Functional genomics of hsp-90 in parasitic and free-living nematodes

Gillan, Victoria; Maitland, Kirsty; McCormack, Gillian; Him, Nik Ahmad Irwan Izzauddin Nik; Devaney, Eileen

doi:10.1016/j.ijpara.2009.02.024

Cited by 38 publications

(38 citation statements)

References 49 publications

(38 reference statements)

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“…In order to confirm the involvement of DAF-21 in the observed phenotypes, we reduced DAF-21 levels in wt worms by feeding RNA interference (RNAi) using a construct described before [14], [28]. Worms remained sterile as previously observed [13]–[15]. We used worms at day 4 and day 7 after hatching and measured the frequency of thrashing (Table 1).…”

Section: Resultsmentioning

confidence: 99%

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Downregulation of the Hsp90 System Causes Defects in Muscle Cells of Caenorhabditis Elegans

et al. 2011

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The ATP-dependent molecular chaperone Hsp90 is required for the activation of a variety of client proteins involved in various cellular processes. Despite the abundance of known client proteins, functions of Hsp90 in the organismal context are not fully explored. In Caenorhabditis elegans, Hsp90 (DAF-21) has been implicated in the regulation of the stress-resistant dauer state, in chemosensing and in gonad formation. In a C. elegans strain carrying a DAF-21 mutation with a lower ATP turnover, we observed motility defects. Similarly, a reduction of DAF-21 levels in wild type nematodes leads to reduced motility and induction of the muscular stress response. Furthermore, aggregates of the myosin MYO-3 are visible in muscle cells, if DAF-21 is depleted, implying a role of Hsp90 in the maintenance of muscle cell functionality. Similar defects can also be observed upon knockdown of the Hsp90-cochaperone UNC-45. In life nematodes YFP-DAF-21 localizes to the I-band and the M-line of the muscular ultrastructure, but the protein is not stably attached there. The Hsp90-cofactor UNC-45-CFP contrarily can be found in all bands of the nematode muscle ultrastructure and stably associates with the UNC-54 containing A-band. Thus, despite the physical interaction between DAF-21 and UNC-45, apparently the two proteins are not always localized to the same muscular structures. While UNC-45 can stably bind to myofilaments in the muscular ultrastructure, Hsp90 (DAF-21) appears to participate in the maintenance of muscle structures as a transiently associated diffusible factor.

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Section: Resultsmentioning

confidence: 99%

“…Daf-21 is an essential gene, as observed by RNAi and knockout strains [15]. Given the essential nature of DAF-21, we were intrigued by the mild phenotype of the daf-21(p673) nematodes.…”

Section: Resultsmentioning

confidence: 99%

Downregulation of the Hsp90 System Causes Defects in Muscle Cells of Caenorhabditis Elegans

et al. 2011

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“…These pathways have also been implicated in development, metabolism and behaviour in other phylogenetically distant free-living nematodes, APNs (Ogawa et al , 2009; Bento et al , 2010; Albarqi et al , 2016) and in the PPN B. xylophilus (Zhao et al , 2013). Other genes from the DAF pathway have been reported to be necessary for parasitism, such as DAF-21 / HSP90 (Birnby et al , 2000; Gillan et al , 2009; Lourenço-Tessutti et al , 2015). This finding raises the question of whether other DAF genes are determinants at different steps during the parasitic nematode life cycle.…”

Section: Section Ii: Potential Key Regulators Of Oxidative Stress Resmentioning

confidence: 99%

Plant-parasitic nematodes: towards understanding molecular players in stress responses

