Functional expression of HLA-DP genes transfected into mouse fibroblasts

Austin, Penelope; Trowsdale, John; Rudd, Christopher E.; Bodmer, W F; Feldmann, Marc; Lamb, Jonathan R.

doi:10.1038/313061a0

Cited by 95 publications

(32 citation statements)

References 24 publications

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“…It was further demonstrated that SB/HLA-DP, when transfected into murine fibroblasts, could present influenza viral peptides to DP-restricted human T cells (48). There are a growing number of examples of viral peptides that bind to DP molecules and elicit CD4 ϩ T cell responses, particularly for DP4, the allele used in this study.…”

Section: Discussionmentioning

confidence: 99%

HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM

Lith

McEwen-Smith

Benham

2010

Journal of Biological Chemistry

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The MHC is central to the adaptive immune response. The human MHC class II is encoded by three different isotypes, HLA-DR, -DQ, and -DP, each being highly polymorphic. In contrast to HLA-DR, the intracellular assembly and trafficking of HLA-DP molecules have not been studied extensively. However, different HLA-DP variants can be either protective or risk factors for infectious diseases (e.g. hepatitis B), immune dysfunction (e.g. berylliosis), and autoimmunity (e.g. myasthenia gravis). Here, we establish a system to analyze the chaperone requirements for HLA-DP and to compare the assembly and trafficking of HLA-DP, -DQ, and -DR directly. Unlike HLA-DR1, HLA-DQ5 and HLA-DP4 can form SDS-stable dimers supported by invariant chain (Ii) in the absence of HLA-DM. Uniquely, HLA-DP also forms dimers in the presence of HLA-DM alone. In model antigen-presenting cells, SDS-stable HLA-DP complexes are resistant to treatments that prevent formation of SDS-stable HLA-DR complexes. The unexpected properties of HLA-DP molecules may help explain why they bind to a more restricted range of peptides than other human MHC class II proteins and frequently present viral peptides.

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Section: Discussionmentioning

confidence: 99%

HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM

Lith

McEwen-Smith

Benham

2010

Journal of Biological Chemistry

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“…It has been shown that mouse fibroblasts transfected with the genes of the murine la or human DR polypeptides can express the molecules on the cell surface. Moreover, these Ia-expressing transfectants are capable of stimulating several antigen-and allo-specific T cells in an MHC-restricted manner (5)(6)(7)(8).…”

mentioning

confidence: 99%

“…Recent advances in cloning the genes of the a and p subunits of the Ta molecule and their expression in cells that normally do not express Ia gene products have allowed a direct approach to the study of the structure-function relationships of the Ta molecule (3)(4)(5)(6)(7)(8). It has been shown that mouse fibroblasts transfected with the genes of the murine la or human DR polypeptides can express the molecules on the cell surface.…”

mentioning

confidence: 99%

Ia-transfected L-cell fibroblasts present a lysozyme peptide but not the native protein to lysozyme-specific T cells.

Shastri¹,

Malissen²,

Hood³

1985

Proc. Natl. Acad. Sci. U.S.A.

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“…Four different mouse monoclonal antibodies against class II antigens were applied as the first antibody. Anti-HLA-DR (IgG2a, Becton Dickinson, Mountain View, CA, USA) (Wang et al 1983) and Nu-la (IgGI, Nichirei, Tokyo) (Matsumoto 1984) were used as the anti-HLA-DR antibody, anti-HLA-DP (IgG2a, Becton Dickinson) (Austin et al 1985) was used as the anti-HLA-DP antibody, and Leu10 (IgG2a, Becton Dickinson) (Chen et al 1989) was used as the anti-HLA-DQ antibody. Two monoclonal antibodies against anti-keyhole limpet hemocyanin, clone x 39 (IgG2a) and x 40 (IgGI) (Becton Dickinson) were used as monoclonal controls.…”

Section: Methodsmentioning

confidence: 99%

“…It has been shown that HLA-DR antigens are required for antigen-recognition of helper T cells, and that HLA-DQ antigens are required for antigen-recognition of suppressor T cells (Hirayama et al 1987; Matsushita et al 1987). HLA-DP antigens have been shown to be required for antigen-recognition of helper T cells in cooperation with HLA-DR antigens (Eckels et al 1983; Austin et al 1985). Namely, HLA-DR with or without HLA-DP, and HLA-DQ antigens seem to restrict the reverse immune-response to each other.…”

mentioning

confidence: 99%

Class II (HLA-DR, HLA-DP, and HLA-DQ) Antigens on Colonic Epithelia in Ulcerative Colitis: A Comparison between Uneventful and Intractable Cases.

Horie

Chiba

Iizuka

et al. 1991

Tohoku J. Exp. Med.

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HORIE, Y., CHIBA, M., IIzuKA, M. and MASAMUNE, 0. Class II (HLA-DR, HLA-DP, and HLA-DQ) Antigens on Colonic Epithelia in Ulcerative Colitis : A Comparison between Uneventful and Intractable Cases. Tohoku J. Exp. Med., 1991, 165 (2), 87-97 Class II antigens on colonic epithelia involved in ulcerative colitis (UC) with uneventful or intractable courses were investigated using an immunoperoxidase method. Twenty normal colonic mucosa served as normal controls. Nineteen specimens from 14 uneventful cases and 23 specimens from 12 intractable cases were studied. Normal colonic epithelia did not express class II antigens. In UC, there were no differences between uneventful cases and intractable cases in the frequencies of class II antigen expression, the distribution of class II antigen expression ratio, or the mutual relationship of the extent of expression of the three class II antigens on colonic epithelia. However, while the extent of expression of HLA-DR and HLA-DP antigens on the epithelia was positively correlated with the degree of inflammation in uneventful cases, there was no such correlation in intractable cases. These observations may suggest that the induction mechanisms of class II antigens on colonic epithelia in UC differ in uneventful and intractable cases.class II (HLA-DR, HLA-DP, and HLA-DQ) antigens ; ulcerative colitis ; uneventful case ; intractable case ; immunology There are various clinical courses in ulcerative colitis (UC). Some cases take an uneventful course, while some others take a poor course. Uneventful cases easily lead to a remission and this may continue for a long time. However, cases which follow a poor course do not easily lead to a remission and, even if remission is obtained, relapse occurs within a short time. These latter cases are refered to as intractable cases. In our Department, about half of the intractable cases have surgical intervention ). There are also intermediate cases between uneventful and intractable cases. At present, factors defining the clini-

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Functional expression of HLA-DP genes transfected into mouse fibroblasts

Cited by 95 publications

References 24 publications

HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM

HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM

Ia-transfected L-cell fibroblasts present a lysozyme peptide but not the native protein to lysozyme-specific T cells.

Class II (HLA-DR, HLA-DP, and HLA-DQ) Antigens on Colonic Epithelia in Ulcerative Colitis: A Comparison between Uneventful and Intractable Cases.

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