Functional interaction between the early viral proteins of simian virus 40 and adenovirus

Goldman, Neil D.; Howley, Peter M.; Khoury, George

doi:10.1016/0042-6822(81)90501-8

Cited by 10 publications

(2 citation statements)

References 29 publications

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“…Furthermore, the defect in the DNA replication of the adenovirus DBP mutant Ad5tsl25 at the nonpermissive temperature can be partially overcome by coinfection with WT, but not a T antigen tsA mutant of SV40. In return, coinfection with Ad2 helps SV40 tsA down-regulate the expression of its early genes (29,55).…”

Section: Discussionmentioning

confidence: 99%

The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication

Rice

Klessig

1984

J Virol

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The adenovirus type 2 (Ad2) host range mutant Ad2hr400 grows efficiently in cultured monkey cells at 37°C, but is cold sensitive for plaque formation and late gene expression at 32.5°C. After nitrous acid mutagenesis of an Ad2hr400 stock, cold-resistant variants were selected in CV1 monkey cells at 32.5°C. One such variant, Ad2ts400, was also temperature sensitive (ts) for growth in both CV, and HeLa cells. Marker rescue analysis has been used to show that the two phenotypes, cold resistant and temperature sensitive, are due to two independent mutations, each of which resides in a different segment of the gene encoding the 72-kilodalton DNA binding protein (DBP). The cold-resistant mutation (map coordinates 63.6 to 66) is a host range alteration that enhances the ability of the virus to express late genes and grow productively in monkey cells at 32.5°C. The temperature-sensitive mutation is in the same complementation group and maps to the same segment of the DBP gene (map coordinates 61.3 to 63.6) as the well-characterized DBP mutant Ad5tsl25. Like Ad5tsl25, Ad2ts400 is unable to replicate viral DNA or to properly shut off early mRNA expression at the nonpermissive temperature. Two sets of experiments with Ad2ts400 suggest that DBP contains separate functional domains. First, when CV1 cells are coinfected at the nonpermissive temperature with Ad2 plus Ad2ts400 (Ad2 allows DNA replication and entry into, but not completion of, the late phase of infection), normal late gene expression and productive growth occur. Second, temperature shift experiments show that, although DNA replication is severely restricted at the nonpermissive temperature in ts400-infected monkey cells, late gene expression occurs normally. These results indicate that the DBP activity required for normal late gene expression in monkey cells is functional even when the DBP's DNA replication activity is disrupted.

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Section: Discussionmentioning

confidence: 99%

The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication

Rice

Klessig

1984

J Virol

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“…These cells, however, are nonpermissive for wild-type human adenoviruses, although their DNA is replicated almost as efficiently as in human cells (Delsert & D'Halluin, 1984). Superinfection of SV40-infected CV1 cells with H2WT results in a strong inhibition of SV40 DNA replication (Patch et al, 1981;Friedman et al, 1970;Goldman et al, 1981 ;van Roy & Fiers, 1978 ;and Fig. 3 b).…”

Section: Double Injection Of Simian Cells By Sv40 and Different Adenomentioning

confidence: 99%

An Adenovirus Cytocidal Function Related to the Control of a Cellular pH 4 Endonuclease Activity

D'Halluin

Delsert

Milleville

et al. 1985

Journal of General Virology

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SUMMARYAn adenovirus (Ad) interserotypic recombinant (H2cytl41) between temperaturesensitive mutant H2tsl 11 of Ad2 and deletion mutant H5d1313 of Ad5 was isolated and characterized. It was phenotypically ts +, dl ÷, hr + and formed large plaques (or cytocidal: cyt). It contained the right 89 % of Ad5 DNA and the leftmost 11% of Ad2 DNA. Genetic recombination data suggested the cytocidal mutation lay in the transforming region E1B, confirming sequence analysis. The cytocidal effect resulted in part from the breakdown of cellular DNA. Host cell and virus DNA breakdown induced by H2cytl41 appeared cell-dependent: it occurred in HeLa, KB or BHK-21 cells, but not in CV1 or 293 cells. In human cells the cyt effect was recessive and adenovirus DNA degradation was prevented by co-infection with adenovirus wildtype (H2WT), other adenovirus serotypes or simian virus 40 (SV40). In simian cells, H2cytl41 did not inhibit SV40 DNA replication, unlike H2WT. The amount of H2cytl41 DNA integrated in human cell DNA at early stages of the lytic cycle was found to be significantly lower than for H2WT. Novobiocin inhibited viral DNA breakdown in human cells. Cellular DNA extracted from H2cytl41-infected cells exhibited a repeat band pattern in gel electrophoresis reminiscent of the nuclease digestion pattern of chromatin, with monosome-size fragments as the digestion limit. The H2cyt141-induced nucleolytic effect would therefore occur in the linker regions of cell DNA and might result from the observed stimulation (by a factor of greater than 100) of an acidic (optimum pH 4.0) endonuclease activity. The nucleolytic effect also appeared to be recessive in vitro and absent in mixed samples containing extracts from H2cyt141-infected cells plus extracts from H2WT-or mock-infected cells. The virus gene product responsible for the enhancement of the acidic endonuclease was found to function stoichiometrically and not catalytically. The cytocidal and nucleolytic effects of the viral E1B region 19K protein may be mediated by a cellular inhibitor of acidic endonuclease.

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Replication of SV40 and Polyoma Virus Chromosomes

DePamphilis¹,

Bradley²

1986

The Papovaviridae

136

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Functional interaction between the early viral proteins of simian virus 40 and adenovirus

Cited by 10 publications

References 29 publications

The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication

The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication

An Adenovirus Cytocidal Function Related to the Control of a Cellular pH 4 Endonuclease Activity

Replication of SV40 and Polyoma Virus Chromosomes

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