Functional gap junctions in mouse small intestinal crypts

Bjerknes, Matthew; Cheng, Hazel; Erlandsen, Stanley L.

doi:10.1002/ar.1092120407

Cited by 34 publications

(10 citation statements)

References 21 publications

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“…In addition to the junctional complexes, the lateral membranes interact by means of cell adhesion molecules, gap junctions, and interdigitations. Gap junctions, which are located in the basolateral membrane in other epithelial cells and directly mediate cell-to-cell communication, also have been identified in intestinal crypt cells (Bjerknes et al, 1985). The basolateral membrane, which begins below the junctional complex, contains abundant Na + , K + -ATPase and adenylate cyclase and differs from the apical membrane in function.…”

Section: Structure Of the Absorptive Epitheliummentioning

confidence: 99%

M Cells

Williams

Owen

2015

Mucosal Immunology

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Section: Structure Of the Absorptive Epitheliummentioning

confidence: 99%

M Cells

Williams

Owen

2015

Mucosal Immunology

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“…The growth environment we introduced in the new model is a gradient of a stimulatory substance diffusing from the bottom of the crypt to the top. We were in fact inspired by observations of Bjerknes et al [1985] about the existence of intraepithelial gap junctions that would permit the passage of molecules from columnar cell to columnar cell. We assume that the growth factor degrade with time.…”

Section: Modeling the Intestinal Epitheliummentioning

confidence: 99%

Tissue Stem Cells: Definition, Plasticity, Heterogeneity, Self-Organization and Models – A Conceptual Approach

Loeffler

Roeder

2002

Cells Tissues Organs

164

114

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The classical definition of adult tissue stem cells (TSC) is fundamentally based on a functional perspective. A TSC is an undifferentiated cell, capable of proliferation, self-renewal, production of a large number of differentiated functional progeny, regenerating tissue after injury and a flexibility in the use of these options. Here, we discuss the necessity for amending this definition in the light of recent insight into stem cell biology regarding stem cell heterogeneity, lineage plasticity, clonal fluctuation and cell–environment interactions. We conclude that the definition needs amendments. A decade ago the flexibility criterion has attracted little attention but recent findings have indicated its importance. Flexibility and reversibility of tissue and lineage specification (tissue plasticity) and of properties within a tissue (within-tissue plasticity) have major implications with regard to concepts of stem cell function. We advocate to give up the view of TSC as being entities with a preprogrammed development and to replace it by a concept that makes the capabilities for flexible and regulated tissue self-organization based on cell–cell and cell–environment interactions the new paradigm. This concept would permit to incorporate the context-dependent lineage plasticity, within-lineage plasticity and generation of stem cell heterogeneity as a result of a dynamically regulated process. Such concepts need a rigorous examination by formal modeling including simulation studies. We provide some general ideas on how to proceed with such theories and illustrate this with worked models for tissue stem cells of the hematopoietic system and the intestinal epithelium.

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“…The functional importance of gap junctions in the intestine and their possible role in tumorigenesis have been poorly characterised, although gap junctions are found both in the epithelial and muscular tissues. 4,5 One of the main and very early events in intestinal carcinogenesis is truncating mutations in the tumour suppressor gene adenomatous polyposis coli (APC). Mutations in APC are both involved in inheritable and spontaneous forms of intestinal cancer, where >80% of sporadic adenomas have mutations in APC.…”

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confidence: 99%

Connexin43 is overexpressed in Apc^Min/+‐mice adenomas and colocalises with COX‐2 in myofibroblasts

Husøy

Knutsen

Cruciani

et al. 2005

Intl Journal of Cancer

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The expression of gap junction proteins, connexins, in the intestine and their role in tumorigenesis are poorly characterised. Truncating mutations in the tumour suppressor gene adenomatous polyposis coli (APC) are early and important events, both in inheritable (familial adenomatous polyposis, FAP) and spontaneous forms of intestinal cancer. Multiple intestinal neoplasia (Min) mice, a FAP model with inherited heterozygous mutation in Apc, spontaneously develop numerous intestinal adenomas. We recently reported reduced expression of connexin32 in Paneth cells of Min-mice. We further examine the expression of connexin43 (Cx43) and other connexins as a function of heterozygous and homozygous Apc mutation in normal intestinal tissues and adenomas of Min-mice. Qualitative analysis of connexin mRNA in intestine revealed a similar expression pattern in Min-and wildtype (wt) mice. Connexin26 and connexin40 proteins were found in equal amounts in Min and wt epithelia of large and small intestine, respectively. Interestingly, the connexin43 level was increased in the stroma of Min-mice adenomas, in close proximity to epithelial cells with nuclear b-catenin staining. Cx43 and COX-2 were located to the same areas of the adenomas, and immunostaining exhibited coexpression in the myofibroblasts. Prostaglandin E2 induces Cx43 expression and COX-2 is the rate-limiting enzyme in the prostaglandin synthesis. However, the COX-2-specific inhibitor, celecoxib, did not reduce Cx43 expression. Although both Cx43 and COX-2 are target genes for b-catenin, they were overexpressed in stromal cells but not in epithelial tumour cells. We hypothesise that gap junctions may be of importance in the transfer of signals between epithelium and stroma. ' 2005 Wiley-Liss, Inc.

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Functional gap junctions in mouse small intestinal crypts

Cited by 34 publications

References 21 publications

M Cells

M Cells

Tissue Stem Cells: Definition, Plasticity, Heterogeneity, Self-Organization and Models – A Conceptual Approach

Connexin43 is overexpressed in Apc^Min/+‐mice adenomas and colocalises with COX‐2 in myofibroblasts

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Functional gap junctions in mouse small intestinal crypts

Cited by 34 publications

References 21 publications

M Cells

M Cells

Tissue Stem Cells: Definition, Plasticity, Heterogeneity, Self-Organization and Models – A Conceptual Approach

Connexin43 is overexpressed in ApcMin/+‐mice adenomas and colocalises with COX‐2 in myofibroblasts

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Connexin43 is overexpressed in Apc^Min/+‐mice adenomas and colocalises with COX‐2 in myofibroblasts