Functional expression of an scFv on bacterial magnetic particles by in vitro docking

Sugamata, Yasuhiro; Tanaka, Tsuyoshi; Matsunaga, Tadashi; Yoshino, Tomoko

doi:10.1016/j.bbrc.2013.12.102

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…Therefore, MTB have emerged as excellent model organisms to study the biogenesis of bacterial organelles, biomineralization, protein targeting, and bacterial cell biology. In addition, magnetosomes have been genetically engineered with respect to both their magnetite core as well their enveloping membrane, and numerous applications of functionalized magnetosomes have been demonstrated (5)(6)(7)(8). However, the progress in exploring the biology of MTB and in engineering magnetosomes has been impeded by the limited genetic tools available for these fastidious bacteria.…”

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confidence: 99%

A Tailored galK Counterselection System for Efficient Markerless Gene Deletion and Chromosomal Tagging in Magnetospirillum gryphiswaldense

Raschdorf

Plitzko

Schüler

et al. 2014

Appl Environ Microbiol

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cMagnetotactic bacteria have emerged as excellent model systems to study bacterial cell biology, biomineralization, vesicle formation, and protein targeting because of their ability to synthesize single-domain magnetite crystals within unique organelles (magnetosomes). However, only few species are amenable to genetic manipulation, and the limited methods for site-specific mutagenesis are tedious and time-consuming. Here, we report the adaptation and application of a fast and convenient technique for markerless chromosomal manipulation of Magnetospirillum gryphiswaldense using a single antibiotic resistance cassette and galK-based counterselection for marker recycling. We demonstrate the potential of this technique by genomic excision of the phbCAB operon, encoding enzymes for polyhydroxyalkanoate (PHA) synthesis, followed by chromosomal fusion of magnetosome-associated proteins to fluorescent proteins. Because of the absence of interfering PHA particles, these engineered strains are particularly suitable for microscopic analyses of cell biology and magnetosome biosynthesis.

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mentioning

confidence: 99%

A Tailored galK Counterselection System for Efficient Markerless Gene Deletion and Chromosomal Tagging in Magnetospirillum gryphiswaldense

Raschdorf

Plitzko

Schüler

et al. 2014

Appl Environ Microbiol

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“…S [96] -in vitro k ‖ the difficulties associated with expressing disulfide bond-containing proteins. The affinity partner Fc-fused disulfide-bonded protein was expressed in the periplasm, while its binding partner Mms13-ZZ was expressed on the magnetosome.…”

Section: Magnetosome Fabrications To Datementioning

confidence: 99%

Recombinant surface engineering to enhance and expand the potential of biologically produced nanoparticles: A review

Kutralam-Muniasamy

Pérez-Guevara

2017

Process Biochemistry

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“…Other practical applications of magnetosomes includes as magnetic resonance imaging (MRI) probe or modified with protein for tumor detection [57,58], the tracking of stem cells, dendritic cells and single nucleotide polymorphism (SNP) [59,60], detection of pathogenic bacteria, viruses [61][62][63] and other immunodetection [64,65], discrimination or recovery of DNA and mRNA [59,[66][67][68], adsorption and mineralization of metals [69][70][71][72][73][74] and known as the powerful tool for chip-based whole-cell biosensors [75]. "Surface display system" of magnetosomes used in immunoassay, enzyme reaction, and cell separation [76][77][78][79][80][81][82][83][84][85][86][87]. Magnetosomes can also be used as an effective model system to study CDF related II type diabetes [88].…”

Section: Applicationsmentioning

confidence: 99%

Improved methods for mass production of magnetosomes and applications: a review

et al. 2020

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Magnetotactic bacteria have the unique ability to synthesize magnetosomes (nano-sized magnetite or greigite crystals arranged in chain-like structures) in a variety of shapes and sizes. The chain alignment of magnetosomes enables magnetotactic bacteria to sense and orient themselves along geomagnetic fields. There is steadily increasing demand for magnetosomes in the areas of biotechnology, biomedicine, and environmental protection. Practical difficulties in cultivating magnetotactic bacteria and achieving consistent, high-yield magnetosome production under artificial environmental conditions have presented an obstacle to successful development of magnetosome applications in commercial areas. Here, we review information on magnetosome biosynthesis and strategies for enhancement of bacterial cell growth and magnetosome formation, and implications for improvement of magnetosome yield on a laboratory scale and mass-production (commercial or industrial) scale.

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Functional expression of an scFv on bacterial magnetic particles by in vitro docking

Cited by 9 publications

References 30 publications

A Tailored galK Counterselection System for Efficient Markerless Gene Deletion and Chromosomal Tagging in Magnetospirillum gryphiswaldense

A Tailored galK Counterselection System for Efficient Markerless Gene Deletion and Chromosomal Tagging in Magnetospirillum gryphiswaldense

Recombinant surface engineering to enhance and expand the potential of biologically produced nanoparticles: A review

Improved methods for mass production of magnetosomes and applications: a review

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