Functional genomics in farm animals – Microarray analysis

Berthou, Christian; Hedegaard, Jakob; Horn, Per

doi:10.1016/j.meatsci.2005.04.011

Cited by 10 publications

(1 citation statement)

References 72 publications

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“…After hybridization of the array-labelled RNA, the chip is scanned and the fluorescent signal at each point on the chip with a cDNA probe provides a measure of the expression of the corresponding gene. Their functioning basically consists of measuring the level of hybridization between the specific probe, and the target molecule, and they are generally indicated by fluorescence and through a computer analysis of the image, which indicates the level of expression of the corresponding gene (Bendixen et al 2005). The numerous studies developed with microarrays in sheep provided a large amount of information at a functional level in relation to metabolic processes (Satterfield et al 2009;Chang et al 2019), nematode resistance (Diez-Tascón et al 2005;Keane et al 2006;, immune responses (Galindo et al 2008) and skin pigmentation (García-Gámez et al 2011;Peñagaricano et al 2012) among others.…”

Section: Functional Genomics In Sheep Breedingmentioning

confidence: 99%

Use of genomic tools for the study of the genetic basis of resistance to gastrointestinal nematode infections in adult sheep = Utilización de herramientas genómicas para el estudio de la base genética de la resistencia a las infecciones por nematodos gastrointestinales en ovejas adultas

Chitneedi¹

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Considering all this, the present Thesis was planned with the global objective of using genomic tools for the study of the genetic basis of resistance to gastrointestinal nematode infections in adult sheep.To reach this global objective in an efficient way, and following the established official research plan for this research project, two specific objectives have been defined: 1. To refine and high-resolution mapping of QTL influencing GIN resistance in adult sheep. Imputation of SNP-chip genotypes and whole genome sequencing will be exploited to identify QTL and potential causal mutations influencing indicator traits of GIN resistance traits in a commercial population of dairy sheep exposed to GIN natural infection. Also, HD-chip imputed genotypes will be used to update LD estimations and other population parameters (Ne, F, ROH extent).2. Perform a global transcriptomic study of target abomasal tissues after an experimental challenge with Teladorsagia circumcincta in adult ewes previously classified as resistant and susceptible. RNA-seq datasets will be analysed to identify differential transcript expression patterns of genes and long non-coding RNAs (lnc-RNA) and also to identify potential causal mutations related to GIN resistance.Praveen Krishna Chitneedi -PhD Thesis traits, including udder and body conformation, and number of born lambs were later considered in the year 2006 with the collection of the first lambs as future progeny test sires. Impact of GIN infection in sheep and control strategiesGIN infection is one of the major health related issues faced by the sheep farming industry worldwide, which incurs major economic losses for the farmers in the form of the cost associated with anthelmintic treatment and the negative effect in the overall productivity, fertility and survival of sheep with a range of health issues (Dominik, 2005;Mavrot et al. 2015). The prevalence of nematode infection is mostly dependent on the optimal conditions for the development of nematode species in the sheep rearing regions (O'Connor et al. 2006) and also requires sufficient humidity to facilitates the free-living stages of the nematode life cycle (Martínez-Valladares et al. 2013b). The seasonal changes also effect the prevalence of nematodes in a given region. The Castilla y León region, with has a Mediterranean climate with continental influences, is characterised by long cold winters and warm summers, which favours the prevalence of GIN nematodes like Teladorsagia circumcincta (Martínez-Valladares et al. 2013b). Moreover, the prevalence of GIN infections in this region was previously reported and found to play a significant role in the overall well-being of the sheep industry of Castilla y León (Martínez-González et al. 1998;Martínez-Valladares et al. 2013b). Classically, the control of GIN infections in sheep populations used to be carried out using anthelmintic drugs, but GIN resistance against anthelmintic drugs gradually appeared and in sheep flocks, including those included in the region of Castilla y León (Martínez-...

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