Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes in<i>Rhodococcus</i>Species for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated Dioxins

Iida, Toshiya; Moteki, Yasuhiro; Nakamura, Kaoru; Taguchi, Katsuhisa; Otagiri, Masato; Asanuma, Miwako; Dohmae, Naoshi; Usami, Ron; Kudo, Toshiaki

doi:10.1271/bbb.80680

Cited by 5 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RD2 and R. erythropolis TA422, JCM 2892, and JCM 3201 under the control of the constitutive promoter P dfdB . These enzymes participated in oxidative degradation of dibenzofuran and chlorinated dioxins and were difficult to obtain in classical E. coli expression systems [129].…”

Section: Genetic Modifications Of Rhodococcusmentioning

confidence: 99%

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

2019

View full text Add to dashboard Cite

The review is devoted to biocatalysts based on actinobacteria of the genus Rhodococcus, which are promising for environmental biotechnologies. In the review, biotechnological advantages of Rhodococcus bacteria are evaluated, approaches used to develop robust and efficient biocatalysts are discussed, and their relevant applications are given. We focus on Rhodococcus cell immobilization in detail (methods of immobilization, criteria for strains and carriers, and optimization of process parameters) as the most efficient approach for stabilizing biocatalysts. It is shown that advanced Rhodococcus biocatalysts with improved working characteristics, enhanced stress tolerance, high catalytic activities, human and environment friendly, and commercially viable are developed, which are suitable for wastewater treatment, bioremediation, and biofuel production.

show abstract

Section: Genetic Modifications Of Rhodococcusmentioning

confidence: 99%

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

2019

View full text Add to dashboard Cite

show abstract

Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans

Kasuga

Nitta

Kobayashi

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

A dibenzofuran (DF)-degrader Terrabacter sp. strain DBF63 harbors the dbfA and dbfBC genes for DF degradation and the fln-dbfA, pht, and pca gene clusters for the utilization of fluorene (FN) as a sole carbon source. From this strain, dfdA1, the gene encoding the second DF dioxygenase was detected using degenerate polymerase chain reaction (PCR) and the dfdA1A2A3A4 genes were cloned from a cosmid library of the DBF63 genome. Nucleotide sequencing revealed that the dfdA genes showed considerably high identities with those of other actinobacteria, such as Terrabacter sp. strain YK3 and Rhodococcus sp. strain HA01. In the neighboring region of the dfdA genes, as many as 11 homologs for transposase and integrase genes and the putative extradiol dioxygenase gene disrupted by an insertion sequence (dfdB::ISTesp2) were found, suggesting that repeated gene rearrangement had occurred. Quantitative reverse transcription-PCR analysis revealed that dfdA1 was expressed primarily in the DF-fed strain, whereas dbfA1 was expressed in the FN-cultured strain, apparently indicating that the dfdA genes are induced by DF for the initial hydroxylation of DF in strain DBF63. Furthermore, two polycistronic gene cassettes consisting of either dfdA or dbfA together with the dbfBC gene were constructed and expressed heterologously in Streptomyces lividans, degrading DF to salicylate. Furthermore, the expressed DfdA dioxygenase degraded dibenzo-p-dioxin, carbazole, dibenzothiophene, anthracene, phenanthrene, and biphenyl, thereby exhibiting a broader substrate range than that of the DbfA dioxygenase.

show abstract

Preparation of reductases for multicomponent oxygenases

Wolf,

Eltis

2024

Methods in Enzymology

View full text Add to dashboard Cite

Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes inRhodococcusSpecies for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated Dioxins

Cited by 5 publications

References 43 publications

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans

Preparation of reductases for multicomponent oxygenases

Contact Info

Product

Resources

About