Functional interactions of viral and histocompatibility antigens at tumor cell surfaces.

Schrader, John W.; Cunningham, Bruce A.; Edelman, Gerald M.

doi:10.1073/pnas.72.12.5066

Cited by 153 publications

(63 citation statements)

References 22 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the "altered self" concept, target cells are recognized because of a virus-induced alteration in H-2 antigens. Virus infections could alter H-2 antigens by direct interaction between H-2 and viral antigens (6)(7)(8)19) or indirectly, either by affecting H-2 antigen biosynthesis (6) or by depression of normally silent genes located within the H-2 major histocompatibility complex (20). The results presented in this paper indicate that cytotoxic T lymphocytes are capable of distinguishing target cells infected with related strains of influenza virus.…”

Section: Resultsmentioning

confidence: 57%

Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens.

Ennis¹,

Wj²,

Verbonitz³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Cytotoxic thymus-derived (T) lymphocytes were readily detected in spleens of mice inoculated intranasally with mouse-adapted A/Port Chalmers (H3N2), A/England (H3N2), A/PR/8 (H0n1), and B/Hong Kong influenza viruses. T-cell-mediated lysis of H-2 compatible target cells infected with the strain of virus used to immunize the mice was considerably higher than lysis of either syngeneic cells infected with a different strain of influenza virus or allogeneic cells infected with the immunizing strain of influenza virus. The findings that cytotoxic lymphocytes can distinguish minor antigenic variants among influenza viruses and that lysis depends on H-2 histocompatibility between lymphocyte and target cell support the concept of dual recognition of visual and H-2 histocompatibility antigens in T-cell-mediated antiviral immunity.

show abstract

Section: Resultsmentioning

confidence: 57%

Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens.

Ennis¹,

Wj²,

Verbonitz³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Would molecules adjacent to the modulated one show an increased expression, or would they "comodulate"? Since redistribution of antigens on crosslinking with bivalent antibodies precedes the modulation pro-cess, it may be that surrounding antigens become redistributed too, a phenomenon comparable to co-capping (Schrader et al, 1975). Old et al (1968) were able to show that antigenic modulation of TL antigens leads to an increased expression of H-2D antigens, which are themselves nonmodulable.…”

mentioning

confidence: 99%

Antigenic modulation of mammary tumour virus envelope antigen or GR thymic lymphoma cells in relation to expression of H-2, TL cell-surface antigens and THY1

Hilgers¹,

Sonnenberg²,

Nusse³

1980

Br J Cancer

View full text Add to dashboard Cite

The MLr antigen, a mammary tumour virus-induced antigen on the surface of GR thymic lymphoma cells (GRSL) can be modulated from the cell surface upon incubation with specific antiserum for 1-2 h at 37 degrees C, followed by washing the cells. In contrast, a number of other cell-surface antigens on these GRSL cells cannot be modulated under similar conditions. These antigens include histocompatibility antigens of the H-2 complex (H-2.8 of the K-end and H-2dx(D) of the H-2dx haplotype) and two thymic markers, TL1.2 and Thy1.2. Antigenic modulation of MLr as tested by trypan-blue exclusion and by chromium51 release does not lead to a measurable change in the expression of H-2K, H-2D, TL and Thy1.2 antigens. These results could be confirmed by absorption analysis. The latter analysis showed that the number of antigenic sites per cell are about the same for MLr and the two H-2 antigens, while TL antigens are scarcer and Thy1.2 antigens are more abundant. The procedure of antigenic modulation showed that the MLr antigen resides on MTVgp52, the major protein of the envelope. There was no evidence of internal proteins, such as MTVp27, on the surface of GRSL cells.

show abstract

“…The TSA could be spatially linked with the HLA antigens but on independently coded molecules with the HLA molecules serving as adaptors that combine with anitigens on the cell surface to form hybrid antigens containing elements of self (HLA) and non-self (TSA) (Ohno, 1977;Schrader, Cunningham and Edelman, 1975). In this context, however, a preliminary examination of our breast-cancer tumoutr isolates for associated murine mammary-tumour viral antigens have given negative results;* 3.…”

Section: Discussionmentioning

confidence: 99%

Isolation of human tumour-specific antigens associated with β2 microglobulin

Thomson¹,

Rauch²,

Friedlander³

et al. 1978

Br J Cancer

View full text Add to dashboard Cite

Summary.-In the present study the tube LAI assay was used to monitor the isolation of the TSA of 4 different types of human cancers. Each tumour antigen was found to be specific for tumours arising in the organ from which the TSA was initially derived and which were histopathologically similar. Immunochemical studies revealed that these molecules co-isolate with normal human HLA antigens and are associated with fl2m. On Sephadex G-150, the majority of the papain-solubilized tumour antigen eluted in the mol. wt range 70,000-150,000. Analysis of this material by SDS-PAGE and 6M guanidine-HCl column chromatography indicated that the material is composed of smaller subunits with prominent peaks at 40,000, 25,000 and 12,000 mol. wt. Immunoadsorbent affinity chromatography of the solubilized tumour-membrane constituents on AH-Sepharose-linked horse anti-human-fl2m indicated that the tumour antigens, like HLA molecules, contain a /32m subunit. The specificity of binding of TSA to the immunoadsorbent columns and the immunologically specific abrogation of LAI reactivity were clearly shown. The present study, therefore, indicates that by the isolation of fl2m, human tumour antigens can also be Isolated, since human tumour antigens are associated with fl2m. Whether human TSAs may perhaps be modified histocompatibility antigens remains to be answered. Although the change upon malignant transformation in the pattern of the cell-surface proteins expressing the TSA determinant remains obscure, it would appear that for tumours arising within a given organ, a consistent alteration of cell-surface proteins occurs.

show abstract

Functional interactions of viral and histocompatibility antigens at tumor cell surfaces.

Abstract: ABSTRACT

Cited by 153 publications

References 22 publications

Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens.

Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens.

Antigenic modulation of mammary tumour virus envelope antigen or GR thymic lymphoma cells in relation to expression of H-2, TL cell-surface antigens and THY1

Isolation of human tumour-specific antigens associated with β2 microglobulin

Contact Info

Product

Resources

About