Functional Genomics Analysis of Singapore Grouper Iridovirus: Complete Sequence Determination and Proteomic Analysis

iTRAQ analysis of Singapore grouper iridovirus infection in a grouper embryonic cell line

“…Some of their encoded ORFs, such as ORF018R, ORF026R and ORF093L, were previously reported as full-length putative genes (Song et al, 2004). In this study, we were able to show using Western blot with specific antibodies that these ORFs encode full-length protein products (Fig.…”

“…Only ORF022L, which has been reported by Song et al (2006), was identified at 86.351 CI %. Of these 49 proteins, 11 were discovered for the first time (Table 1), the remaining 38 proteins (Table 2) have been reported previously (Song et al, 2004. In addition, 10 of the 11 newly identified proteins, except ORF049L that is predicted to be a dUTPase-like protein (Song et al, 2004), are novel proteins with unknown functions.…”

“…Recently, Chen and coworkers discovered that 127 ORFs of SGIV were transcriptionally active (Chen et al, 2006). Although a total of 51 SGIV proteins have been identified, the translational products of the remaining 111 ORFs are unknown (Song et al, 2004. All these earlier studies focused on the identification and structural characterization of the viral proteins.…”

“…SGIV was isolated from brown-spotted grouper in 1998 (Qin et al, 2001) and its genome was successfully sequenced with 162 predicted open reading frames (ORFs). Twenty-six SGIV proteins were later identified by proteomics analysis (Song et al, 2004). An additional 25 SGIV viral proteins were reported in 2006 .…”

Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein

“…Based on the Eighth Report of the International Committee on Taxonomy of Virus (ICTV), the family Iridoviridae has been subdivided into five genera: Iridovirus, Chloriridovirus, Ranavirus, Lymphocystisvirus, and Megalocytivirus [3]. To date, 12 genome sequences of iridoviruses including at least one from each genus have been completely sequenced and analysed [2,[4][5][6][7][8][9][10][11][12][13][14]. These analyses show that iridoviruses encode a number of cellular protein homologues that may be mostly involved in nucleic acid metabolism, such as ribonucleotide reductase (RR), thymidylate synthase (TS), deoxyuridine triphosphatase (DUT), and purine nucleoside phosphorylase (PNP) [15].…”