Functional expression and characterization of a recombinant phospholipase A2 from sea snake Lapemis hardwickii as a soluble protein in E. coli

Wen, Yunfei; Li, Peng; Zhong, Xingwu; Wei, Jian Wen; Jiang, Xiao; Ye, Lan Ting; Zou, Li; Tu, Hong Bin; Wu, Wen Yan; An, Xiaobo

doi:10.1016/s0041-0101(03)00047-3

Cited by 21 publications

(9 citation statements)

References 30 publications

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“…Likewise, the PLA 2 peptide sequences obtained are closely related to those of basic enzymes previously reported in these snake genera [57][58][59][60].…”

Section: Accepted Manuscriptsupporting

confidence: 76%

Two color morphs of the pelagic yellow-bellied sea snake, Pelamis platura, from different locations of Costa Rica: Snake venomics, toxicity, and neutralization by antivenom

Lomonte

Plá

Sasa

et al. 2014

Journal of Proteomics

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“…Likewise, the PLA 2 peptide sequences obtained are closely related to those of basic enzymes previously reported in these snake genera [57][58][59][60].…”

Section: Accepted Manuscriptsupporting

confidence: 76%

Two color morphs of the pelagic yellow-bellied sea snake, Pelamis platura, from different locations of Costa Rica: Snake venomics, toxicity, and neutralization by antivenom

Lomonte

Plá

Sasa

et al. 2014

Journal of Proteomics

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“…However, a more likely explanation is that inhibition was caused by Type I PLA 2 s, which are well known from elapid venoms [216,217,218,219,220,221], and that are able to inhibit aggregation induced by ADP, collagen, and epinephrine. Depending upon the species and the PLA 2 in question, this inhibition may involve catalytic or non-catalytic mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Coralsnake Venomics: Analyses of Venom Gland Transcriptomes and Proteomes of Six Brazilian Taxa

Aird

Silva

Qiu

et al. 2017

Toxins

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Venom gland transcriptomes and proteomes of six Micrurus taxa (M. corallinus, M. lemniscatus carvalhoi, M. lemniscatus lemniscatus, M. paraensis, M. spixii spixii, and M. surinamensis) were investigated, providing the most comprehensive, quantitative data on Micrurus venom composition to date, and more than tripling the number of Micrurus venom protein sequences previously available. The six venomes differ dramatically. All are dominated by 2–6 toxin classes that account for 91–99% of the toxin transcripts. The M. s. spixii venome is compositionally the simplest. In it, three-finger toxins (3FTxs) and phospholipases A2 (PLA2s) comprise >99% of the toxin transcripts, which include only four additional toxin families at levels ≥0.1%. Micrurus l. lemniscatus venom is the most complex, with at least 17 toxin families. However, in each venome, multiple structural subclasses of 3FTXs and PLA2s are present. These almost certainly differ in pharmacology as well. All venoms also contain phospholipase B and vascular endothelial growth factors. Minor components (0.1–2.0%) are found in all venoms except that of M. s. spixii. Other toxin families are present in all six venoms at trace levels (<0.005%). Minor and trace venom components differ in each venom. Numerous novel toxin chemistries include 3FTxs with previously unknown 8- and 10-cysteine arrangements, resulting in new 3D structures and target specificities. 9-cysteine toxins raise the possibility of covalent, homodimeric 3FTxs or heterodimeric toxins with unknown pharmacologies. Probable muscarinic sequences may be reptile-specific homologs that promote hypotension via vascular mAChRs. The first complete sequences are presented for 3FTxs putatively responsible for liberating glutamate from rat brain synaptosomes. Micrurus C-type lectin-like proteins may have 6–9 cysteine residues and may be monomers, or homo- or heterodimers of unknown pharmacology. Novel KSPIs, 3× longer than any seen previously, appear to have arisen in three species by gene duplication and fusion. Four species have transcripts homologous to the nociceptive toxin, (MitTx) α-subunit, but all six species had homologs to the β-subunit. The first non-neurotoxic, non-catalytic elapid phospholipase A2s are reported. All are probably myonecrotic. Phylogenetic analysis indicates that the six taxa diverged 15–35 million years ago and that they split from their last common ancestor with Old World elapines nearly 55 million years ago. Given their early diversification, many cryptic micrurine taxa are anticipated.

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“…A única exceção até o momento é a serpente Bothrops jararacussu, em que as PLA 2 s representaram a maior porção da peçonha (KASHIMA et al, 2004 CHANG 1996;WARD et al, 2001;YANG et al, 2003;ROBERTO et al, 2004;SETO et al, 2008). A proteína recombinante demonstrou a mesma sequência de aminoácidos, atividade fosfolipásica e efeito inibitório sobre plaquetas, observados para a proteína nativa BmooPLA 2 , sugerindo que a recPLA 2 foi eficientemente expressa, purificada e enovelada.…”

Section: Clonagem E Expressão Da Enzima Recombinanteunclassified

“…A expressão de proteínas recombinantes tornou-se uma importante ferramenta no auxílio do estudo da relação estrutura-função das PLA 2 s. Diversos experimentos desse tipo têm sido realizados na tentativa de melhor entender a relação estrutura-função das PLA 2 s de peçonhas de serpentes (CHANG;WU;CHANG, 1996;LIZANO et al, 2001;WARD et al, 2001;YANG et al, 2003 A seguir, foi avaliada a capacidade das toxinas em induzir a expressão de COX-2. A literatura mostra que a expressão de COX-2 é dependente da ativação transcricional, induzida por mediadores pró-inflamatórios (WU, 2005).…”

Section: Clonagem E Expressão Da Enzima Recombinanteunclassified

Caracterização funcional e estrutural de uma nova fosfolipase A2 ácida de Bothrops moojeni

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Functional expression and characterization of a recombinant phospholipase A2 from sea snake Lapemis hardwickii as a soluble protein in E. coli

Cited by 21 publications

References 30 publications

Two color morphs of the pelagic yellow-bellied sea snake, Pelamis platura, from different locations of Costa Rica: Snake venomics, toxicity, and neutralization by antivenom

Two color morphs of the pelagic yellow-bellied sea snake, Pelamis platura, from different locations of Costa Rica: Snake venomics, toxicity, and neutralization by antivenom

Coralsnake Venomics: Analyses of Venom Gland Transcriptomes and Proteomes of Six Brazilian Taxa

Caracterização funcional e estrutural de uma nova fosfolipase A2 ácida de Bothrops moojeni

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