Functional Implications of CD34 Expression in Human Adipose–Derived Stem/Progenitor Cells

Suga, Hirotaka; Matsumoto, Daisuke; Eto, Hikaru; Inoue, Keita; Aoi, Noriyuki; Kato, Harunosuke; Araki, Jun; Yoshimura, Kotaro

doi:10.1089/scd.2009.0003

Cited by 190 publications

(190 citation statements)

References 39 publications

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“…The CD34-positive cells in SVF are localized in the blood vessels in adipose tissue, and their staining is different from that of CD31 in that CD34-positive cells are visible in the adventitia of all blood vessels (16). Some data indicate that CD34-positive SVF cells have a higher proliferation rate than the CD34-negative cells (27). Another study showed that the CD34-positive cell population from cultured SVF (but not CD34-negative cells) was able to produce lipid-droplet formation activity under adipogenic culture conditions (18).…”

Section: Discussionmentioning

confidence: 99%

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

et al. 2015

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Our objective was to explore the mechanism of cell-assisted adipose transplantation by using freshly isolated human stromal vascular fraction (SVF) cells and to observe the dynamic changes of the graft after transplantation. The SVF was isolated from human liposuction aspirates, and 0.5 ml adipose tissue was mixed with 1 × 10 6 SVF cells or culture medium then injected to nude mice subcutaneously. At 1,4,7,14,30, 60, and 90 days after transplantation, samples were harvested for 1) general observation and retention rate; 2) whole-mount stain; 3) H&E stain; 4) immunohistochemical staining for S100, CD68, and CD34; 5) ELISA for VEGF and bFGF; 6) peroxisome proliferator-activated receptor-g (PPARg) fluorescence in situ hybridization. The retention rate in the experiment group was markedly higher than that in the control group. Whole-mount stain shows most of the cells in the center of the graft could not survive the ischemia until day 14. Histology showed new vessels on the surface of the graft at 3 days. However, in the control group, fewer newly formed vessels were detected until day 7. In the late stage of transplantation, gradual fibrosis was found in the graft, and the tissue was divided into a grid-like structure. A large number of round neonatal adipocytes with big nuclei in the center were found surrounding the new vessels, which were S100 and CD34 positive and CD68 negative. In the late stage of transplantation, most of the neonatal adipocytes were human PPARg positive. Moreover, the SVF group showed a higher level of VEGF and bFGF. SVF assisting adipose transplantation could increase the retention rate of the graft through promoting adipose tissue regeneration via secretion of growth factors, promotion of angiogenesis, and increasing the density of mesenchymal stem cells.

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Section: Discussionmentioning

confidence: 99%

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

et al. 2015

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“…Although generally considered to be a marker of haematopoietic progenitor cells, it is now agreed that there is positive CD34 expression on a subset of freshly isolated ASCs. 44,45 It has been reported that ASCs lose the expression of CD34 within 3 passages in tissue culture. This tissue culture phenomenon, has been largely unrecognized until recently.…”

Section: Discussionmentioning

confidence: 99%

Human cytomegalovirus infection of human adipose-derived stromal/stem cells restricts differentiation along the adipogenic lineage

et al. 2015

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Human adipose-derived stromal/stem cells (ASCs) display potential to be used in regenerative stem cell therapies and as treatments for inflammatory and autoimmune disorders. Despite promising use of ASCs as therapeutics, little is known about their susceptibility to infectious agents. In this study, we demonstrate that ASCs are highly susceptible to human cytomegalovirus (HCMV) infection and permissive for replication leading to release of infectious virions. Additionally, many basic ASC functions are inhibited during HCMV infection, such as differentiation and immunomodulatory potential. To our knowledge this is the first study examining potential adverse effects of HCMV infection on ASC biology. Our results suggest, that an active HCMV infection during ASC therapy may result in a poor clinical outcome due to interference by the virus.

