Functional Expression of Sv40 in Normal Human Prostatic Epithelial and Fibroblastic Cells - Differentiation Pattern of Nontumorigenic Cell-Lines

Berthon, Philippe; Cussenot, Olivier; Hopwood, Larry E.; Leduc, Andrew; Nj, Maitland

doi:10.3892/ijo.6.2.333

Cited by 74 publications

(87 citation statements)

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“…The transgene was found to be exclusively expressed in the glandular epithelium of both prostates (Figure 2). These observations con®rm our in vitro data generated by infection of primary cultures of human epithelial and ®broblastic cells, as well as of human epithelial prostatic cell lines PNT1A and PNT2 24 with AdRSVbgal, and our in vivo experiments on human prostatic adenocarcinoma grafted in nude mice (data not shown). Knowing that more than 95% of human prostate cancer are derived from glandular epithelium, 25 this selective transduction of epithelial cells by a recombinant adenovirus makes this viral vector an attractive candidate for treatment of prostatic adenocarcinoma by gene transfer.…”

Section: Discussionsupporting

confidence: 86%

Adenovirus-mediated gene transfer in dog prostate: a preclinical study of a relevant model system for gene therapy of human prostatic cancer

Andrawiss¹,

Opolon²,

Benihoud³

et al. 1999

Prostate Cancer Prostatic Dis

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This present study evaluates the potential of adenovirus-mediated gene transfer (AMGT) to the prostate of normal laboratory beagles. Many morphological and histological similarities can be noted between dog and human prostate. Moreover, dogs can spontaneously develop prostate cancer with a clinical and biological outcome identical to that in man. Firstly we showed the capacity of human adenovirus to infect canine prostatic cells in vitro. Secondly, we injected transrectally in the dogs' prostates 2610 9 plaque forming units of a ®rst generation recombinant adenovirus vector harboring the reporter gene b-galactosidase (AdRSVbgal). Seven days after the adenoviral delivery, we observed expression of the transgene in both prostates, and exclusively in epithelial cells. Despite a cellular and a humoral immune response, the infusion appeared safe, since the dogs had no fever and presented no urinary symptoms. This study constitutes the ®rst evaluation of AMGT in dog prostate and provides a basis for gene therapy treatment of prostate carcinoma-bearing patients.

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Section: Discussionsupporting

confidence: 86%

Adenovirus-mediated gene transfer in dog prostate: a preclinical study of a relevant model system for gene therapy of human prostatic cancer

Andrawiss¹,

Opolon²,

Benihoud³

et al. 1999

Prostate Cancer Prostatic Dis

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View full text Add to dashboard Cite

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“…In the prostate cell lines examined, expression levels in LNCaP and PC3 cells (derived from prostate metastases; Kaighn et al, 1979;Horoszewicz et al, 1983) were approximately one-fifth those in PNT2C2, P4E6 and 22Rv1 cells (derived from normal prostate cells or primary prostate cancer cells; Berthon et al, 1995;Sramkoski et al, 1999;Maitland et al, 2001). Furthermore, in screens of clonogenic activity with a range of chemotherapeutic agents and UV irradiation, we have found that, in general, PNT2C2 and P4E6 cells show high sensitivity, 22Rv1 cells show intermediate sensitivity and PC3 and LNCaP cells show high resistance.…”

Section: Discussionmentioning

confidence: 99%

GAS5, a non-protein-coding RNA, controls apoptosis and is downregulated in breast cancer

et al. 2008

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Effective control of both cell survival and cell proliferation is critical to the prevention of oncogenesis and to successful cancer therapy. Using functional expression cloning, we have identified GAS5 (growth arrest-specific transcript 5) as critical to the control of mammalian apoptosis and cell population growth. GAS5 transcripts are subject to complex post-transcriptional processing and some, but not all, GAS5 transcripts sensitize mammalian cells to apoptosis inducers. We have found that, in some cell lines, GAS5 expression induces growth arrest and apoptosis independently of other stimuli. GAS5 transcript levels were significantly reduced in breast cancer samples relative to adjacent unaffected normal breast epithelial tissues. The GAS5 gene has no significant protein-coding potential but expression encodes small nucleolar RNAs (snoRNAs) in its introns. Taken together with the recent demonstration of tumor suppressor characteristics in the related snoRNA U50, our observations suggest that such snoRNAs form a novel family of genes controlling oncogenesis and sensitivity to therapy in cancer.

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“…The prostate cell lines PC-3 (Kaighn et al, 1979) and PNT2-C2 (Berthon et al, 1995) were cultured in Ham's F12, 7% FCS and 2 mM L-glutamine and in RPMI 1640, 10% FCS and 2 mM Lglutamine, respectively. PC3-GFP were cultured as standard PC-3 cells but with the addition of Hygromycin B (0.15 mg ml À1 ) (Sharrard and Maitland, 2000).…”

Section: Cell Linesmentioning

confidence: 99%

Invasive characteristics of human prostatic epithelial cells: understanding the metastatic process

et al. 2005

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Prostate cancer has a predilection to metastasise to the bone marrow stroma (BMS) by an as yet uncharacterised mechanism. We have defined a series of coculture models of invasion, which simulate the blood/BMS boundary and allow the elucidation of the signalling and mechanics of trans-endothelial migration within the complex bone marrow environment. Confocal microscopy shows that prostate epithelial cells bind specifically to bone marrow endothelial-to-endothelial cell junctions and initiate endothelial cell retraction. Trans-endothelial migration proceeds via an epithelial cell pseudopodial process, with complete epithelial migration occurring after 232743 min. Stromal-derived factor-1 (SDF-1)/CXCR4 signalling induced PC-3 to invade across a basement membrane although the level of invasion was 3.5-fold less than invasion towards BMS (P ¼ 0.0007) or bone marrow endothelial cells (P ¼ 0.004). Maximal SDF-1 signalling of invasion was completely inhibited by 10 mM of the SDF-1 inhibitor T140. However, 10 mM T140 only reduced invasion towards BMS and bone marrow endothelial cells by 59% (P ¼ 0.001) and 29% (P ¼ 0.011), respectively. This study highlights the need to examine the potential roles of signalling molecules and/or inhibitors, not just in single-cell models but in coculture models that mimic the complex environment of the bone marrow.

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Functional Expression of Sv40 in Normal Human Prostatic Epithelial and Fibroblastic Cells - Differentiation Pattern of Nontumorigenic Cell-Lines

Cited by 74 publications

References 0 publications

Adenovirus-mediated gene transfer in dog prostate: a preclinical study of a relevant model system for gene therapy of human prostatic cancer

Adenovirus-mediated gene transfer in dog prostate: a preclinical study of a relevant model system for gene therapy of human prostatic cancer

GAS5, a non-protein-coding RNA, controls apoptosis and is downregulated in breast cancer

Invasive characteristics of human prostatic epithelial cells: understanding the metastatic process

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