Functional mapping of human sound and carious enamel and dentin with Raman spectroscopy

Abstract

The goals of this trial were, first, to produce a Raman mapping of decay and sound dentin samples, through accurate analysis of the Raman band spectra variations of mineral and organic components. The second goal was to confirm the correlation between the Raman signal and the signal of a fluorescent camera, by assaying the concentration of pentosidine and natural collagen fluorescent crosslink using reverse phase high-pressure liquid chromatography. The first correlation assumed a possible relationship between the signal observed with the camera and Raman spectroscopy. The second correlation assumed an association with the Maillard reaction. Absence of a correlation for this trial was that no association could be found between Raman spectra characteristics, fluorescence variation and the HPLC assay. Our results void this absence.

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

“…Similarly, Raman analysis also exhibited an increase of collagen crosslinking at the hybrid layer and dentin (Table 4), conducting to a less mineralized (Schwartz et al, 2012) but more mature tissue (Sell and Monnier, 1989), in comparison with dentin treated with Zn-NPs/24 h or Ca-NPs/21 d. Collagen crosslinking is affected by the tissue maturation as well as the degree of mineralization, providing information about the structure and molecular interactions of complexes biomolecules (Saito et al, 2006;Xu and Wang, 2011). Pentosidine, is the main AGE (advance glycation end products) component (Salehi et al, 2013). Its presence strongly suggest ribose or ribonucleotide metabolites as precursors (Sell and Monnier, 1989).…”

“…Pentosidine has been demonstrated to contribute to the age-related stiffening of tissues by cross-linking of the extracellular matrix, and may serve as a molecular marker of the aging process (Sell and Monnier, 1989). The higher intensity signal at the interface indicates greater potential for further remineralization and scaffolding (Salehi et al, 2013), considering that both hybrid layer and dentin resulted in a more immature substrate (increased CH2 peak) (Table 4) but with better organization of collagen for promoting apatite nucleation (increased ratio A-I/A-III) (Table 4) (Xu and Wang, 2012). After 24 h of SBFS storage, some demineralized collagen fibers were patent at the bottom of the Zn-NPs-infiltrated hybrid layer, reflecting this lack of complete remineralization ( Figures 2E, 2F).…”

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

“…This difference also denotes a greater sensitivity to molecular orientation in order to enhance further crystallization. From a clinical standpoint, recovery [51] , better organization [52] , and collagen quality [53] [34] followed similar trend (Table 4). The amide A band, at 3396 cm -1 showed a 2.15 cm -1 (sound dentin) and 7.05 cm -1 (caries-affected dentin) shift to high frequencies, respectively, when Zn-containing amalgams were employed (Table 4).…”

Detection of Occlusal Carious Lesion using the SoproLife® Camera: A Systematic Review

“…Of the 11 articles originally identified with the search keyword, [13][14][15][16][17][18][19][20][21][22][23] only three articles met all the inclusion criteria and were retained for this review.…”

“…Two surveys were undertaken only on permanent teeth 14,15 and one took also primary teeth into account. 16 All the three included surveys used the ICDAS-II criteria [17][18][19] as reference for the assessment of tested devices which were the SoproLife ® , 15 SoproLife ® compared to DIAGNOdent ® and Spectra Caries Detection Aid ®14 or to DIAGNOdent pen ® . 16 The ICDAS-II scoring Table 3 presents the values of the validity-related parameters reported in the three included articles.…”