Gillet

Bournaud

Júnior

et al. 2017

Ann Bot

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Background Plant–parasitic nematode interactions occur within a vast molecular plant immunity network. Following initial contact with the host plant roots, plant-parasitic nematodes (PPNs) activate basal immune responses. Defence priming involves the release in the apoplast of toxic molecules derived from reactive species or secondary metabolism. In turn, PPNs must overcome the poisonous and stressful environment at the plant–nematode interface. The ability of PPNs to escape this first line of plant immunity is crucial and will determine its virulence. Scope Nematodes trigger crucial regulatory cytoprotective mechanisms, including antioxidant and detoxification pathways. Knowledge of the upstream regulatory components that contribute to both of these pathways in PPNs remains elusive. In this review, we discuss how PPNs probably orchestrate cytoprotection to resist plant immune responses, postulating that it may be derived from ancient molecular mechanisms. The review focuses on two transcription factors, DAF-16 and SKN-1, which are conserved in the animal kingdom and are central regulators of cell homeostasis and immune function. Both regulate the unfolding protein response and the antioxidant and detoxification pathways. DAF-16 and SKN-1 target a broad spectrum of Caenorhabditis elegans genes coding for numerous protein families present in the secretome of PPNs. Moreover, some regulatory elements of DAF-16 and SKN-1 from C. elegans have already been identified as important genes for PPN infection. Conclusion DAF-16 and SKN-1 genes may play a pivotal role in PPNs during parasitism. In the context of their hub status and mode of regulation, we suggest alternative strategies for control of PPNs through RNAi approaches.

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“…The greater efficiency of complementation by Hc-daf-16.2 may reflect the closer phylogenetic relationship between C. elegans and H. contortus (both within clade V), compared with the relatively distant relationship of C. elegans and S. stercoralis (a member of clade IV (Blaxter et al, 1998). A similar correlation between cross-species complementation efficiency and phylogenetic relatedness is seen in the case of parasite orthologues of the heat shock protein 90 (HSP-90) encoded by C. elegans daf-21 (Gillan et al, 2009). These authors found that the H. contortus orthologue Hc-hsp-90 can partially complement a daf-21 mutation in C. elegans , whereas the orthologue Bm-hsp-90 from the filarial nematode, B. malayi (a member of nematode clade III (Blaxter et al, 1998) cannot.…”

Section: Discussionmentioning

confidence: 56%

Structural and functional characterisation of the fork head transcription factor-encoding gene, Hc-daf-16, from the parasitic nematode Haemonchus contortus (Strongylida)

Hu¹,

Lok²,

Ranjit³

et al. 2010

International Journal for Parasitology

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Despite their phylogenetic diversity, parasitic nematodes share attributes of longevity and developmental arrest (=hypobiosis) with free-living nematodes at key points in their life cycles, particularly in larval stages responsible for establishing infection in the host. Insulin-like signalling plays crucial roles in the regulation of life span and arrest (=dauer formation) in the free-living nematode, Caenorhabditis elegans. Insulin-like signalling in C. elegans negatively regulates the fork head boxO (FoxO) transcription factor encoded by daf-16, which is linked to initiating a dauerspecific pattern of gene expression. Orthologues of daf-16 have been identified in several species of parasitic nematode. Although function has been demonstrated for an orthologue from the parasitic nematode Strongyloides stercoralis (Rhabditida), the functional capabilities of homologues/ orthologues in bursate nematodes (Strongylida) are unknown. In the present study, we used a genomic approach to determine the structures of two complete daf-16 orthologues and their transcripts in the parasitic nematode Haemonchus contortus, and assessed their function(s) using C. elegans as a genetic surrogate. Unlike the multiple isoforms of Ce- which

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Functional genomics of hsp-90 in parasitic and free-living nematodes

Cited by 38 publications

References 49 publications

Downregulation of the Hsp90 System Causes Defects in Muscle Cells of Caenorhabditis Elegans

Downregulation of the Hsp90 System Causes Defects in Muscle Cells of Caenorhabditis Elegans

Plant-parasitic nematodes: towards understanding molecular players in stress responses

Structural and functional characterisation of the fork head transcription factor-encoding gene, Hc-daf-16, from the parasitic nematode Haemonchus contortus (Strongylida)

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