Functional Magnetic Resonance Imaging of Rats with Experimental Autoimmune Encephalomyelitis Reveals Brain Cortex Remodeling

Tambalo, Stefano; Peruzzotti-Jametti, ﻿Luca; Rigolio, R; Fiorini, Silvia; Bontempi, Pietro; Mallucci, Giulia; Balzarotti, Beatrice; Marmiroli, P; Sbarbati, Andrea; Cavaletti, Guido; Pluchino, ﻿Stefano; Marzola, Pasquina

doi:10.1523/jneurosci.0540-15.2015

Cited by 55 publications

(46 citation statements)

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“…Recently, in a fMRI study, Tambalo et al investigated the functional response to somatosensory stimulation of the forepaw in DA Dark Agouti rats with experimental autoimmune encephalomyelitis (EAE), a widely accepted preclinical model of chronic multiple sclerosis (MS). 32 Alterations of the functional response of EAE mice (in the relapsing and chronic phases) were found and this finding was in line with human fMRI studies showing that cortical reorganization occurs in MS patients. 33 These results provide additional support to the validity of the DA EAE rat model for fMRI studies and for the development of innovative therapies in EAE/MS research.…”

Section: Introductionsupporting

confidence: 82%

“…33 These results provide additional support to the validity of the DA EAE rat model for fMRI studies and for the development of innovative therapies in EAE/MS research. 32 In animals, fMRI can also be performed using exogenous paramagnetic or superparamagnetic contrast agents. When confined in the vascular space, they create a strong inhomogeneity in the static magnetic field and consequently a decrease in signal intensity.…”

Section: Introductionmentioning

confidence: 99%

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In vivo imaging techniques: a new era for histochemical analysis

et al. 2016

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In vivo imaging techniques can be integrated with classical histochemistry to create an actual histochemistry of water. In particular, Magnetic Resonance Imaging (MRI), an imaging technique primarily used as diagnostic tool in clinical/preclinical research, has excellent anatomical resolution, unlimited penetration depth and intrinsic soft tissue contrast. Thanks to the technological development, MRI is not only capable to provide morphological information but also and more interestingly functional, biophysical and molecular. In this paper we describe the main features of several advanced imaging techniques, such as MRI microscopy, Magnetic Resonance Spectroscopy, functional MRI, Diffusion Tensor Imaging and MRI with contrast agent as a useful support to classical histochemistry.

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Section: Introductionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

In vivo imaging techniques: a new era for histochemical analysis

et al. 2016

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“…Wu et al [35] measured microvascular volume distribution in mice using a 30 mgFe/kg injection at 9.4 T and demonstrated that CBV in the cerebral cortex is higher in transgenic APP mice, an experimental model of Alzheimer's disease, than in controls. CBV-weighted functional MRI (fMRI) has been extensively used in experimental fMRI [36,37]; thanks to the increased sensitivity of CBV-weighted contrast compared with classical blood oxygen level-dependent (BOLD) contrast. At 7 T, 15-20 mg Fe/kg ultrasmall superparamagnetic iron oxides improve functional contrast-to-noise ratio (CNR) by 70% in rats compared with BOLD contrast [38].…”

Section: Discussionmentioning

confidence: 99%

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver

et al. 2017

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Aim:To study the efficiency of multifunctional polymer-based superparamagnetic iron oxide nanoparticles (bioferrofluids) as a T 2 magnetic resonance contrast agent and their uptake and toxicity in liver. Materials & methods: Mice were intravenously injected with bioferrofluids and Endorem R . The magnetic resonance efficiency, uptake and in vivo toxicity were investigated by means of magnetic resonance imaging (MRI) and histological techniques. Results: Bioferrofluids are a good T 2 contrast agent with a higher r 2 /r 1 ratio than Endorem. Bioferrofluids have a shorter blood circulation time and persist in liver for longer time period compared with Endorem. Both bioferrofluids and Endorem do not generate any noticeable histological lesions in liver over a period of 60 days post-injection. Conclusion: Our bioferrofluids are powerful diagnostic tool without any observed toxicity over a period of 60 days post-injection.Lay abstract: Several superparamagnetic iron oxide nanoparticles (SPIONs) preparations have been approved by US FDA for clinical use as MRI contrast agents. In recent years, we have been developing a synthetic multifunctional platform for SPIONs based on the use of polymers. In this report, we explored the diagnostic potential of these nanoparticles (herein called bioferrofluids) as an MRI contrast agent and their uptake in liver, without neglecting their toxicological effects. Results show that our bioferrofluids are a good T 2 contrast agent without any observed toxicity in liver.

