Functional heterogeneity of UDP-glucuronyltransferase in rat tissues

Bock, Karl Walter; Clausbruch, Ulrich C.V.; Kaufmann, R; Lilienblum, Werner; Oesch, Franz; Pfeil, H.; Platt, Karl L.

doi:10.1016/0006-2952(80)90368-8

Cited by 113 publications

(25 citation statements)

References 34 publications

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“…Our findings agree with the data of Emi et al (30) who found expression of the A2 RNA in spleen, lung, intestine, kidney, and testes, with undetectable levels in liver. Our results also are consistent with previous findings that 3-OH-BP and BPD UGT activities are detected in many extrahepatic tissues of rats (17). The molecular basis for the lower expression of UGT1A7 in rat liver is not clear.…”

Section: Figsupporting

confidence: 83%

“…In addition, while it is known that many UGT activities are inducible, few data exist regarding the regulation of BPD UGT. In attempting to classify BPD as a Group 1 (PAH-inducible) or Group 2 (phenobarbitalinducible) substrate, Bock et al (17) reported that BPD transferase activity was weakly inducible by either inducing agent and concluded that it was not clearly associated with either one of the two substrate groups.…”

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confidence: 99%

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Identification of a Rat Oltipraz-inducible UDP-glucuronosyltransferase (UGT1A7) with Activity Towards Benzo(a)pyrene-7,8-dihydrodiol

Grove

Kessler

Metz

et al. 1997

Journal of Biological Chemistry

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Previous work has shown that polycyclic aromatic hydrocarbons and oltipraz both induce an unidentified rat liver UDP-glucuronosyltransferase with activity toward benzo(a)pyrene-7,8-diol, the proximate carcinogenic form of benzo(a)pyrene. Here we report the isolation of a benzo(a)pyrene-7,8-diol transferase-encoding cDNA, LC14, from an adult rat hepatocyte-derived cell line (RALA255-10G LCS-3). The predicted amino acid sequence of LC14 is nearly identical (5 differences out of 531 residues) to that deduced from UGT1A7, recently cloned at the genomic DNA level (Emi, Y., Ikushiro, S., and Kyanagi, T. (

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Section: Figsupporting

confidence: 83%

mentioning

confidence: 99%

Identification of a Rat Oltipraz-inducible UDP-glucuronosyltransferase (UGT1A7) with Activity Towards Benzo(a)pyrene-7,8-dihydrodiol

Grove

Kessler

Metz

et al. 1997

Journal of Biological Chemistry

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“…The vasoconstrictor properties of 20-HETE (43), its major site of synthesis in the preglomerular microvasculature (28), and its release by AII and ET-1 (32,41), argue for its participation in the renal functional abnormalities associated with cirrhosis, particularly the initial reduction in RPF and the later depression of GFR. Conjugation of endogenous compounds with glucuronic acid, catalyzed by microsomal glucuronyl transferase, converts lipid-soluble hormones such as steroids into glucuronides, which can be more easily excreted (44). The glucuronide conjugate is usually biologically inactive (44), which appears to be the case for the 20-HETE glucuronide conjugate, as it was shown to be devoid of renal vasoactivity (M.A.…”

Section: Discussionmentioning

confidence: 99%

“…Conjugation of endogenous compounds with glucuronic acid, catalyzed by microsomal glucuronyl transferase, converts lipid-soluble hormones such as steroids into glucuronides, which can be more easily excreted (44). The glucuronide conjugate is usually biologically inactive (44), which appears to be the case for the 20-HETE glucuronide conjugate, as it was shown to be devoid of renal vasoactivity (M.A. Carroll, Department of Pharmacology, New York Medical College; personal communication).…”

Section: Discussionmentioning

confidence: 99%

Eicosanoid excretion in hepatic cirrhosis. Predominance of 20-HETE.

Sacerdoti¹,

Balazy²,

Angeli³

et al. 1997

J. Clin. Invest.

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The cytochrome P450 system transforms AA to hydroxyeicosatetraenoic acid (HETE) metabolites that are vasoactive and affect transport in several nephron segments. A principal product of this system, 20-HETE, participates in key mechanisms that regulate the renal circulation and extracellular fluid volume. We hypothesized that excess production of 20-HETE, which constricts the renal vasculature, contributes to the renal functional disturbances in patients with hepatic cirrhosis, particularly the depression of renal hemodynamics. The development of a precise and sensitive gas chromatographic/mass spectrometric method makes it possible to measure 20-HETE and the subterminal HETEs

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“…2A). UDPglucuronosyltransferase(1 -naphthol) is found almost ubiquitously in tissues of rats [29] and mice [21]. I t was therefore of interest to investigate whether enzymes from different rat tissues were immunochemically related.…”

Section: Resultsmentioning

confidence: 99%

Electroimmunochemical Quantification of UDP-glucuronosyltransferase in Rat Liver Microsomes

Pfeil

Bock

1983

Eur J Biochem

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Microsomal UDPglucuronosyltransferase(1 -naphthol), an enzyme form previously shown to be selectively inducible in rat liver by 3-methylcholanthrene-type inducers, was purified to apparent homogeneity. Rabbit antibodies against this enzyme form precipitated UDPglucuronosyltransferase activities towards 1 -naphthol and 4-methylumbelliferone faster and to greater extents than enzyme activities towards bilirubin, oestrone and 4-hydroxybiphenyl. Ouchterlony double-diffusion analysis showed immunochemical similarity of the rat liver enzyme with the enzymes from other organs of the rat (kidney, testes) and the mouse liver but not with the enzyme from cat and human liver. Electroimmunochemicd quantification of the enzyme indicated that its level was enhanced 1.3-fold and 2.5-fold in liver microsomes from phenobarbital-treated and 3-methylcholanthrene-treated rats, respectively. The results indicate that 3-methylcholanthrene treatment increases the enzyme level of rat liver microsomal UDPglucuronosyltransferase(3 -naphthol). Despite phospholipid-dependence of its catalytic activity microsomal enzyme activity appears to be a good index of the enzyme level.

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Functional heterogeneity of UDP-glucuronyltransferase in rat tissues

Cited by 113 publications

References 34 publications

Identification of a Rat Oltipraz-inducible UDP-glucuronosyltransferase (UGT1A7) with Activity Towards Benzo(a)pyrene-7,8-dihydrodiol

Identification of a Rat Oltipraz-inducible UDP-glucuronosyltransferase (UGT1A7) with Activity Towards Benzo(a)pyrene-7,8-dihydrodiol

Eicosanoid excretion in hepatic cirrhosis. Predominance of 20-HETE.

Electroimmunochemical Quantification of UDP-glucuronosyltransferase in Rat Liver Microsomes

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