Functional Genomics and Bioinformatics of the Phytophthora sojae Soybean Interaction

Tyler, Brett M.; Jiang, Rays H. Y.; Zhou, Lecong; Tripathy, Sucheta; Dou, Deqiang; Torto-Alalibo, Trudy; Li, Hua; Mao, Yongcai; Liu, Bing; Vega‐Sánchez, Miguel E.; Mideros, Santiago X.; Hanlon, Regina; Smith, Brian M.; Krampis, Konstantinos; Ye, Keying; Martin, Steven St.; Dorrance, Anne E.; Hoeschele, Ina; Maroof, M. A. Saghai

doi:10.1007/978-0-387-76723-9_6

Cited by 9 publications

(12 citation statements)

References 51 publications

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“…It is durable against all races of P . sojae and highly heritable [ 8 , 9 ]. Therefore, partial resistance provides an effective way to develop Phytophthora resistant cultivars.…”

Section: Introductionmentioning

confidence: 99%

“…The amount of preformed suberin was also found contribute to the partial resistance [ 21 , 22 ]. Using soybean Aymetrix gene chips, whole-genome transcription profiles were analyzed in soybean genotypes with differential levels of partial resistance [ 8 , 13 , 23 ]. Appraising the differential transcript genes in Conrad and Sloan underlying the QTL found that most of them encompassed putative physiological trait genes, defense-related genes, and disease resistance-like genes [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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GmCYP82A3, a Soybean Cytochrome P450 Family Gene Involved in the Jasmonic Acid and Ethylene Signaling Pathway, Enhances Plant Resistance to Biotic and Abiotic Stresses

et al. 2016

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The cytochrome P450 monooxygenases (P450s) represent a large and important enzyme superfamily in plants. They catalyze numerous monooxygenation/hydroxylation reactions in biochemical pathways, P450s are involved in a variety of metabolic pathways and participate in the homeostasis of phytohormones. The CYP82 family genes specifically reside in dicots and are usually induced by distinct environmental stresses. However, their functions are largely unknown, especially in soybean (Glycine max L.). Here, we report the function of GmCYP82A3, a gene from soybean CYP82 family. Its expression was induced by Phytophthora sojae infection, salinity and drought stresses, and treatment with methyl jasmonate (MeJA) or ethephon (ETH). Its expression levels were consistently high in resistant cultivars. Transgenic Nicotiana benthamiana plants overexpressing GmCYP82A3 exhibited strong resistance to Botrytis cinerea and Phytophthora parasitica, and enhanced tolerance to salinity and drought stresses. Furthermore, transgenic plants were less sensitive to jasmonic acid (JA), and the enhanced resistance was accompanied with increased expression of the JA/ET signaling pathway-related genes.

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“…It is durable against all races of P . sojae and highly heritable [ 8 , 9 ]. Therefore, partial resistance provides an effective way to develop Phytophthora resistant cultivars.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GmCYP82A3, a Soybean Cytochrome P450 Family Gene Involved in the Jasmonic Acid and Ethylene Signaling Pathway, Enhances Plant Resistance to Biotic and Abiotic Stresses

et al. 2016

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“…Several studies have used the soybean Affymetrix gene chips to profile the infection response to pathogens such as Heterodera glycines Ichinohe (Ithal et al, 2007; Klink et al, 2009), Phakopsora pachyrhizi Sydow (Panthee et al, 2007; van de Mortel et al, 2007), and P. sojae (Tyler et al, 2007; Waller et al, 2007; Zhou et al, 2009). Each chip contains ∼37,500 soybean transcripts, ∼15,800 transcripts for P. sojae , as well as 7500 transcripts from H. glycines .…”

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confidence: 99%

“…Changes in transcript abundance in soybean cultivars with different levels of partial resistance to P. sojae were examined in two previous microarray experiments (“eight cultivar” and “four cultivar;” Tyler et al, 2007; Waller et al, 2007; Zhou et al, 2009). In the eight cultivar experiment, eight soybean genotypes were sampled at 3 and 5 DAI, Conrad, ‘Athow’, V71‐370, and ‘General’ with high levels of partial resistance; ‘Williams’ and PI291327 with moderate levels of partial resistance; ‘Sloan’ with a low level of partial resistance; and OX20‐8 which is highly susceptible to P. sojae (Tyler et al, 2007). In the four cultivar experiment, Conrad, V71‐370, Sloan, and the susceptible recombinant inbred line, VP‐RIL9, from the cross of V71‐370 by PI 407162, were examined for changes in transcript abundance at 1, 2, 3, and 5 DAI (Waller et al, 2007).…”

