Functional genomic characterization unveils probiotic features of   G1-11 isolated from the gut of the hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂)

Xie, Gary; Zhu, Yating; Zhong, Zhongxuan; Du, Qianping; Wu, Yuxin; Xu, Ke; Zhang, Mingqing; Shu, Hu

doi:10.1016/j.lwt.2023.115088

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…The results are a reflection of the recently conducted study by Tiwari et al (2024) where the bacterial cell's hydrophobicity for the attachment with PE, and nylon MPs was investigated to validate the biofilm formation and degradation. The hydrophobicity of the same bacterial species showed more than 90% hydrophobicity for xylene, and hexane hydrocarbons which is noticeably higher than the SHBF2 strain of the present study (Xie et al, 2023). The reason for this contrasting behavior from the same species has been answered by the study of Fadila, (2016) where they got 12 grouped strains of B. cereus that had different hydrophobicity for xylene, hexane, ether, and chloroform hydrocarbons.…”

Section: Cell Surface Hydrophobicity Of B Cereus Shbf2contrasting

confidence: 46%

Biodegradation of polyethylene (PE), polypropylene (PP), and polystyrene (PS) microplastics by floc-forming bacteria, Bacillus cereus strain SHBF2, isolated from a commercial aquafarm

Hossain,

Shukri,

Waiho

et al. 2024

Environ Sci Pollut Res

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The ubiquitous proximity of the commonly used microplastic (MP) particles particularly polyethylene (PE), polypropylene (PP), and polystyrene (PS) poses a serious threat to the environment, and human health globally.Biological treatment as an environment-friendly approach to counter MPs pollution has recent interest when the bioagent has beneficial functions in their ecosystem. This study aimed to utilize beneficial floc-forming bacteria B. cereus SHBF2 isolated from an aquaculture farm in reducing the MPs particles (PE, PP, and PS) from their environment. The bacteria were inoculated for 60 days in a media containing MPs particles as a sole carbon source. On different days of incubation (DOI), the bacterial growth analysis was monitored and the MPs particles were harvested to examine their weight loss, surface changes, and alterations in chemical properties. After 60 DOI, the highest weight loss was recorded for PE, 6.87 ± 0.92%, which was further evaluated to daily reduction rate (k), 0.00118 gday -1 , and half-life (t 1/2 ), 605.08 ± 138.52 days. The OD value (1.74 ± 0.008 Abs.) indicated the higher efficiency of bacteria for PP utilization, and so for the colony formation per define volume (1.04 × 10 11 CFU/mL). Biofilm formation, erosions, cracks, and fragments were evident during the observation of the tested MPs using the scanning electron microscope (SEM). The formation of carbonyl and alcohol group due to the oxidation and hydrolysis by SHBF2 strain were confirmed using the Fourier transform infrared spectroscopic (FTIR) analysis. Additionally, the alterations of pH and CO 2 evolution from each of the MPs type ensures the bacterial activity and mineralization of the MPs particles. The findings of this study have confirmed and indicated a higher degree of biodegradation for all of the selected MPs particles. B. cereus SHBF2, the floc-forming bacteria used in aquaculture, has demonstrated a great potential for use as an efficient MPs degrading bacterium in the biofloc farming system in the near future to guarantee a sustainable green aquaculture production.

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Section: Cell Surface Hydrophobicity Of B Cereus Shbf2contrasting

confidence: 46%

Biodegradation of polyethylene (PE), polypropylene (PP), and polystyrene (PS) microplastics by floc-forming bacteria, Bacillus cereus strain SHBF2, isolated from a commercial aquafarm

Hossain,

Shukri,

Waiho

et al. 2024

Environ Sci Pollut Res

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“…paracasei and Lp. plantarum strains the presence of genes encoding classic heat-shock proteins such as GrpE and DnaK [52], pyruvate kinases involved in acid resistance [53], and choloylglycine hydrolase and CTP synthase, which confer resistance to bile salts [54,55]. Moreover, several osmoprotectant uptake systems that belong to the opu family were detected in the four probiotic candidates.…”

Section: Probiotic Traitsmentioning

confidence: 99%

“…These uptake systems grant the candidate strains tools to tolerate severe osmotic stress and could also be involved in enduring low-and high-temperature conditions [56]. Lastly, several genes involved in oxidative stress tolerance were predicted, namely, glutathione, pyruvate, and thiol peroxidases [11,55].…”

Section: Probiotic Traitsmentioning

confidence: 99%

Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture

Contente,

Díaz-Formoso,

Feito

et al. 2024

Genes

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Aquaculture plays a crucial role in meeting the increasing global demand for food and protein sources. However, its expansion is followed by increasing challenges, such as infectious disease outbreaks and antibiotic misuse. The present study focuses on the genetic and functional analyses of two Lacticaseibacillus paracasei (BF3 and RT4) and two Lactiplantibacillus plantarum (BF12 and WT12) strains isolated from a rotifer cultivation tank used for turbot larviculture. Whole-genome sequencing (WGS) and bioinformatics analyses confirmed their probiotic potential, the absence of transferable antibiotic resistance genes, and the absence of virulence and pathogenicity factors. Bacteriocin mining identified a gene cluster encoding six plantaricins, suggesting their role in the antimicrobial activity exerted by these strains. In vitro cell-free protein synthesis (IV-CFPS) analyses was used to evaluate the expression of the plantaricin genes. The in vitro-synthesized class IIb (two-peptide bacteriocins) plantaricin E/F (PlnE/F) exerted antimicrobial activity against three indicator microorganisms, including the well-known ichthyopathogen Lactococcus garvieae. Furthermore, MALDI-TOF MS on colonies detected the presence of a major peptide that matches the dimeric form of plantaricins E (PlnE) and F (PlnF). This study emphasizes the importance of genome sequencing and bioinformatic analysis for evaluating aquaculture probiotic candidates. Moreover, it provides valuable insights into their genetic features and antimicrobial mechanisms, paving the way for their application as probiotics in larviculture, which is a major bottleneck in aquaculture.

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Comparative Probiogenomics Analysis of Limosilactobacillus fermentum 3872

Ullah,

Rizwan,

Han

et al. 2024

Probiotics & Antimicro. Prot.

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Functional genomic characterization unveils probiotic features of G1-11 isolated from the gut of the hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂)

Cited by 7 publications

References 40 publications

Biodegradation of polyethylene (PE), polypropylene (PP), and polystyrene (PS) microplastics by floc-forming bacteria, Bacillus cereus strain SHBF2, isolated from a commercial aquafarm

Biodegradation of polyethylene (PE), polypropylene (PP), and polystyrene (PS) microplastics by floc-forming bacteria, Bacillus cereus strain SHBF2, isolated from a commercial aquafarm

Genomic and Functional Evaluation of Two Lacticaseibacillus paracasei and Two Lactiplantibacillus plantarum Strains, Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis), as Probiotics for Aquaculture

Comparative Probiogenomics Analysis of Limosilactobacillus fermentum 3872

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