Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus <i><scp>P</scp>lectosphaerella cucumerina</i> in <i><scp>A</scp>rabidopsis thaliana</i>

Ramos, Brisa; González‐Melendi, Pablo; Sánchez‐Vallet, Andrea; Sánchez‐Rodríguez, Clara; López, Gemma; Molina, Antonio

doi:10.1111/j.1364-3703.2012.00826.x

Cited by 28 publications

(31 citation statements)

References 91 publications

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“…These results were corroborated by confocal microscopy analyses of Col-0 and aba1-6 plants inoculated with PcBMM-GFP, a fungal transformant constitutively expressing GFP (Supplemental Fig. S1; Ramos et al, 2012). A positive correlation was found between in planta fungal biomass, plant cell death, and macroscopic disease symptoms (Fig.…”

supporting

confidence: 61%

Disruption of Abscisic Acid Signaling Constitutively Activates Arabidopsis Resistance to the Necrotrophic FungusPlectosphaerella cucumerina

et al. 2012

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Plant resistance to necrotrophic fungi is regulated by a complex set of signaling pathways that includes those mediated by the hormones salicylic acid (SA), ethylene (ET), jasmonic acid (JA), and abscisic acid (ABA). The role of ABA in plant resistance remains controversial, as positive and negative regulatory functions have been described depending on the plant-pathogen interaction analyzed. Here, we show that ABA signaling negatively regulates Arabidopsis (Arabidopsis thaliana) resistance to the necrotrophic fungus Plectosphaerella cucumerina. Arabidopsis plants impaired in ABA biosynthesis, such as the aba1-6 mutant, or in ABA signaling, like the quadruple pyr/pyl mutant (pyr1pyl1pyl2pyl4), were more resistant to P. cucumerina than wild-type plants. In contrast, the hab1-1abi1-2abi2-2 mutant impaired in three phosphatases that negatively regulate ABA signaling displayed an enhanced susceptibility phenotype to this fungus. Comparative transcriptomic analyses of aba1-6 and wild-type plants revealed that the ABA pathway negatively regulates defense genes, many of which are controlled by the SA, JA, or ET pathway. In line with these data, we found that aba1-6 resistance to P. cucumerina was partially compromised when the SA, JA, or ET pathway was disrupted in this mutant. Additionally, in the aba1-6 plants, some genes encoding cell wall-related proteins were misregulated. Fourier transform infrared spectroscopy and biochemical analyses of cell walls from aba1-6 and wild-type plants revealed significant differences in their Fourier transform infrared spectratypes and uronic acid and cellulose contents. All these data suggest that ABA signaling has a complex function in Arabidopsis basal resistance, negatively regulating SA/JA/ETmediated resistance to necrotrophic fungi.

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confidence: 61%

Disruption of Abscisic Acid Signaling Constitutively Activates Arabidopsis Resistance to the Necrotrophic FungusPlectosphaerella cucumerina

et al. 2012

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“…Loss of AGB1 confers even stronger phenotypes in many cases. agb1 null mutants are profoundly sensitive to many pathogens (18, 35, 56, 75, 83, 102, 105) and have short hypocotyls, altered leaf shape, and excess lateral roots and stomata (128). Both AtGPA1 and AGB1 operate in programmed cell death (55, 123).…”

Section: G Protein–mediated Sugar Signaling and Cellular Behaviorsmentioning

confidence: 99%

Heterotrimeric G Protein–Coupled Signaling in Plants

Urano¹,

Jones

2014

Annu. Rev. Plant Biol.

176

162

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Investigators studying G protein–coupled signaling—often called the best-understood pathway in the world owing to intense research in medical fields—have adopted plants as a new model to explore the plasticity and evolution of G signaling. Much research on plant G signaling has not disappointed. Although plant cells have most of the core elements found in animal G signaling, differences in network architecture and intrinsic properties of plant G protein elements make G signaling in plant cells distinct from the animal paradigm. In contrast to animal G proteins, plant G proteins are self-activating, and therefore regulation of G activation in plants occurs at the deactivation step. The self-activating property also means that plant G proteins do not need and therefore do not have typical animal G protein–coupled receptors. Targets of activated plant G proteins, also known as effectors, are unlike effectors in animal cells. The simpler repertoire of G signal elements in Arabidopsis makes G signaling easier to manipulate in a multicellular context.

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“…Effect of HWT on B. cinerea and P. expansum in vitro Spore germination needs to occur for a pathogen to establish infection of a plant host; thus, the ability to germinate is closely related to fungal pathogenicity (Nesher et al 2011;Ramos et al 2013). In the present study, HWT (45°C for 10 min) significantly reduced spore germination and inhibited germ tube elongation in both B. cinerea and P. expansum (Fig.…”

Section: Resultsmentioning

confidence: 99%

Ecofriendly hot water treatment reduces postharvest decay and elicits defense response in kiwifruit

Chen

Cheng

Wisniewski

et al. 2015

Environ Sci Pollut Res

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Hot water treatment (HWT) of fruit is an effective approach for managing postharvest decay of fruits and vegetables. In the present study, the effects of HWT (45 °C for 10 min) on the growth of Botrytis cinerea and Penicillium expansum in vitro, and gray (B. cinerea) and blue mold (P. expansum) development in kiwifruit were investigated. HWT effectively inhibited spore germination and germ tube elongation of B. cinerea and P. expansum. Reactive oxygen species accumulation and protein impairment in the fungi triggered by HWT contributed to the inhibitory effect. Results of in vivo studies showed that HWT controlled gray and blue mold in kiwifruit stored at 4 and 25 °C. HWT induced a significant increase in the activity of antioxidant enzymes, including catalase and peroxidase, and the level of total phenolic compounds in kiwifruit. These findings indicate that the inhibition of postharvest decay in kiwifruit by HWT is associated with the inhibition of spore germination of both fungal pathogens and the elicitation of defense response in the kiwifruit host. Moreover, HWT used in this study did not impair fruit quality. HWT appears to represent a potential non-chemical alternative for the effective management of postharvest decay of kiwifruit.

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Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana

Cited by 28 publications

References 91 publications

Disruption of Abscisic Acid Signaling Constitutively Activates Arabidopsis Resistance to the Necrotrophic FungusPlectosphaerella cucumerina

Disruption of Abscisic Acid Signaling Constitutively Activates Arabidopsis Resistance to the Necrotrophic FungusPlectosphaerella cucumerina

Heterotrimeric G Protein–Coupled Signaling in Plants

Ecofriendly hot water treatment reduces postharvest decay and elicits defense response in kiwifruit

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