Functional modulation of caecal fermentation and microbiota in rat by feeding bean husk as a dietary fibre supplement

Abstract

A feeding study using rats was conducted to evaluate the utility of lablab bean husk and soya bean husk as sources of potential prebiotic fibre. Twenty 5-week-old Sprague Dawley rats were divided into 4 groups and fed one of the following diets for 3 weeks: purified diet (AIN93 G) containing 5% cellulose (CEL), or the same diet in which cellulose was replaced by corn starch (STA), lablab bean husk (LBH), or soya bean husk (SBH). Rats were sacrificed at 8 weeks of age and caecal digesta were collected. Feed intake, body weight, anatomical parameters, and caecal ammonia level did not differ significantly among diets. Rats on LBH and SBH showed higher concentrations of caecal short-chain fatty acid and lactate than those on CEL. Rats on CEL, SBH, and LBH exhibited lower caecal indole and skatole levels. LBH yielded increased caecal abundance of Akkermansia muciniphila and Oscillibacter relatives, as demonstrated by either qPCR, MiSeq, or clone library analysis. SBH favoured the growth of lactobacilli as assessed by both qPCR and MiSeq, and favoured the growth of bifidobacteria as assessed by MiSeq. In comparison with STA, LBH and SBH yielded lower caecal abundance of bacteria related to Dorea massiliensis, as demonstrated by qPCR, MiSeq, and clone library analysis. Both types of bean husk were found to contain oligosaccharides that might selectively stimulate the growth of beneficial bacteria. Based on these results, the two species of bean husk tested are considered potentially functional for promoting the gut health of monogastric animals.

Soybean oligosaccharide, stachyose, and raffinose in broilers diets: effects on odor compound concentration and microbiota in cecal digesta

“…The inclusion of 3.5 to 5.0 g/kg of soybean oligosaccharide to the diet had a decreasing effect on the excreta skatole of broilers ( Li, 2018 ), and except for soybean oligosaccharides, the addition of stachyose, raffinose, and saccharose also decreased the concentrations of skatole in broiler cecal microbial broth ( Yang et al., 2017 ). The results of the study by Myint et al. (2018) suggested that feeding bean husk as a dietary fiber supplement (containing 0.1% stachyose and 0.1% raffinose in carbohydrate) exhibited lower skatole and indole levels in rat cecum.…”

The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function

“…A breakdown of epithelial integrity is found in patients with irritable bowel disease (IBD) where butyrate producing bacteria are specifically reduced when compared to healthy subjects [ 61 ]. Relative to the control group, Oscillibacter abundance was increased in pigs fed both three and six servings of WB mushrooms similar to animals fed diets rich in fermentable polysaccharides and cellulosic feeds [ 62 , 63 , 64 ]. Butyricicoccus, another producer of butyrate, has been purported to strengthen epithelial barrier function [ 65 ] and has improved good hygiene in pigs [ 66 ].…”

The Role of Short-Chain Fatty Acids of Gut Microbiota Origin in Hypertension

“…A high-salt diet alters the production of SCFAs by disrupting the composition of gut microbiota; thus, hypertension develops ( Bier et al, 2018 ). Meanwhile, a high-fibre diet and dietary fibre supplementation could increase the circulating levels of SCFAs and subsequently reduce blood pressure ( Myint et al, 2018 ; Zhai et al, 2018 ; Zhao et al, 2018 ). In an experiment using a hypertensive rat model, Marques et al indicated that the systolic and diastolic pressure levels were significantly lower in DHR with high-fibre diet or acetate supplementation than in the control group ( Marques et al, 2017 ).…”