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confidence: 99%

“…In the four cultivar experiment, Conrad, V71‐370, Sloan, and the susceptible recombinant inbred line, VP‐RIL9, from the cross of V71‐370 by PI 407162, were examined for changes in transcript abundance at 1, 2, 3, and 5 DAI (Waller et al, 2007). In the eight cultivar experiment, ∼25,000 genes had statistically significant responses to infection, with little difference between Day 3 and Day 5 samplings (Tyler et al, 2007). At 5 DAI, 97∼99% of the detectable genes had moderate transcription changes (mostly less than two‐fold) in response to infection and soybean genotype, and these low‐magnitude changes were consistent across biological replicates (Zhou et al, 2009).…”

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Analysis of Genes Underlying Soybean Quantitative Trait Loci Conferring Partial Resistance to Phytophthora sojae

et al. 2010

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Few quantitative trait loci (QTL) have been mapped for the expression of partial resistance to Phytophthora sojae in soybean and very little is known about the molecular mechanisms that contribute to this trait. Therefore, the objectives of this study were to identify additional QTL conferring resistance to P. sojae and to identify candidate genes that may contribute to this form of defense. QTL on chromosomes 12, 13, 14, 17, and 19, each explaining 4 to 7% of the phenotypic variation, were identifi ed using 186 RILs from a cross of the partially resistant cultivar 'Conrad' and susceptible cultivar 'Sloan' through composite interval mapping. Microarray analysis identifi ed genes with signifi cant differences in transcript abundances between Conrad and Sloan, both constitutively and following inoculation. Of these genes, 55 mapped to the fi ve QTL regions. Ten genes encoded proteins with unknown functions, while the others encode proteins related to defense or physiological traits. Seventeen genes within the genomic region that encompass the QTL were selected and their transcript abundance was confi rmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These results suggest a complex QTL-mediated resistance network. This study will contribute to soybean resistance breeding by providing additional QTL for marker-assisted selection as well as a list of candidate genes which may be manipulated to confer resistance.

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Plants and oomycetes, an intimate relationship: co-evolutionary principles and impact on agricultural practice.

et al. 2010

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Plants face continuous attacks from a broad range of pathogens and have evolved effective defence mechanisms that are initiated upon pathogen attack. Invading oomycete pathogens secrete effectors, molecules that manipulate host cell defence and thereby enable colonization. However, plant species evolved resistance (R) genes to most specialized pathogen species. The R proteins can detect effectors, termed avirulence (AVR) proteins, and thus confer immunity to pathogens. Effectors and their interacting genes in the plant play a central role in the co-evolution of pathogens with their hosts. In this review, we discuss the role that effectors play in the pathogenesis and lifestyles of oomycetes. Particularly intriguing features emerge for (hemi-)biotrophic oomycetes, which establish an intimate contact with the host by forming haustoria. At this interface, effectors with an RXLR motif are translocated into the cytoplasm, where they reprogramme the host towards susceptibility. Such interactions between effectors and host targets are highly specific and are considered a result of tight co-evolution. In addition, we elaborate on the Phytophthora infestans-Solanum pathosystem, from which various R and Avr genes were cloned recently. We discuss a rationale for exploiting molecular insights into R-Avr interactions for developing more durable resistance strategies to control late blight in agriculture.

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Functional Genomics and Bioinformatics of the Phytophthora sojae Soybean Interaction

Cited by 9 publications

References 51 publications

GmCYP82A3, a Soybean Cytochrome P450 Family Gene Involved in the Jasmonic Acid and Ethylene Signaling Pathway, Enhances Plant Resistance to Biotic and Abiotic Stresses

GmCYP82A3, a Soybean Cytochrome P450 Family Gene Involved in the Jasmonic Acid and Ethylene Signaling Pathway, Enhances Plant Resistance to Biotic and Abiotic Stresses

Analysis of Genes Underlying Soybean Quantitative Trait Loci Conferring Partial Resistance to Phytophthora sojae

Plants and oomycetes, an intimate relationship: co-evolutionary principles and impact on agricultural practice.

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