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“…Indeed, the use of surface markers has been pivotal to recent progress in localizing ASCs within WAT. other studies ( 22,49,123,124 ). Pericytes thereby isolated using fl uorescence-activated cell sorting (FACS) were found to be multipotent, even after long-term passaging in culture, and to express MSC markers such as CD44, CD73, CD90, and CD105 ( Table 1 ) ( 121 ).…”

Section: Beauty Is Only Skin Deep: Cell Surface Markers In Asc Characmentioning

confidence: 97%

“…between different functional subgroups of ASCs, especially with regard to adipogenesis: although CD34-SVCs can undergo adipogenesis ( 47,121,123 ), CD34+ cells make up approximately 82% of the SVF ( 106 ) and are consistently more adipogenic in vitro than CD34-populations after negative selection for CD31 ( 124,161,189 ). Similarly, exclusion of CD34+ cells in muscle regeneration studies inhibits ectopic adipose formation both in vitro and in vivo ( 190 ), indicating that CD34-cells are incapable of adipogenesis.…”

Section: Ectodermal Cellsmentioning

confidence: 99%

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Cawthorn

Scheller

MacDougald

2012

Journal of Lipid Research

366

323

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with ill health. Such stigmatization distracts from a fascinating aspect of WAT biology: its enormous plasticity as an organ. Indeed, WAT is capable of massive expansion and contraction in response to chronic alterations in energy balance, accounting for as little as 5% body mass in extremely lean athletes ( 1 ) or as much as 60% body mass in morbidly obese individuals ( 2 ). Moreover in rodents, rabbits, and humans, WAT can regenerate following lipectomy ( 3-6 ). This striking degree of plasticity is unique among organs in adults. On a cellular level, WAT expansion is driven by both hypertrophy and hyperplasia of adipocytes ( 7-13 ). Even in nonexpanding WAT, adipocytes renew frequently to compensate for adipocyte death, with approximately 10% of adipocytes renewed annually ( 14,15 ). These data, which indicate that committed adipocyte progenitors (preadipocytes) exist within WAT, are the product of decades of research motivated largely by our desire to understand adipose tissue in the context of obesity and related diseases. Preadipocytes exist in adipose tissueThough the notion of obesity as a disease has existed since at least the time of Hippocrates ( 16 ), investigation into the origins of the adipocyte and the individual contributions of adipocyte hypertrophy and hyperplasia to adipose bulk began in earnest only after the appearance of the stem cell concept, as related to blood cells, in the early 1900s ( 17 ). In the 1940s, histologic observations of the appearance of adipocytes in chambers implanted in the ears of live rabbits suggested that de novo fat formation was Abstract White adipose tissue (WAT) is perhaps the most plastic organ in the body, capable of regeneration following surgical removal and massive expansion or contraction in response to altered energy balance. Research conducted for over 70 years has investigated adipose tissue plasticity on a cellular level, spurred on by the increasing burden that obesity and associated diseases are placing on public health globally. This work has identifi ed committed preadipocytes in the stromal vascular fraction of adipose tissue and led to our current understanding that adipogenesis is important not only for WAT expansion, but also for maintenance of adipocyte numbers under normal metabolic states. At the turn of the millenium, studies investigating preadipocyte differentiation collided with developments in stem cell research, leading to the discovery of multipotent stem cells within WAT. Such adipose tissue-derived stem cells (ASCs) are capable of differentiating into numerous cell types of both mesodermal and nonmesodermal origin, leading to their extensive investigation from a therapeutic and tissue engineering perspective. However, the insights gained through studying ASCs have also contributed to more-recent progress in attempts to better characterize committed preadipocytes in adipose tissue. Thus, ASC research has gone back to its roots, thereby expanding our knowledge of preadipocyte commitment and adipose tissue biology. -Cawthorn, W. P., E. ...

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Functional Implications of CD34 Expression in Human Adipose–Derived Stem/Progenitor Cells

Cited by 190 publications

References 39 publications

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

Adipocyte Regeneration after Free Fat Transplantation: Promotion by Stromal Vascular Fraction Cells

Human cytomegalovirus infection of human adipose-derived stromal/stem cells restricts differentiation along the adipogenic lineage

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

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