Functional expression of Ca2+ signaling pathways in mouse embryonic stem cells

Yanagida, Eri; Shoji, Shuichi; Hirayama, Yoshiyuki; Yoshikawa, Fumio; Otsu, Keishi; Uematsu, Hiroshi; Hiraoka, Masayasu; Furuichi, Teiichi; Kawano, Seiko

doi:10.1016/j.ceca.2004.01.022

Cited by 70 publications

(81 citation statements)

References 45 publications

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“…Recent studies showed that the expression level of IP 3 R3 increases after fertilization in zebrafish, 12 indicating a possible involvement of IP 3 R3 in the regulation of Ca 2 þ signaling during early embryo development. IP 3 R3 is also detected in mES cells 23,24 and it has been shown to mediate Ca 2 þ oscillations related to cell cycle regulation. 24 However, little is known regarding the exact function of IP 3 R3 in undifferentiated and differentiating ES cells.…”

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confidence: 99%

Type 3 inositol 1,4,5-trisphosphate receptor negatively regulates apoptosis during mouse embryonic stem cell differentiation

Tang

et al. 2010

Cell Death Differ

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Ca 2 þ signals generated by inositol 1,4,5-trisphosphate receptors (IP 3 Rs) are crucial for cellular processes such as apoptosis and differentiation. However, the exact roles of IP 3 Rs and their contributions to Ca 2 þ signals in pluripotent embryonic stem (ES) cell behaviors remain largely unknown. In this study, we showed that the expression of type 3 IP 3 R (IP 3 R3) was transiently downregulated with a concomitant increase in apoptosis at the early differentiation stage of murine ES cells. Knockdown of IP 3 R3 by small interfering RNA increased apoptosis in differentiating cells but not in undifferentiated ES cells. Moreover, IP 3 R3 overexpression had the opposite effect. Consistently, IP 3 R3 knockdown altered Ca 2 þ oscillations in differentiating cells but not in undifferentiated ES cells. The apoptosis in differentiating IP 3 R3-knockdown cells was decreased by chelating intracellular Ca 2 þ with BAPTA-AM and increased in control ones. Furthermore, IP 3 R3 knockdown led to a suppression of the expression of mesodermal and mesoendodermal but not ectodermal markers. The differentiation suppressions were further confirmed by the impaired differentiation of mesodermal and some of the endodermal but not ectodermal derivatives. Such defects were partially because of the increased apoptosis in Flk-1 þ cells. These findings provide the first demonstration of the important role of IP 3 R3 in the regulation of apoptosis in early differentiating ES cells and subsequent lineage commitments through modulation of Ca 2 þ signals.

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confidence: 99%

Type 3 inositol 1,4,5-trisphosphate receptor negatively regulates apoptosis during mouse embryonic stem cell differentiation

Tang

et al. 2010

Cell Death Differ

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“…Calcium signals in hESC-derived cardiomyocytes and neural cell types have been measured in most cases by calcium sensitive dyes: for cardiomyocytes Fura-2/AM [25][26][27][28], Fluo-4/AM [29,30] or dsRed+ [27], while for neural cell types Fluo-3/AM [8,31] Fura-2/AM [8,32] or Oregon green 488 Bapta-1 AM [33] have been applied. In pluripotent cells the application of Fluo-3/AM (mESC- [34], hESC- [8]) or Fluo-4/AM (mESC- [35][36][37], and hESC [12]) has been reported. The application of GECIs, while already widely reported in various functional studies, has only been described in mPSCs [38] and in hPSCs, as well as in their differentiated offsprings (see Apati et al [39]).…”

Section: Methods For Studying Calcium Signals In Human Ps Cellsmentioning

confidence: 99%

Calcium signaling in human pluripotent stem cells

2016

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a b s t r a c tHuman pluripotent stem cells provide new tools for developmental and pharmacological studies as well as for regenerative medicine applications. Calcium homeostasis and ligand-dependent calcium signaling are key components of major cellular responses, including cell proliferation, differentiation or apoptosis. Interestingly, these phenomena have not been characterized in detail as yet in pluripotent human cell sates. Here we review the methods applicable for studying both short-and long-term calcium responses, focusing on the expression of fluorescent calcium indicator proteins and imaging methods as applied in pluripotent human stem cells. We discuss the potential regulatory pathways involving calcium responses in hPS cells and compare these to the implicated pathways in mouse PS cells. A recent development in the stem cell field is the recognition of so called "naïve" states, resembling the earliest potential forms of stem cells during development, as well as the "fuzzy" stem cells, which may be alternative forms of pluripotent cell types, therefore we also discuss the potential role of calcium homeostasis in these PS cell types.

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“…29 However, in another study only PMCA1 and PMCA4 expression were detected in mouse ESCs. 31 These differences may be due to the methodological approaches used in each study.…”

Section: Pmca Expression In the Early Stages Of Liver Developmentmentioning

confidence: 99%