Functional genomics: tools of the trade

Colebatch, Gillian; Trevaskis, Ben; Udvardi, Michael K.

doi:10.1046/j.0028-646x.2001.00303.x

Cited by 36 publications

(19 citation statements)

References 86 publications

(76 reference statements)

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“…Are these genes and their gene products produced during the early induction phase? Future improvements in gene detection and amplification procedures used with functional genomics (Colebatch et al, 2002) will enable us to identify additional proteins and genes that control the induction process. The ability to sort embryogenic from non-embryogenic cells using flow cytometric methods (Schulz andPauls, 1998, 2002) will provide us with a powerful tool in the study of the early events related to microspore induction.…”

Section: Conclusion and Perspeclivesmentioning

confidence: 99%

The canola microspore-derived embryo as a model system to study developmental processes in plants

Yeung

2002

J. Plant Biol.

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The ability of micrmpores to undergo embryo development after a successful induction treatment provides a unique experimental system to study a variety of developmental processes in plants. Recent published results focus on the cellular and molecular aspects of the early induction process. In this review, besides summarizing the current findings, the advantages of using the MDE system to study other aspects of embryo development are emphasized. The continual improvement of culturing procedures, media components, and molecular methods guarantees exciting new findings in the near future. Keywords: Brassica, embryo development, microspore-derived embryosThe reports by Maheshwari in 1964 and1966 demonstrated that microspores of a flowering plant, Datura, are capable of developing into haploid plants. This discovery clearly illustrates the added developmental potential of microspores as they can switch from gametophytic to sporophytic development under appropriate conditions. As a result, this observation has enabled further development of haploid plant technology. Since the original reports by Guha and Maheshwari, successes have been reported for different species (see Jain et al., 1996-t997). A majority of studies have been focused on tobacco, Brassica species, and different varieties of barley, rice and wheat. This is primarily due to their economic importance or their ease of manipulation. Although a large amount of information is available in the literature, emphasis is usually placed on the pre-culturing conditions, the stress treatments, the media components and the early cellular, biochemical and molecular events related to the induction process. The purpose of this review is to summarize recent findings and to draw attention to the usefulness of the rnicrospore-derived embryo (MDE) system in the study of other processes related to embryo development and maturation. Since not every aspect of MDE development will be discussed, readers are urged to consult reviews by Ferrie et al.

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Section: Conclusion and Perspeclivesmentioning

confidence: 99%

The canola microspore-derived embryo as a model system to study developmental processes in plants

Yeung

2002

J. Plant Biol.

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“…Complex trade‐offs require the integration of many different processes related to, for example, carbon and nitrogen metabolism, water use and stress tolerance. The new tools offered by functional genomics methodology (Colebatch et al ., 2002) enable also the ‘horizontal’ analysis of hundreds of genes, transcripts, proteins and metabolites at a time, and therefore have a great potential to complement the traditional methods with increased power for the investigation of even complex trade‐offs. For example, Seki et al .…”

Section: Need For Integrated Approachesmentioning

confidence: 99%

Trade‐offs in plants and the prospects for breeding using modern biotechnology

Weih

2003

New Phytologist

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“…Furthermore, tools for analysis of the transcriptome, proteome and metabolome have also attained an unprecedented level of sophistication. These tools have been referred to as being part of the functional genomics toolbox (Colebatch et al 2002). Although the gamut of implements may be perceived as being task-specific, in effect they complement one another at one point or the other, resulting in a more refined understanding of the genome and identification of its functional attributes, leading to the application of functional genomics tools to plant breeding practices ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Functional Genomics For Crop Improvement

Ganeshan

Sharma

Chibbar

2009

Molecular Techniques in Crop Improvement

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Plant breeding has had a tremendous influence on crop improvement. However, due to dwindling germplasm resources, identification of variability for incorporation into new cultivars is becoming more difficult. Therefore, there has been recourse to alternative approaches including mutagenesis, tissue culture and genetic transformation to aid breeding programs. Furthermore, with the vast repertoire of genome-wide data from different expression profiling techniques such as microarrays, more subtle understanding of gene expression is being obtained and is further helping plant breeders to entertain a different selection approach based on expression quantitative traits to maximize combinations of genes capable of conferring high performance. In this chapter, we review some of the aspects of plant breeding and the influence functional genomics has on breeding programs. Some of the challenges to functional genomics and breeding come from establishment of high-throughput transformation systems to assess gene function, which is limiting functional characterization of numerous genes in their respective crops. Therefore, this chapter also focuses on the need to gain better understanding of the development of gene transfer systems for crop plants to make use of the array of available gene information data.

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Functional genomics: tools of the trade

Cited by 36 publications

References 86 publications

The canola microspore-derived embryo as a model system to study developmental processes in plants

The canola microspore-derived embryo as a model system to study developmental processes in plants

Trade‐offs in plants and the prospects for breeding using modern biotechnology

Functional Genomics For Crop Improvement

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