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“…Pre-processing of data included: (i) export into DICOM format from ParaVision 6.0.1 (Bidgood Jr et al, 1997); (ii) conversion into NifTI using the dcm2niix converter (64-bit Linux version 5 May 2016) (Rorden et al, 2007); (iii) reorienting the brain into leftanterior-superior (LAS) axes (radiological view); and (iv) skull-stripping using the qimask utility from QUIT (QUantitative Imaging Tools) (Wood, 2018). The voxel sizes were then upscaled by a factor of 10 (Tambalo et al, 2015).…”

Section: Rs-fmri Datamentioning

confidence: 99%

Validation of chronic restraint stress model in rats for the study of depression using longitudinal multimodal MR imaging

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Feindel

et al. 2020

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Prior research suggests that the neurobiological underpinnings of depression include disruptions in functional connectivity, neurometabolite levels, and hippocampal volume. This study examined the validity of a chronic restraint stress (CRS) paradigm in male Sprague Dawley rats for the study of depression using longitudinal behavioural tests and multiple 9.4 T MRI modalities (resting-state functional MRI, proton magnetic resonance spectroscopy, and volumetric studies). In the CRS protocol, rats were placed in individual transparent tubes for 2.5 h daily over 13 days. Elevated plus-maze test (EPM) and forced swim test (FST) confirmed the presence of anxiety-like and depression-like behaviours respectively post-restraint. Brain changes were also detected by MR. The rs-fMRI data revealed hypoconnectivity within the salience and interoceptive networks and hyperconnectivity of several brain regions to the cingulate cortex. The 1 H-MRS data revealed decreased sensorimotor cortical glutamate, glutamine and combined glutamate-glutamine levels. Volumetric analysis of T2-weighted images revealed decreased hippocampal volume, which was also correlated with salience network connectivity. Depression-like behaviours were correlated with salience and interoceptive network connectivity, glutamate and combined glutamate-glutamine levels and hippocampal volume. Anxiety-like behaviours were correlated with both hippocampus connectivity and interoceptive network connectivity. The present findings identify significant changes in brain connectivity, neurometabolites and structure that are correlated with abnormal behaviour in CRS rats. Importantly, these changes parallel those found in human depression, suggesting that the CRS rodent model has utility for translational studies and novel intervention development for depression.

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Functional Magnetic Resonance Imaging of Rats with Experimental Autoimmune Encephalomyelitis Reveals Brain Cortex Remodeling

Abstract: Cortical reorganization occurring in multiple sclerosis (MS) patients is thought to play a key role in limiting the

Cited by 55 publications

References 59 publications

In vivo imaging techniques: a new era for histochemical analysis

In vivo imaging techniques: a new era for histochemical analysis

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver

Validation of chronic restraint stress model in rats for the study of depression using longitudinal multimodal MR imaging

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Functional Magnetic Resonance Imaging of Rats with Experimental Autoimmune Encephalomyelitis Reveals Brain Cortex Remodeling

Abstract: Cortical reorganization occurring in multiple sclerosis (MS) patients is thought to play a key role in limiting the

Cited by 55 publications

References 59 publications

In vivo imaging techniques: a new era for histochemical analysis

In vivo imaging techniques: a new era for histochemical analysis

Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T 2 Contrast Agent for MRI and their Uptake in Liver

Validation of chronic restraint stress model in rats for the study of depression using longitudinal multimodal MR imaging

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Product

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Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as T ₂ Contrast Agent for MRI and their Uptake in Liver