Functional In Vivo Imaging of Tumors

Haris, Mohammad; Nisar, Sabah; Hashem, Sheema; Bhat, Ajaz A.; Yadav, Santosh K.; Shanmugakonar, Muralitharan; Al‐Naemi, Hamda; Bagga, Puneet; Uddin, Shahab; Reddy, Ravinder

doi:10.1007/978-3-030-38862-1_1

Cited by 1 publication

(1 citation statement)

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“…Visual biopsy tools have been gaining medical interest due to their simplicity and practicality (14). By allowing visualization of fresh, unprocessed and in vivo tissue, MPM acts as a complementary technology to the GSH.…”

Section: Prostate Cancer (Pca)mentioning

confidence: 99%

Value of multiphoton microscopy in uro-oncology: a narrative review

Treacy¹,

Khosla²,

Kyprianou³

et al. 2023

Transl Androl Urol

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Background and Objective: Multi-photon microscopy (MPM) is a 3-dimension fluorescence imaging technique that combines the excitation of two low-energy photons, enabling less photo-bleaching and deeper penetration of the imaged tissue. Two signals are detected, autofluorescence (AF), from natural intracellular fluorophores [such as nicotinamide adenine dinucleotide phosphate (NADP) and flavine adenine dinucleotide (FAD) transformation], and second harmonic generation (SHG), a physical property of the laser enhancing non-centrosymmetric structures such as collagen fibers. MPM can give both visual and quantitative information of a fresh tissue (without the need of processing, cutting or staining the tissue), aiding in the progress towards optimizing a real-time imaging device. The objective of this review is to show the value and benefits of the use of MPM in uro-oncology.Methods: A structured literature review was performed using PubMed and Web of Sciences, including all articles with the following keywords: "multiphoton microscopy", "two-photon microscopy", "non-linear microscopy", "second harmonic generation", "urology", "prostate", "bladder", "kidney", "upper tract", "oncology", "surgical margins", "frozen section". Articles were reviewed to summarize the use of this tool in performing biopsies, assessing surgical margins, staging and grading complementary tool, and real-time imaging.

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Section: Prostate Cancer (Pca)mentioning

confidence: 99%