Functional Fermented Milk with Fruit Pulp Modulates the In Vitro Intestinal Microbiota

Abstract

The effect of putative probiotic fermented milk (FM) with buriti pulp (FMB) or passion fruit pulp (FMPF) or without fruit pulp (FMC) on the microbiota of healthy humans was evaluated. FM formulations were administered into a simulator of the human intestinal microbial ecosystem (SHIME®) to evaluate the viability of lactic acid bacteria (LAB), microbiota composition, presence of short-chain fatty acids (SCFA), and ammonium ions. The probiotic LAB viability in FM was affected by the addition of the fruit pulp. Phocaeicola was dominant in the FMPF and FMB samples; Bifidobacterium was related to FM formulations, while Alistipes was associated with FMPF and FMB, and Lactobacillus and Lacticaseibacillus were predominant in FMC. Trabulsiella was the central element in the FMC, while Mediterraneibacter was the central one in the FMPF and FMB networks. The FM formulations increased the acetic acid, and a remarkably high amount of propionic and butyric acids were detected in the FMB treatment. All FM formulations decreased the ammonium ions compared to the control; FMPF samples stood out for having lower amounts of ammonia. The probiotic FM with fruit pulp boosted the beneficial effects on the intestinal microbiota of healthy humans in addition to increasing SCFA in SHIME® and decreasing ammonium ions, which could be related to the presence of bioactive compounds.

Influence of incorporating dried fruits on dairy drinks characteristics focusing on their antimicrobial effects

“…This log reduction may be attributed to the acid output of dried fruit extracts. Also, the starter bacteria’s presence may be an additional suppression of Rayeb milk spoilage [ 27 ]. It is also an added value to produce new fortified milk products that are acceptable to consumers at suitable prices.…”

Dietary probiotic Lacticaseibacillus paracasei NSMJ56 modulates gut immunity and microbiota in laying hens

“…The elevated genera by dietary NSMJ56 were Flintibacter, Dielma , and Hespellia followed by Coprobacter, Roseburia, Anaerotignum , and Coprococcus. Flintibacter is known to produce SCFA, such as butyrate and constitutes a component of the beneficial microbiota ( Borgonovi et al, 2022 ). Qiu et al (2022) showed that the relative abundance of Dielma genera was positively correlated with SCFAs production.…”

Evidence for the Beneficial Effects of Brazilian Native Fruits and Their By-Products on Human Intestinal Microbiota and Repercussions on Non-Communicable Chronic Diseases—A Review

“…A high Firmicutes to Bacteroidetes ratio in intestinal microbiota is a typical alteration in individuals with obesity and related diseases [ 44 , 45 ], since Firmicutes could be more effective in extracting energy, especially from carbohydrates, thereby affecting the energetic metabolism [ 46 ]. Increases in Proteobacteria abundance in the intestine have been commonly found in individuals with obesity [ 46 , 47 , 48 ]. Proteobacteria is among the most abundant intestinal microbiota phyla and includes various pathogenic microorganisms (e.g., Enterobacteriaceae members).…”

“…Berg), juçara ( Euterpe edulis Mart. ), and passion fruit ( Passiflora capsularis L.) are widely cultivated and available in Brazil; they are reported as sources of bioactive components that are capable of modulating the composition and metabolic activity of the intestinal microbiota with local and systemic benefits, including anti-inflammatory and antioxidant activity, better insulin sensitivity, body weight reduction, and effective management of dyslipidemia, besides having evidence of low genotoxic [ 10 , 15 , 33 , 44 , 48 , 54 , 81 , 87 ]. Table 1 summarizes the major bioactive compounds found in peel, seed, and/or pulp of Brazilian native fruits that are being investigated for their ability to modulate intestinal microbiota.…”

“…Clostridiaceae family could play a role in providing energy for colonocytes and protecting the epithelial barrier. Furthermore, acetic, propionic, and butyric acid contents were higher in fermented milk supplemented with the buriti pulp, suggesting it as a substrate that supports probiotic growth, besides modulating the intestinal microbiota and SCFA production [ 48 ]. Fresh and freeze-dried buriti pulp and endocarp are sources of insoluble and soluble dietary fiber, including soluble pectin, as well as phenolic compounds, such as isoquercetin, ferrulic acid, vanillic acid, caffeic acid, and quercetin, which could account for the reported effects on intestinal microbiota [ 11 , 88 , 89 ].…”