the sensing capability, gold electrodes are uniformly decorated with electro-deposited gold nanoparticles. Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model, our biosensor exhibits excellent analytical detection limit (50 fM) and high linearity (R2 = 0.987) over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays. The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system. Furthermore, based on the wireless micro-electrochemical platform, the proposed biosensor reveals promising application ability in point-of-care testing.

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Functional nucleic acids as modular components against SARS-CoV-2: From diagnosis to therapeutics

Cited by 21 publications

References 84 publications

Functional nucleic acids as potent therapeutics against SARS-CoV-2 infection

Functional nucleic acids as potent therapeutics against SARS-CoV-2 infection

Aptasensors for the detection of infectious pathogens: design strategies and point-of-care testing

CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant

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