Functional genomics to understand the tolerance mechanism against biotic and abiotic stresses in Capsicum species

Vijay, Archa; Kumar, Ajay; Islam, Khushbu; Momo, John; Ramchiary, Nirala

doi:10.1016/b978-0-323-91810-7.00001-7

Cited by 4 publications

(2 citation statements)

References 176 publications

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“…However, the growth and yield of chilli pepper are severely impeded by multiple environmental stresses, including drought, heat, salinity, and a broad spectrum of pathogens, including fungi, bacteria, viruses, nematodes, and insects (Vijay et al, 2023). Drought is particularly detrimental, notably when it occurs during the flowering stage, leading to reduced fruit setting and diminished capsaicin content in peppers (Kopta et al, 2020, Anjum et al, 2012, Naresh et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Enhancing drought tolerance in chilli pepper through AdDjSKI‐mediated modulation of ABA sensitivity, photosynthetic preservation, and ROS scavenging

Bulle,

Venkatapuram,

Rahman

et al. 2024

Physiologia Plantarum

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Drought stress threatens the productivity of numerous crops, including chilli pepper (Capsicum annuum). DnaJ proteins are known to play a protective role against a wide range of abiotic stresses. This study investigates the regulatory mechanism of the chloroplast‐targeted chaperone protein AdDjSKI, derived from wild peanut (Arachis diogoi), in enhancing drought tolerance in chilli peppers. Overexpressing AdDjSKI in chilli plants increased chlorophyll content, reflected in the maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm) compared with untransformed control (UC) plants. This enhancement coincided with the upregulated expression of PSII‐related genes. Our subsequent investigations revealed that transgenic chilli pepper plants expressing AdDjSKI showed reduced accumulation of superoxide and hydrogen peroxide and, consequently, lower malondialdehyde levels and decreased relative electrolyte leakage percentage compared with UC plants. The mitigation of ROS‐mediated oxidative damage was facilitated by heightened activities of antioxidant enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase, coinciding with the upregulation of the expression of associated antioxidant genes. Additionally, our observations revealed that the ectopic expression of the AdDjSKI protein in chilli pepper plants resulted in diminished ABA sensitivity, consequently promoting seed germination in comparison with UC plants under different concentrations of ABA. All of these collectively contributed to enhancing drought tolerance in transgenic chilli plants with improved root systems when compared with UC plants. Overall, our study highlights AdDjSKI as a promising biotechnological solution for enhancing drought tolerance in chilli peppers, addressing the growing global demand for this economically valuable crop.

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Section: Introductionmentioning

confidence: 99%

Enhancing drought tolerance in chilli pepper through AdDjSKI‐mediated modulation of ABA sensitivity, photosynthetic preservation, and ROS scavenging

Bulle,

Venkatapuram,

Rahman

et al. 2024

Physiologia Plantarum

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“…From these significant traits, genetic lines with resistances to diverse pathogens, such as viruses, bacteria, and fungi, are of ecological and economical relevance during plant and seed development stages [7][8][9][10]. In addition to modern breeding programs, recently, diverse methodologies, such as metagenomics, transcriptomics, proteomics, or metabolomics approaches, have been relevant to understanding microorganism-plant interactions or plant development during the lifecycle of Capsicum [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity

Hernández Flores,

Martínez,

Ramos López

et al. 2023

Microorganisms

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The Kosakonia cowanii Cp1 strain was isolated from seeds of Capsicum pubescens R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity test revealed nonpathogenic traits confirmed by the absence of specific virulence-related genes. Interestingly, when fungal inhibitory essays were carried out, the bacterial synthesis of volatile organic compounds (VOCs) with antifungal activity showed that Sclerotinia sp. and Rhizoctonia solani were inhibited by 87.45% and 77.24%, respectively. Meanwhile, Sclerotium rolfsii, Alternaria alternata, and Colletotrichum gloeosporioides demonstrated a mean radial growth inhibition of 52.79%, 40.82%, and 55.40%, respectively. The lowest inhibition was by Fusarium oxysporum, with 10.64%. The VOCs’ characterization by headspace solid–phase microextraction combined with gas chromatography–mass spectrometry (HS-SPME-GC–MS) revealed 65 potential compounds. Some of the compounds identified with high relative abundance were ketones (22.47%), represented by 2-butanone, 3-hydroxy (13.52%), and alcohols (23.5%), represented by ethanol (5.56%) and 1-butanol-3-methyl (4.83%). Our findings revealed, for the first time, that K. cowanii Cp1 associated with C. pubescens seeds possesses potential traits indicating that it could serve as an effective biocontrol.

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Molecular and biotechnological approaches for improvement of pepper crop

Islam,

Momo,

Ramchiary

2024

Pepper Virome

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Functional genomics to understand the tolerance mechanism against biotic and abiotic stresses in Capsicum species

Cited by 4 publications

References 176 publications

Enhancing drought tolerance in chilli pepper through AdDjSKI‐mediated modulation of ABA sensitivity, photosynthetic preservation, and ROS scavenging

Enhancing drought tolerance in chilli pepper through AdDjSKI‐mediated modulation of ABA sensitivity, photosynthetic preservation, and ROS scavenging

Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 Isolated from the Seeds of Capsicum pubescens R & P Possess Antifungal Activity

Molecular and biotechnological approaches for improvement of pepper crop

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